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Shivangi Agarwal

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Latest activity by Shivangi Agarwal
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    Shivangi Agarwal commented,

    Okay, thanks. I am looking into it and will get back to you soon.       Thanks, Shivangi

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    Shivangi Agarwal created a post,

    Mutect2 -could not detect expected Indels

    REQUIRED for all errors and issues: a) GATK version used: b) Exact command used: c) Entire program log: Hi GATK team, I am calling somatic variants using Mutect2 for 10 samples. I could see lots o...

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    Shivangi Agarwal commented,

    Hi David,  Thanks so much for your response. I did call variants both ways (1st: no germline and no public PON and IInd: Germline: somatic-b37_af-only-gnomad.raw.sites.vcf & PON somatic-b37_Mutect2...

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    Shivangi Agarwal created a post,

    Huge number of variants in tumor only mode as compared to T/N paired mode using Mutect2

    REQUIRED for all errors and issues: a) GATK version used:4.2.2.0b) Exact command used: c) Entire program log: Hi GATK team, I want to call somatic variants for 60 samples in tumor-normal paired mo...

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    Shivangi Agarwal created a post,

    af-only-gnomad.vcf.gz

    REQUIRED for all errors and issues: a) GATK version used: b) Exact command used: c) Entire program log: Hi GATK experts, I want to download af-only-gnomad.vcf.gz to CreateSomaticPanelsofNormals. I...

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    Shivangi Agarwal commented,

    Hi Genevieve, I have a few questions regarding my GATK run set up. These are as: 1. I have 12 normal samples. Can I make my own PON taking those twelve ? I mean is it allowed or recommended ? Does ...

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    Shivangi Agarwal commented,

    Hey Genevieve Brandt, Can you please spare some time to answer my query as mentioned above ? I need to run this analysis at the earliest.     Thanks and regards Shivangi Agarwal

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    Shivangi Agarwal created a post,

    Variant calling for matched samples

    Answered

    Hi, I want to call variants for my 36 samples each of which is having normal data. The samples are matched normal. Please let me know how to correctly run GATK and what are the steps for this ? My ...

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    Shivangi Agarwal commented,

    Hi Genevieve,   Can you please respond to the above query.   Thanks

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    Shivangi Agarwal commented,

    Hi Genevieve, I want to call variants for my samples which are divided into four categories as normal, stage I, stage II and stage III. My understanding till now after going through multiple docume...