AMN
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`FilterMutectCalls` for normal only calls
AnsweredWhy do I see that the guide for `CreateSomaticPanelOfNormals` is `FilterMutectCalls` not used after calling germline `Mutect2` variants? Is this just a suggestion, and how strong is that suggestion...
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producing strandedness metrics from rnaseq data
AnsweredI have what i believe to be unstranded RNA data, and when I ran CollectRnaseqMetrics with STRAND=NONE I got absolutely no strandedness metrics, which I would need to confirm that my sample is indee...
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ApplyBQSRSpark ApplyBQSR has different results
I am seeing a different result when i use ApplyBQSR vs ApplyBQSRSpark, using the exact same inputs. I tried with both GATK 4.1.0.0 and 4.1.9.0 i am comparing the command: gatk ApplyBQSR --java...
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Comparison between ReadsPipelineSpark and MarkDuplicates
GATK version: 4.1.0.0 and 4.1.9.0Comparing two processes:``` gatk MarkDuplicates \ --TMP_DIR ./ \ --MAX_RECORDS_IN_RAM 50000 \ --INPUT ${inputBam} \ --METRICS_FILE ${idSampl...