rohit satyam
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Activity overview
Latest activity by rohit satyam-
rohit satyam commented,
Genevieve Brandt (she/her) The Dragmap aligner isn't working properly anymore and when issues are posted on GitHub they aren't being attended to lately. It's been 2 months now. Since DragMap is DRA...
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rohit satyam commented,
Hi Anthony Dias-Ciarla. It's been a month now. Is there any update from your team? Genevieve Brandt (she/her) Can you help me, please? I have long waited to get a "yes" or "np" reply to my query ab...
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rohit satyam commented,
Hi. I was wondering if the GATK team was able to find time to review this query. I read your support policy and my question seems to be in the lowest priority category. But we are planning to submi...
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rohit satyam created a post,
Using gatk VariantFiltration to filter ONT wf-artic generated VCF files
Will it be valid to use gatk VariantFiltration on the variant calls generated using wf-artic pipeline? I am asking this because we have both Illumina and ONT runs for few samples and we wish to che...
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rohit satyam created a post,
Should we run MarkDuplicates on PCR-Free Libraries made using PCR-Free Kits to take care of Optical Duplicates ?
I found a post here that states we shouldn't run MarkDuplicates on the data produced using PCR-Free Kits. So should we use it to mark optical duplicates because Illumina platform generates a lot of...
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rohit satyam commented,
Hi Genevieve Brandt (she/her). Thanks for your input. We suspected something to be fishy with the demultiplexing step and it turns out to be true. We got it demultiplexed it again and now it works ...
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rohit satyam commented,
Hi Genevieve Brandt (she/her) My BWA command ran successfully on 9 pairs of the data without any error. The new preprocessing pipeline of GATK as mentioned here and the one you mentioned should pr...
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rohit satyam created a post,
SAMException: Value was put into PairInfoMap error even after using -M flag
Hi Everyone. I am facing a nontrivial issue at the markduplicates step in all the 14 pairs (samples of cancer). I keep on getting PairInfoMap error in all of my samples because of some unknown reas...
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rohit satyam commented,
Hi, is there any update on the proper documentation of the Mitochondrial Variant Analysis. I am really finding it difficult to understand really what sequence of steps must be followed for finding ...
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rohit satyam commented,
Hi Sir I have currently 23 Tumor vs Normal samples. According to GATK Best Practices, it is desirable if not necessary to include PONS as described here and here. My question is would PONS using 23...