Keep only reads representing primary alignments (those that satisfy both the NotSecondaryAlignment and NotSupplementaryAlignment filters, or in terms of SAM flag values, must have neither of the 0x100 or 0x800 flags set).
Category Read Filters
Overview
Keep only reads representing primary alignments (those that satisfy both the NotSecondaryAlignment and NotSupplementaryAlignment filters, or in terms of SAM flag values, must have neither of the 0x100 or 0x800 flags set).Note that this filter represents a stronger criteria for "primary alignment" than the SAM flag 0x100 (representing ""not primary alignment" in some contexts).
For example, a read that has only the supplementary flag (0x800) set, but not the secondary (0x100) flag will be filtered out from processing by the PrimaryLineReadFilter, but would NOT be filtered out by other software that uses the looser notion of "not primary" that only depends on the "secondary" flag being set.
GATK version 4.0.1.0 built at 27-28-2019 10:28:59.
0 comments
Please sign in to leave a comment.