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Variant Discovery in High-Throughput Sequencing Data

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Developed in the Data Sciences Platform at the Broad Institute, the toolkit offers a wide variety of tools with a primary focus on variant discovery and genotyping. Its powerful processing engine and high-performance computing features make it capable of taking on projects of any size. Learn more

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Show all All Planned Not planned Completed Answered No status Sort by newest post Newest post Recent activity Votes Comments
  • The mutect ERROR:Please add an explicit type tag :NAME listing the correct type from among the supported types Answered
    • 灵己 孟
    • June 28, 2021 03:15
    0 votes 3 comments
  • Error while running CreateReadCountPanelOfNormals with gatk-4.1.4.1 Answered
    • Alice Shen
    • June 10, 2021 07:36
    0 votes 3 comments
  • MuTect2 Mismatch between the reference haplotype and the reference assembly graph path. Answered
    • Heejun Jang
    • June 07, 2021 02:53
    0 votes 1 comment
  • The output of Mutect2 cannot be accepted by FilterMutectCalls in GATK-4.2.0.0 Answered
    • Qihan Long
    • June 04, 2021 03:25
    • Edited
    0 votes 10 comments
  • The depth of SNV/InDels calculated by Mutect2 in GATK4.2.0.0 is much lower than real sequencing depth, why? Answered
    • Ce Wang
    • May 25, 2021 06:08
    • Edited
    0 votes 19 comments
  • How do I set less stringent germline filtering in Mutect2 / filtermutectcalls, many false negatives Answered
    • Tjitske de Vries
    • May 21, 2021 09:47
    0 votes 1 comment
  • Mysterious Funcotator issue Answered
    • Robert Bremel
    • May 21, 2021 01:22
    0 votes 8 comments
  • MergeBamAlignment produces unknown error Answered
    • vctrymao
    • May 18, 2021 16:19
    • Edited
    0 votes 4 comments
  • CollectAllelicCounts- Exception in thread "main" java.lang.OutOfMemoryError: GC overhead limit exceeded Answered
    • rahelp
    • May 16, 2021 13:17
    0 votes 12 comments
  • IlluminaBasecallsToSam does not demultiplex NovaSeq barcoded reads Answered
    • myourshaw
    • May 10, 2021 21:46
    • Edited
    0 votes 5 comments
  • java.lang.OutofMemoryError: GC overhead limit exceeded Answered
    • Jack Prazich
    • May 03, 2021 16:54
    0 votes 28 comments
  • GenomicsDBImport does not support GVCFs with MNPs Answered
    • Rodolfo Adrián Arriaga Rivera
    • April 23, 2021 16:52
    • Edited
    0 votes 1 comment
  • Mutect2
    • Sotaro Kanematsu
    • April 14, 2021 07:24
    0 votes 1 comment
  • Badly formed genome unclippedLoc: Contig NC_007605 given as location, but this contig isn't present in the Fasta sequence dictionary Answered
    • rahelp
    • April 12, 2021 13:36
    0 votes 7 comments
  • Why do a clear expected variant not show up in the Mutect2 vcf file Answered
    • Nabeel Ahmed
    • April 07, 2021 09:13
    0 votes 14 comments
  • CreateSomaticPanelOfNormals ALT allele bug Answered
    • G E
    • April 02, 2021 15:19
    • Edited
    0 votes 10 comments
  • Funcotator data source not correct Answered
    • Jamie Xie
    • March 29, 2021 20:42
    • Edited
    0 votes 13 comments
  • Issue Regarding CNV_Somatic_Pair Workflow
    • Hanbing Song
    • March 23, 2021 00:02
    0 votes 0 comments
  • Mutect2 failing to produce F1R2.tar.gz files on TCGA bam (tumor-normal pairs) Answered
    • TMB
    • March 10, 2021 17:29
    0 votes 5 comments
  • Variant called in reads from ArtificialHaplotypeRG Answered
    • yingchen69
    • March 09, 2021 05:21
    0 votes 4 comments
  • Picard CrosscheckFingerprint: Sequence dictionaries are not the same size Answered
    • Doris Fu
    • March 08, 2021 20:17
    • Edited
    0 votes 3 comments
  • FilterAlignmentArtifacts - only emitting PASS variants Answered
    • GE
    • March 01, 2021 15:38
    0 votes 3 comments
  • Missing documentation on strict_strand Answered
    • GE
    • February 28, 2021 19:15
    • Edited
    0 votes 4 comments
  • strand_bias filtering is never emitted Answered
    • GE
    • February 28, 2021 18:11
    • Edited
    0 votes 7 comments
  • FilterMutectCalls didn't filtered any variants for my vcf file Answered
    • Xiya Song
    • February 28, 2021 11:02
    0 votes 3 comments
  • Mutect2 - Joint calling Answered
    • Yen-Ping Yeh
    • February 23, 2021 08:44
    0 votes 8 comments
  • HaplotypeCaller VCF for PON
    • Steven Bentley
    • February 22, 2021 23:05
    0 votes 3 comments
  • 1 year-old bug in libgkl that has not been fixed affecting FilterAlignmentArtifacts and HaplotypeCaller
    • GE
    • February 18, 2021 23:16
    0 votes 3 comments
  • Bug: Badly formed genome unclippedLoc
    • GE
    • February 16, 2021 22:43
    0 votes 12 comments
  • FilterMutectCalls --unique-alt-read-count argument
    • Greg Goralogia
    • February 16, 2021 20:48
    0 votes 10 comments
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