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Variant Discovery in High-Throughput Sequencing Data

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Developed in the Data Sciences Platform at the Broad Institute, the toolkit offers a wide variety of tools with a primary focus on variant discovery and genotyping. Its powerful processing engine and high-performance computing features make it capable of taking on projects of any size. Learn more

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  • RemoveNearbyIndels: CRSP
    • Andrew Zhang
    • May 13, 2020 06:49
    0 votes 6 comments
  • HaplotypeCaller on multiple BAMs vs Joint-Call Cohort
    • lahl
    • May 12, 2020 20:21
    0 votes 3 comments
  • Output vcf file from HaplotypeCaller mostlly contain <NON_REF> alteration
    • Jay Singh
    • May 12, 2020 14:58
    0 votes 3 comments
  • Can't add to PATH
    • Linda Do
    • May 12, 2020 04:46
    0 votes 2 comments
  • Equations in AS_StrandOddsRatio aren't shown correctly
    • Leo Hsiao
    • May 12, 2020 01:19
    0 votes 1 comment
  • Error with GATK 4 and R gsalib
    • Oscar Sotolongo-Grau
    • May 11, 2020 09:45
    0 votes 2 comments
  • ERROR while calling variants...
    • Manoj Kumar
    • May 10, 2020 03:23
    • Edited
    0 votes 2 comments
  • Character cannot be cast to java.lang.Integer Exception
    • Daniel Boiarsky
    • May 07, 2020 14:44
    0 votes 1 comment
  • Picard CollectHsMetrics PCT_EXC_OFF_TARGET
    • Holly Corbitt
    • May 07, 2020 04:09
    1 vote 4 comments
  • LiftoverVcf fails to lift over a GVCF file. Error java.lang.ArrayIndexOutOfBoundsException: -1
    • jorgez
    • May 06, 2020 12:54
    0 votes 10 comments
  • Paired-End Reads and Variant Calling
    • Lauren Fedenia
    • May 05, 2020 13:52
    0 votes 1 comment
  • ReciprocalOverlapAnnotator error for Sniffles data
    • Ghausia Begum
    • May 04, 2020 20:02
    0 votes 2 comments
  • Problem Installing and testing GATK-4.1.7.0
    • Arjun Upadhaya
    • May 04, 2020 06:34
    1 vote 5 comments
  • A USER ERROR has occurred: Number of read groups must be >= 1, but is 0
    • Jay Singh
    • May 01, 2020 17:24
    0 votes 4 comments
  • Do I need to perform fastqc and adapters trimming before gatk pipeline?
    • LG
    • May 01, 2020 02:49
    1 vote 4 comments
  • CombineGVCFs Fails for a batch of 2 samples where samples shows high number of alternate INDEL Alleles
    • info 2020
    • April 29, 2020 20:24
    0 votes 3 comments
  • Read Group: ID for split files of multiple samples on multiple lanes
    • Himawari
    • April 28, 2020 07:34
    • Edited
    0 votes 7 comments
  • Workaround for Missing Platform Identifier in BQSRPipelineSpark?
    • Robert Bremel
    • April 27, 2020 21:17
    0 votes 1 comment
  • Posts pending approval now for 1 month
    • mack812
    • April 27, 2020 04:05
    • Edited
    0 votes 1 comment
  • Unable to replicate the spanning deletions documentation
    • Saurabh Parikh
    • April 27, 2020 01:55
    • Edited
    0 votes 8 comments
  • How to set a COMPRESSION_LEVEL of ApplyBQSR
    • Nickier
    • April 26, 2020 10:34
    0 votes 6 comments
  • Error while running HaplotypeCaller with gatk 4
    • Venkateswara Swamy
    • April 24, 2020 17:27
    0 votes 1 comment
  • Broken: conda env create -n gatk -f gatkcondaenv.yml
    • James White
    • April 24, 2020 17:01
    0 votes 15 comments
  • Trouble Installing GTAK
    • Taylor Burke
    • April 24, 2020 12:24
    0 votes 2 comments
  • I have no idea why error occurs...(also used java8)
    • 신정원
    • April 24, 2020 06:26
    0 votes 1 comment
  • error outpout when i Evaluated the depth of coverage of the aligned region
    • Sanae Borrohou
    • April 23, 2020 14:37
    0 votes 1 comment
  • GATK4 HaplotypeCaller ERROR : expected haplotypes.size() >= eventsAtThisLoc.size() + 1
    • Şevval Aktürk
    • April 23, 2020 12:26
    0 votes 12 comments
  • gatk4-output from baserecalibrator and apply BQSR
    • shuMSR
    • April 22, 2020 10:42
    0 votes 1 comment
  • ValidateSamFile tmp not found ERROR with not output or Exception
    • James
    • April 20, 2020 23:34
    0 votes 4 comments
  • Typo in VQSR tutorial
    • warren kretzschmar
    • April 20, 2020 12:43
    0 votes 1 comment
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