ASEReadCount Only Outputting the First Chromosome
AnsweredREQUIRED for all errors and issues:
a) GATK version used:
b) Exact command used:
c) Entire program log:
REQUIRED for all errors and issues:
a) GATK version used: GATK version used: gatk-4.2.6.1
b) Exact command used:
#!/bin/bash
#PBS -l nodes=1:pamunkey:ppn=24
#PBS -l walltime=30:00:00
#PBS -N ASE
#PBS -o /ches/data10/lgspeight/results/ASE/ASE_output
#PBS -e /ches/data10/lgspeight/results/ASE/ASE_error
#PBS -t 1-48%3
REF=/ches/data10/lgspeight/RNA-Seq/01_rawdata/genome/Clam_genome.fna
/ches/home00/lgspeight/gatk-4.2.6.1/gatk CreateSequenceDictionary -R $REF
WORKDIR=/ches/data10/lgspeight/results/ASE
cd $WORKDIR
BAMDIR=/ches/data10/lgspeight/results/Align
VCFDIR=/ches/data10/lgspeight/results/SNP_Call/SNPS
ACCLIST=/ches/data10/lgspeight/RNA-Seq/01_rawdata/accessionlist.txt
NUM=$(expr ${PBS_ARRAYID})
SAMPLE=$(sed -n ${NUM}p $ACCLIST)
/ches/home00/lgspeight/gatk-4.2.6.1/gatk IndexFeatureFile -I $VCFDIR/$SAMPLE-var.final.vcf.gz
PICARD=/ches/home00/lgspeight/picard-2.27.4/picard/build/libs/picard.jar
java -jar $PICARD AddOrReplaceReadGroups \
I=$BAMDIR/${SAMPLE}.bam \
O=$BAMDIR/${SAMPLE}.2.bam \
RGID=4 \
RGLB=lib1 \
RGPL=ILLUMINA \
RGPU=unit1 \
RGSM=20
/ches/home00/lgspeight/gatk-4.2.6.1/gatk --java-options '-DGATK_STACKTRACE_ON_USER_EXCEPTION=true' ASEReadCounter \
-R $REF \
-I $BAMDIR/${SAMPLE}.2.bam \
-V $VCFDIR/$SAMPLE-var.final.vcf.gz \
-O $WORKDIR/${SAMPLE}.table
c) Entire program log:
Using GATK jar /ches/home00/lgspeight/gatk-4.2.6.1/gatk-package-4.2.6.1-local.jar
Running:
java -Dsamjdk.use_async_io_read_samtools=false -Dsamjdk.use_async_io_write_samtools=true -Dsamjdk.use_async_io_write_tribble=false -Dsamjdk.compression_level=2 -jar /ches/home00/lgspeight/gatk-4.2.6.1/gatk-package-4.2.6.1-local.jar CreateSequenceDictionary -R /ches/data10/lgspeight/RNA-Seq/01_rawdata/genome/Clam_genome.fna
INFO 2022-08-25 10:10:29 CreateSequenceDictionary Output dictionary will be written in /ches/data10/lgspeight/RNA-Seq/01_rawdata/genome/Clam_genome.dict
10:10:29.453 INFO NativeLibraryLoader - Loading libgkl_compression.so from jar:file:/ches/home00/lgspeight/gatk-4.2.6.1/gatk-package-4.2.6.1-local.jar!/com/intel/gkl/native/libgkl_compression.so
[Thu Aug 25 10:10:29 EDT 2022] CreateSequenceDictionary --REFERENCE /ches/data10/lgspeight/RNA-Seq/01_rawdata/genome/Clam_genome.fna --TRUNCATE_NAMES_AT_WHITESPACE true --NUM_SEQUENCES 2147483647 --VERBOSITY INFO --QUIET false --VALIDATION_STRINGENCY STRICT --COMPRESSION_LEVEL 2 --MAX_RECORDS_IN_RAM 500000 --CREATE_INDEX false --CREATE_MD5_FILE false --GA4GH_CLIENT_SECRETS client_secrets.json --help false --version false --showHidden false --USE_JDK_DEFLATER false --USE_JDK_INFLATER false
[Thu Aug 25 10:10:29 EDT 2022] Executing as lgspeight@pm01.hpc.vims.edu on Linux 3.10.0-514.el7.x86_64 amd64; OpenJDK 64-Bit Server VM 1.8.0_292-b10; Deflater: Intel; Inflater: Intel; Provider GCS is available; Picard version: Version:4.2.6.1
[Thu Aug 25 10:10:29 EDT 2022] picard.sam.CreateSequenceDictionary done. Elapsed time: 0.00 minutes.
