1. Community
2. Webinar Discussion: Mutect2 [Nov 2020]
3. How do I prepare pon from my own data?

If I have paired tumor and PBMC samples, can I use the PBMC to create PON, and using the PON to analyze the paired tumor and PBMC samples?  

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• Ian Yi-Feng Chang
• November 17, 2020 15:57
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1. Community
2. Webinar Discussion: Mutect2 [Nov 2020]
3. Is it correct to use Mutect2 to identify somatic variants from cell free DNA or from circulating tumour cells?

Hi, fleharty, Thank you for your reply. The link to "Mutect2 Read Orientation Artifacts Workflow" is https://gatk.broadinstitute.org/hc/en-us/articles/360035531132 Please search "A step-by-step gui...

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• Ian Yi-Feng Chang
• November 17, 2020 03:39
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1. Community
2. Webinar Discussion: Mutect2 [Nov 2020]
3. Is it correct to use Mutect2 to identify somatic variants from cell free DNA or from circulating tumour cells?

In the Mutect2 Read Orientation Artifacts Workflow, can I use normal FFPE and tumor FFPE samples from the same patients to call somatic variants? 

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• Ian Yi-Feng Chang
• November 17, 2020 01:51
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