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Variant Discovery in High-Throughput Sequencing Data

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Developed in the Data Sciences Platform at the Broad Institute, the toolkit offers a wide variety of tools with a primary focus on variant discovery and genotyping. Its powerful processing engine and high-performance computing features make it capable of taking on projects of any size. Learn more

Problem with DenoiseReadCounts

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3 comments

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    Gökalp Çelik

    Hi Sara Franzelli

    Can you clarify whether you trying to plot the results of a gCNV workflow or a Somatic CNV workflow?

     

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    Wenhong Jiang

    I have similar problem while plotting the results of a Somatic CNV workflow.

    HDF5-DIAG: Error detected in HDF5 (1.8.14) thread 0:
      #000: /mnt/scr1/abyrne/HDFJava-platypus-2.11/native/HDF5-prefix/src/HDF5/src/H5D.c line 358 in H5Dopen2(): not found
        major: Dataset
        minor: Object not found
      #001: /mnt/scr1/abyrne/HDFJava-platypus-2.11/native/HDF5-prefix/src/HDF5/src/H5Gloc.c line 430 in H5G_loc_find(): can't find object
        major: Symbol table
        minor: Object not found
      #002: /mnt/scr1/abyrne/HDFJava-platypus-2.11/native/HDF5-prefix/src/HDF5/src/H5Gtraverse.c line 861 in H5G_traverse(): internal path traversal failed
        major: Symbol table
        minor: Object not found
      #003: /mnt/scr1/abyrne/HDFJava-platypus-2.11/native/HDF5-prefix/src/HDF5/src/H5Gtraverse.c line 755 in H5G_traverse_real(): component not found
        major: Symbol table
        minor: Object not found
    HDF5-DIAG: Error detected in HDF5 (1.8.14) thread 0:
      #000: /mnt/scr1/abyrne/HDFJava-platypus-2.11/native/HDF5-prefix/src/HDF5/src/H5D.c line 358 in H5Dopen2(): not found
        major: Dataset
        minor: Object not found
      #001: /mnt/scr1/abyrne/HDFJava-platypus-2.11/native/HDF5-prefix/src/HDF5/src/H5Gloc.c line 430 in H5G_loc_find(): can't find object
        major: Symbol table
        minor: Object not found
      #002: /mnt/scr1/abyrne/HDFJava-platypus-2.11/native/HDF5-prefix/src/HDF5/src/H5Gtraverse.c line 861 in H5G_traverse(): internal path traversal failed
        major: Symbol table
        minor: Object not found
      #003: /mnt/scr1/abyrne/HDFJava-platypus-2.11/native/HDF5-prefix/src/HDF5/src/H5Gtraverse.c line 755 in H5G_traverse_real(): component not found
        major: Symbol table
        minor: Object not found

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    Gökalp Çelik

    Hi Wenhong Jiang

    You may be facing with a wrong file format or a corrupt file which results in this error. Can you check your hdf5 files with hdfview tool to see if the data is present. 

    https://www.hdfgroup.org/download-hdfview/

    Also your PoN and sample may not be compatible therefore you may be facing a similar issue.

    Regards. 

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