cjz
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Latest activity by cjz-
cjz created a post,
mutect2.bam could not find the mutation, but bqsr found the mutation
AnsweredI use a bed to find mutations and use the mutect2 process. Normal samples and tumor samples use mutect2 for mutation detection. After importing the bam file of bqsr into IGV, I found that this site...
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cjz commented,
I use the same pon, but the bed file for mutect2 to detect mutations is different. Does this have an impact?
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cjz created a post,
Different panels detect the same site, there are two different annotations, somatic and germline
I used mutect2 to detect mutations, and used FilterMutectCalls to detect mutations. The first time I used a panel to detect mutations, the site was somatic mutation (PASS), but the second time I us...