Runtime.totalMemory()=2151153664
To get help, see http://broadinstitute.github.io/picard/index.html#GettingHelp
picard.PicardException: /ches/data10/lgspeight/RNA-Seq/01_rawdata/genome/Clam_genome.dict already exists. Delete this file and try again, or specify a different output file.
at picard.sam.CreateSequenceDictionary.doWork(CreateSequenceDictionary.java:220)
at picard.cmdline.CommandLineProgram.instanceMain(CommandLineProgram.java:308)
at org.broadinstitute.hellbender.cmdline.PicardCommandLineProgramExecutor.instanceMain(PicardCommandLineProgramExecutor.java:37)
at org.broadinstitute.hellbender.Main.runCommandLineProgram(Main.java:160)
at org.broadinstitute.hellbender.Main.mainEntry(Main.java:203)
at org.broadinstitute.hellbender.Main.main(Main.java:289)
Using GATK jar /ches/home00/lgspeight/gatk-4.2.6.1/gatk-package-4.2.6.1-local.jar
Running:
java -Dsamjdk.use_async_io_read_samtools=false -Dsamjdk.use_async_io_write_samtools=true -Dsamjdk.use_async_io_write_tribble=false -Dsamjdk.compression_level=2 -jar /ches/home00/lgspeight/gatk-4.2.6.1/gatk-package-4.2.6.1-local.jar IndexFeatureFile -I /ches/data10/lgspeight/results/SNP_Call/SNPS/A_1_32-var.final.vcf.gz
10:10:32.889 INFO NativeLibraryLoader - Loading libgkl_compression.so from jar:file:/ches/home00/lgspeight/gatk-4.2.6.1/gatk-package-4.2.6.1-local.jar!/com/intel/gkl/native/libgkl_compression.so
10:10:33.058 INFO IndexFeatureFile - ------------------------------------------------------------
10:10:33.058 INFO IndexFeatureFile - The Genome Analysis Toolkit (GATK) v4.2.6.1
10:10:33.059 INFO IndexFeatureFile - For support and documentation go to https://software.broadinstitute.org/gatk/
10:10:33.059 INFO IndexFeatureFile - Executing as lgspeight@pm01.hpc.vims.edu on Linux v3.10.0-514.el7.x86_64 amd64
10:10:33.059 INFO IndexFeatureFile - Java runtime: OpenJDK 64-Bit Server VM v1.8.0_292-b10
10:10:33.059 INFO IndexFeatureFile - Start Date/Time: August 25, 2022 10:10:32 AM EDT
10:10:33.059 INFO IndexFeatureFile - ------------------------------------------------------------
10:10:33.060 INFO IndexFeatureFile - ------------------------------------------------------------
10:10:33.060 INFO IndexFeatureFile - HTSJDK Version: 2.24.1
10:10:33.060 INFO IndexFeatureFile - Picard Version: 2.27.1
10:10:33.061 INFO IndexFeatureFile - Built for Spark Version: 2.4.5
10:10:33.061 INFO IndexFeatureFile - HTSJDK Defaults.COMPRESSION_LEVEL : 2
10:10:33.061 INFO IndexFeatureFile - HTSJDK Defaults.USE_ASYNC_IO_READ_FOR_SAMTOOLS : false
10:10:33.061 INFO IndexFeatureFile - HTSJDK Defaults.USE_ASYNC_IO_WRITE_FOR_SAMTOOLS : true
10:10:33.061 INFO IndexFeatureFile - HTSJDK Defaults.USE_ASYNC_IO_WRITE_FOR_TRIBBLE : false
10:10:33.061 INFO IndexFeatureFile - Deflater: IntelDeflater
10:10:33.061 INFO IndexFeatureFile - Inflater: IntelInflater
10:10:33.062 INFO IndexFeatureFile - GCS max retries/reopens: 20
10:10:33.062 INFO IndexFeatureFile - Requester pays: disabled
10:10:33.062 INFO IndexFeatureFile - Initializing engine
10:10:33.062 INFO IndexFeatureFile - Done initializing engine
10:10:33.581 INFO FeatureManager - Using codec VCFCodec to read file file:///ches/data10/lgspeight/results/SNP_Call/SNPS/A_1_32-var.final.vcf.gz
10:10:33.682 INFO ProgressMeter - Starting traversal
10:10:33.683 INFO ProgressMeter - Current Locus Elapsed Minutes Records Processed Records/Minute
10:10:39.116 WARN IntelInflater - Zero Bytes Written : 0
10:10:39.119 INFO ProgressMeter - NW_025543028.1:13539 0.1 755807 8346847.0
