Nagarajan Raju
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Activity overview
Latest activity by Nagarajan Raju-
Nagarajan Raju created a post,
Extracting gene segments that is in anti-sense/negative strand
Hi, I am trying to extract gene sequences using the bcftools consensus command. How to exactly extract the gene sequences located in the negative strand? For example, genex is located in the negati...
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Nagarajan Raju created a post,
variant calling: dict and indexing
Hi, For variant calling suing Haplotypecaller, I created dict and indexing for the reference genome. Should I create index files for the input genome (bam) file also? Also, should I sort the bam fi...
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Nagarajan Raju created a post,
Gene annonation from aligned genome with reference
Hi, I have bam file of an genome sequence (query) aligned with reference genome. I would like to extract few gene sequences. It will be helpful if someone point out how to proceed this and what...
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Nagarajan Raju created a post,
Genome sequence analysis: preprocessing to variant analysis
Hi, I am new to genome analysis and would like to learn from preprocessing to variant analysis. It will be helpful if someone point out how to start and what are basics protocol/documents to rea...