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Genome Analysis Toolkit

Variant Discovery in High-Throughput Sequencing Data

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Developed in the Data Sciences Platform at the Broad Institute, the toolkit offers a wide variety of tools with a primary focus on variant discovery and genotyping. Its powerful processing engine and high-performance computing features make it capable of taking on projects of any size. Learn more

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Non-Human

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Show all All Planned Not planned Completed Answered No status Sort by newest post Newest post Recent activity Votes Comments
  • AnalyzeCovaiates BQSR plots on canine data
    • Kate Megquier
    • November 13, 2020 22:14
    0 votes 3 comments
  • Disable strandbias
    • Wen Huang
    • November 13, 2020 20:34
    0 votes 1 comment
  • False positive Indel calling with GATK v4.1.4.0
    • ali pirani
    • November 12, 2020 15:14
    0 votes 1 comment
  • ApplyBQSR reduces reads quality
    • Khalil Jahed
    • November 11, 2020 21:46
    0 votes 3 comments
  • Getting an error with genomicsdbimport
    • Vinod Kumar
    • November 09, 2020 16:35
    • Edited
    0 votes 9 comments
  • Missing MQ scores in gvcf
    • Heather Tuttle
    • October 26, 2020 22:22
    0 votes 7 comments
  • Why does GATK haplotype caller calls SNPs from soft-clipped bases?
    • AlibiKr
    • October 13, 2020 14:46
    0 votes 1 comment
  • Access to outdated forum discussion
    • Begonia_pavonina
    • September 25, 2020 11:41
    0 votes 2 comments
  • Active Region Re-assembly Answered
    • Ellen Weinheimer
    • September 21, 2020 14:54
    0 votes 2 comments
  • GenotypeGVCFs stalls while using --all-sites Answered
    • Rachael Bay
    • September 15, 2020 22:13
    0 votes 37 comments
  • HaplotypeCaller for haploid genome
    • Pummi Singh
    • September 06, 2020 23:30
    0 votes 7 comments
  • ApplyBQSR to unmapped reads for non-model organism does not work with '-L unmapped' flag
    • cali
    • September 02, 2020 23:17
    0 votes 8 comments
  • No individual sample info in combineGVCF
    • Niyomi House
    • September 01, 2020 19:49
    0 votes 4 comments
  • USER ERROR has occurred: Sorry, we only support a single reads input for for this spark tool.
    • madzayasodara
    • August 27, 2020 22:21
    0 votes 1 comment
  • Missing value evaluate as fail
    • Laura
    • August 14, 2020 00:16
    0 votes 5 comments
  • Which exact info does BaseRecalibrator uses from a vcf?
    • madzayasodara
    • August 12, 2020 00:28
    0 votes 7 comments
  • Haplotype caller error; java.lang.IllegalStateException: Graph must have ref source and sink vertices Answered
    • New_gatk_user
    • August 11, 2020 15:12
    0 votes 6 comments
  • Cannot run through CNVDiscoverypipeline
    • Jinpeng Wang
    • August 08, 2020 14:55
    0 votes 5 comments
  • NumberFormatException Error in VariantFiltration
    • Erin C
    • August 08, 2020 00:00
    0 votes 3 comments
  • Excessive heterozygosity in haplotypecaller
    • Mazd_Faa
    • August 06, 2020 18:29
    0 votes 3 comments
  • A USER ERROR has occurred: Badly formed genome unclippedLoc: Parameters to GenomeLocParser are incorrect:The stop position 0 is less than start 1 in contig contig004333 Answered
    • New_gatk_user
    • August 04, 2020 11:43
    • Edited
    0 votes 8 comments
  • There is insufficient memory for the Java Runtime Enviroment to continue when running CNVDiscoveryPipeline
    • Jinpeng Wang
    • August 03, 2020 22:48
    0 votes 3 comments
  • GenomeStrip CNVDiscoveryPipeline: merged.brig.vcf: Too many open files, for input source: 08.strip/CNV/cnv_stage7/seq_chr28/P4019/seq_chr28.merged.brig.vcf
    • Jinpeng Wang
    • August 03, 2020 21:52
    0 votes 3 comments
  • Failed in an LSF library call when running CNVDiscoveryPipeline of GenomeStrip. and failure when merging brig.vcfs
    • Jinpeng Wang
    • August 02, 2020 15:05
    0 votes 2 comments
  • java.lang.IllegalArgumentException: MALFORMED
    • Suma Bala Dabi
    • July 31, 2020 22:04
    0 votes 3 comments
  • Alignment file does not exist: 08.strip/metadata/headers.bam when running GenomeStrip SVPreprocess
    • Jinpeng Wang
    • July 31, 2020 10:37
    • Edited
    0 votes 7 comments
  • Calculating the empirical allele frequency
    • New_gatk_user
    • July 30, 2020 08:41
    0 votes 2 comments
  • Selectvariants - specific region
    • wbsimey
    • July 18, 2020 17:37
    0 votes 2 comments
  • Likely true variants failing MQ40 filter in joint genotyping
    • Aditi Batra
    • July 15, 2020 11:44
    0 votes 2 comments
  • Variant calling in Fish RNA-seq
    • Elizabeth Schabot
    • July 14, 2020 16:55
    0 votes 3 comments
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