10:10:39.119 INFO ProgressMeter - Traversal complete. Processed 755807 total records in 0.1 minutes.
10:10:39.201 INFO IndexFeatureFile - Successfully wrote index to /ches/data10/lgspeight/results/SNP_Call/SNPS/A_1_32-var.final.vcf.gz.tbi
10:10:39.202 INFO IndexFeatureFile - Shutting down engine
[August 25, 2022 10:10:39 AM EDT] org.broadinstitute.hellbender.tools.IndexFeatureFile done. Elapsed time: 0.11 minutes.
Runtime.totalMemory()=2871001088
INFO 2022-08-25 10:10:39 AddOrReplaceReadGroups
********** NOTE: Picard's command line syntax is changing.
**********
********** For more information, please see:
**********
**********
********** The command line looks like this in the new syntax:
**********
********** AddOrReplaceReadGroups -I /ches/data10/lgspeight/results/Align/A_1_32.bam -O /ches/data10/lgspeight/results/Align/A_1_32.2.bam -RGID 4 -RGLB lib1 -RGPL ILLUMINA -RGPU unit1 -RGSM 20
**********
10:10:40.436 INFO NativeLibraryLoader - Loading libgkl_compression.so from jar:file:/ches/home00/lgspeight/picard-2.27.4/picard/build/libs/picard.jar!/com/intel/gkl/native/libgkl_compression.so
[Thu Aug 25 10:10:40 EDT 2022] AddOrReplaceReadGroups INPUT=/ches/data10/lgspeight/results/Align/A_1_32.bam OUTPUT=/ches/data10/lgspeight/results/Align/A_1_32.2.bam RGID=4 RGLB=lib1 RGPL=ILLUMINA RGPU=unit1 RGSM=20 VERBOSITY=INFO QUIET=false VALIDATION_STRINGENCY=STRICT COMPRESSION_LEVEL=5 MAX_RECORDS_IN_RAM=500000 CREATE_INDEX=false CREATE_MD5_FILE=false GA4GH_CLIENT_SECRETS=client_secrets.json USE_JDK_DEFLATER=false USE_JDK_INFLATER=false
[Thu Aug 25 10:10:40 EDT 2022] Executing as lgspeight@pm01.hpc.vims.edu on Linux 3.10.0-514.el7.x86_64 amd64; OpenJDK 64-Bit Server VM 1.8.0_292-b10; Deflater: Intel; Inflater: Intel; Provider GCS is not available; Picard version: 2.27.4-SNAPSHOT
INFO 2022-08-25 10:10:40 AddOrReplaceReadGroups Created read-group ID=4 PL=ILLUMINA LB=lib1 SM=20
INFO 2022-08-25 10:10:54 AddOrReplaceReadGroups Processed 1,000,000 records. Elapsed time: 00:00:13s. Time for last 1,000,000: 13s. Last read position: NC_059305.1:61,973,973
INFO 2022-08-25 10:11:07 AddOrReplaceReadGroups Processed 2,000,000 records. Elapsed time: 00:00:25s. Time for last 1,000,000: 12s. Last read position: NC_059306.1:34,228,113
INFO 2022-08-25 10:11:19 AddOrReplaceReadGroups Processed 3,000,000 records. Elapsed time: 00:00:38s. Time for last 1,000,000: 12s. Last read position: NC_059306.1:89,574,840
INFO 2022-08-25 10:11:32 AddOrReplaceReadGroups Processed 4,000,000 records. Elapsed time: 00:00:51s. Time for last 1,000,000: 12s. Last read position: NC_059307.1:17,365,946
INFO 2022-08-25 10:11:45 AddOrReplaceReadGroups Processed 5,000,000 records. Elapsed time: 00:01:04s. Time for last 1,000,000: 12s. Last read position: NC_059307.1:82,331,975
INFO 2022-08-25 10:11:58 AddOrReplaceReadGroups Processed 6,000,000 records. Elapsed time: 00:01:17s. Time for last 1,000,000: 12s. Last read position: NC_059308.1:41,536,741
INFO 2022-08-25 10:12:11 AddOrReplaceReadGroups Processed 7,000,000 records. Elapsed time: 00:01:30s. Time for last 1,000,000: 12s. Last read position: NC_059309.1:5,773,276
INFO 2022-08-25 10:12:24 AddOrReplaceReadGroups Processed 8,000,000 records. Elapsed time: 00:01:43s. Time for last 1,000,000: 12s. Last read position: NC_059309.1:60,862,275
INFO 2022-08-25 10:12:37 AddOrReplaceReadGroups Processed 9,000,000 records. Elapsed time: 00:01:55s. Time for last 1,000,000: 12s. Last read position: NC_059310.1:7,372,107
INFO 2022-08-25 10:12:49 AddOrReplaceReadGroups Processed 10,000,000 records. Elapsed time: 00:02:08s. Time for last 1,000,000: 12s. Last read position: NC_059310.1:59,160,424
INFO 2022-08-25 10:13:02 AddOrReplaceReadGroups Processed 11,000,000 records. Elapsed time: 00:02:21s. Time for last 1,000,000: 12s. Last read position: NC_059311.1:9,873,652
INFO 2022-08-25 10:13:14 AddOrReplaceReadGroups Processed 12,000,000 records. Elapsed time: 00:02:33s. Time for last 1,000,000: 12s. Last read position: NC_059311.1:51,585,895
INFO 2022-08-25 10:13:27 AddOrReplaceReadGroups Processed 13,000,000 records. Elapsed time: 00:02:46s. Time for last 1,000,000: 12s. Last read position: NC_059311.1:89,128,595
INFO 2022-08-25 10:13:40 AddOrReplaceReadGroups Processed 14,000,000 records. Elapsed time: 00:02:59s. Time for last 1,000,000: 12s. Last read position: NC_059312.1:35,981,845
INFO 2022-08-25 10:13:52 AddOrReplaceReadGroups Processed 15,000,000 records. Elapsed time: 00:03:11s. Time for last 1,000,000: 12s. Last read position: NC_059312.1:75,200,341
INFO 2022-08-25 10:14:05 AddOrReplaceReadGroups Processed 16,000,000 records. Elapsed time: 00:03:24s. Time for last 1,000,000: 12s. Last read position: NC_059313.1:27,201,631
INFO 2022-08-25 10:14:18 AddOrReplaceReadGroups Processed 17,000,000 records. Elapsed time: 00:03:37s. Time for last 1,000,000: 12s. Last read position: NC_059313.1:66,446,868
INFO 2022-08-25 10:14:30 AddOrReplaceReadGroups Processed 18,000,000 records. Elapsed time: 00:03:49s. Time for last 1,000,000: 12s. Last read position: NC_059313.1:103,510,691
INFO 2022-08-25 10:14:43 AddOrReplaceReadGroups Processed 19,000,000 records. Elapsed time: 00:04:02s. Time for last 1,000,000: 12s. Last read position: NC_059314.1:32,977,840
INFO 2022-08-25 10:14:56 AddOrReplaceReadGroups Processed 20,000,000 records. Elapsed time: 00:04:15s. Time for last 1,000,000: 12s. Last read position: NC_059314.1:83,398,361
INFO 2022-08-25 10:15:08 AddOrReplaceReadGroups Processed 21,000,000 records. Elapsed time: 00:04:27s. Time for last 1,000,000: 12s. Last read position: NC_059315.1:28,980,159
INFO 2022-08-25 10:15:21 AddOrReplaceReadGroups Processed 22,000,000 records. Elapsed time: 00:04:40s. Time for last 1,000,000: 12s. Last read position: NC_059315.1:72,355,237
INFO 2022-08-25 10:15:34 AddOrReplaceReadGroups Processed 23,000,000 records. Elapsed time: 00:04:53s. Time for last 1,000,000: 12s. Last read position: NC_059316.1:30,927,882
INFO 2022-08-25 10:15:47 AddOrReplaceReadGroups Processed 24,000,000 records. Elapsed time: 00:05:06s. Time for last 1,000,000: 12s. Last read position: NC_059317.1:6,306,235
INFO 2022-08-25 10:16:00 AddOrReplaceReadGroups Processed 25,000,000 records. Elapsed time: 00:05:19s. Time for last 1,000,000: 12s. Last read position: NC_059317.1:46,365,018
INFO 2022-08-25 10:16:12 AddOrReplaceReadGroups Processed 26,000,000 records. Elapsed time: 00:05:31s. Time for last 1,000,000: 12s. Last read position: NC_059317.1:70,743,720
INFO 2022-08-25 10:16:25 AddOrReplaceReadGroups Processed 27,000,000 records. Elapsed time: 00:05:44s. Time for last 1,000,000: 12s. Last read position: NC_059318.1:50,002,114
INFO 2022-08-25 10:16:37 AddOrReplaceReadGroups Processed 28,000,000 records. Elapsed time: 00:05:56s. Time for last 1,000,000: 12s. Last read position: NC_059318.1:87,467,719
INFO 2022-08-25 10:16:50 AddOrReplaceReadGroups Processed 29,000,000 records. Elapsed time: 00:06:09s. Time for last 1,000,000: 12s. Last read position: NC_059319.1:49,856,896
INFO 2022-08-25 10:17:03 AddOrReplaceReadGroups Processed 30,000,000 records. Elapsed time: 00:06:22s. Time for last 1,000,000: 12s. Last read position: NC_059320.1:15,994,957
INFO 2022-08-25 10:17:16 AddOrReplaceReadGroups Processed 31,000,000 records. Elapsed time: 00:06:35s. Time for last 1,000,000: 13s. Last read position: NC_059320.1:71,486,484
INFO 2022-08-25 10:17:28 AddOrReplaceReadGroups Processed 32,000,000 records. Elapsed time: 00:06:47s. Time for last 1,000,000: 12s. Last read position: NC_059321.1:37,235,293
INFO 2022-08-25 10:17:39 AddOrReplaceReadGroups Processed 33,000,000 records. Elapsed time: 00:06:58s. Time for last 1,000,000: 11s. Last read position: NC_059321.1:37,239,861
INFO 2022-08-25 10:17:51 AddOrReplaceReadGroups Processed 34,000,000 records. Elapsed time: 00:07:10s. Time for last 1,000,000: 11s. Last read position: NC_059321.1:37,252,878
INFO 2022-08-25 10:18:03 AddOrReplaceReadGroups Processed 35,000,000 records. Elapsed time: 00:07:22s. Time for last 1,000,000: 11s. Last read position: NC_059321.1:63,259,580
INFO 2022-08-25 10:18:16 AddOrReplaceReadGroups Processed 36,000,000 records. Elapsed time: 00:07:35s. Time for last 1,000,000: 12s. Last read position: NC_059322.1:41,562,729
INFO 2022-08-25 10:18:28 AddOrReplaceReadGroups Processed 37,000,000 records. Elapsed time: 00:07:47s. Time for last 1,000,000: 12s. Last read position: NC_059323.1:17,851,723
INFO 2022-08-25 10:18:41 AddOrReplaceReadGroups Processed 38,000,000 records. Elapsed time: 00:08:00s. Time for last 1,000,000: 12s. Last read position: NC_048487.1:4,074
INFO 2022-08-25 10:18:53 AddOrReplaceReadGroups Processed 39,000,000 records. Elapsed time: 00:08:12s. Time for last 1,000,000: 12s. Last read position: NC_048487.1:7,944
INFO 2022-08-25 10:19:05 AddOrReplaceReadGroups Processed 40,000,000 records. Elapsed time: 00:08:24s. Time for last 1,000,000: 12s. Last read position: */*
INFO 2022-08-25 10:19:18 AddOrReplaceReadGroups Processed 41,000,000 records. Elapsed time: 00:08:37s. Time for last 1,000,000: 12s. Last read position: */*
INFO 2022-08-25 10:19:31 AddOrReplaceReadGroups Processed 42,000,000 records. Elapsed time: 00:08:50s. Time for last 1,000,000: 12s. Last read position: */*
INFO 2022-08-25 10:19:44 AddOrReplaceReadGroups Processed 43,000,000 records. Elapsed time: 00:09:03s. Time for last 1,000,000: 12s. Last read position: */*
INFO 2022-08-25 10:19:57 AddOrReplaceReadGroups Processed 44,000,000 records. Elapsed time: 00:09:16s. Time for last 1,000,000: 13s. Last read position: */*
INFO 2022-08-25 10:20:10 AddOrReplaceReadGroups Processed 45,000,000 records. Elapsed time: 00:09:29s. Time for last 1,000,000: 13s. Last read position: */*
INFO 2022-08-25 10:20:23 AddOrReplaceReadGroups Processed 46,000,000 records. Elapsed time: 00:09:42s. Time for last 1,000,000: 13s. Last read position: */*
[Thu Aug 25 10:20:27 EDT 2022] picard.sam.AddOrReplaceReadGroups done. Elapsed time: 9.79 minutes.
Runtime.totalMemory()=1512570880
Using GATK jar /ches/home00/lgspeight/gatk-4.2.6.1/gatk-package-4.2.6.1-local.jar
Running:
java -Dsamjdk.use_async_io_read_samtools=false -Dsamjdk.use_async_io_write_samtools=true -Dsamjdk.use_async_io_write_tribble=false -Dsamjdk.compression_level=2 -DGATK_STACKTRACE_ON_USER_EXCEPTION=true -jar /ches/home00/lgspeight/gatk-4.2.6.1/gatk-package-4.2.6.1-local.jar ASEReadCounter -R /ches/data10/lgspeight/RNA-Seq/01_rawdata/genome/Clam_genome.fna -I /ches/data10/lgspeight/results/Align/A_1_32.2.bam -V /ches/data10/lgspeight/results/SNP_Call/SNPS/A_1_32-var.final.vcf.gz -O /ches/data10/lgspeight/results/ASE/A_1_32.table
10:20:31.049 INFO NativeLibraryLoader - Loading libgkl_compression.so from jar:file:/ches/home00/lgspeight/gatk-4.2.6.1/gatk-package-4.2.6.1-local.jar!/com/intel/gkl/native/libgkl_compression.so
10:20:31.259 INFO ASEReadCounter - ------------------------------------------------------------
10:20:31.260 INFO ASEReadCounter - The Genome Analysis Toolkit (GATK) v4.2.6.1
10:20:31.261 INFO ASEReadCounter - For support and documentation go to https://software.broadinstitute.org/gatk/
10:20:31.261 INFO ASEReadCounter - Executing as lgspeight@pm01.hpc.vims.edu on Linux v3.10.0-514.el7.x86_64 amd64
10:20:31.262 INFO ASEReadCounter - Java runtime: OpenJDK 64-Bit Server VM v1.8.0_292-b10
10:20:31.262 INFO ASEReadCounter - Start Date/Time: August 25, 2022 10:20:31 AM EDT
10:20:31.263 INFO ASEReadCounter - ------------------------------------------------------------
10:20:31.263 INFO ASEReadCounter - ------------------------------------------------------------
10:20:31.264 INFO ASEReadCounter - HTSJDK Version: 2.24.1
10:20:31.265 INFO ASEReadCounter - Picard Version: 2.27.1
10:20:31.265 INFO ASEReadCounter - Built for Spark Version: 2.4.5
10:20:31.265 INFO ASEReadCounter - HTSJDK Defaults.COMPRESSION_LEVEL : 2
10:20:31.266 INFO ASEReadCounter - HTSJDK Defaults.USE_ASYNC_IO_READ_FOR_SAMTOOLS : false
10:20:31.266 INFO ASEReadCounter - HTSJDK Defaults.USE_ASYNC_IO_WRITE_FOR_SAMTOOLS : true
10:20:31.266 INFO ASEReadCounter - HTSJDK Defaults.USE_ASYNC_IO_WRITE_FOR_TRIBBLE : false
10:20:31.267 INFO ASEReadCounter - Deflater: IntelDeflater
10:20:31.267 INFO ASEReadCounter - Inflater: IntelInflater
10:20:31.267 INFO ASEReadCounter - GCS max retries/reopens: 20
10:20:31.267 INFO ASEReadCounter - Requester pays: disabled
10:20:31.268 INFO ASEReadCounter - Initializing engine
10:20:32.349 INFO FeatureManager - Using codec VCFCodec to read file file:///ches/data10/lgspeight/results/SNP_Call/SNPS/A_1_32-var.final.vcf.gz
10:20:32.556 INFO ASEReadCounter - Done initializing engine
10:20:32.563 INFO ProgressMeter - Starting traversal
10:20:32.563 INFO ProgressMeter - Current Locus Elapsed Minutes Loci Processed Loci/Minute
10:20:32.694 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:17635
10:20:32.703 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:17649
10:20:32.704 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:17652
10:20:32.715 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:17693
10:20:32.721 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:17738
10:20:32.724 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:17780
10:20:32.726 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:17795
10:20:32.730 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:17823
10:20:32.731 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:17824
10:20:32.731 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:17825
10:20:32.738 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:17848
10:20:32.747 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:17960
10:20:32.747 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:17961
10:20:32.748 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:17962
10:20:32.756 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:53708
10:20:32.757 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:53773
10:20:32.757 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:53774
10:20:32.757 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:53805
10:20:32.758 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:53833
10:20:32.758 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:53885
10:20:32.759 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:53910
10:20:32.759 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:53921
10:20:32.765 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:88233
10:20:32.765 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:88234
10:20:32.765 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:88235
10:20:32.765 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:88236
10:20:32.765 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:88237
10:20:32.765 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:88238
10:20:32.772 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:88594
10:20:32.776 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:89303
10:20:32.784 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:101686
10:20:32.784 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:101692
10:20:32.797 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:175603
10:20:32.798 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:175647
10:20:32.799 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:175710
10:20:32.799 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:175720
10:20:32.831 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:294250
10:20:32.844 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:302438
10:20:32.844 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:302473
10:20:32.844 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:302483
10:20:32.853 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:305782
10:20:32.865 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:307388
10:20:32.893 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:318624
10:20:32.894 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:318663
10:20:32.894 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:318709
10:20:32.896 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:319080
10:20:32.897 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:319189
10:20:32.901 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:320764
10:20:32.968 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:379966
10:20:32.969 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:380374
10:20:33.021 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:409110
10:20:33.037 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:418161
10:20:33.037 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:418177
10:20:33.038 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:418226
10:20:33.038 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:418267
10:20:33.075 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:439003
10:20:33.075 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:439028
10:20:33.076 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:439069
10:20:33.130 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:688150
10:20:33.131 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:737913
10:20:33.132 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:737949
10:20:33.132 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:737965
10:20:33.132 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:738016
10:20:33.133 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:738019
10:20:33.137 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:880879
10:20:33.137 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:880883
10:20:33.138 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:880965
10:20:33.138 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:880981
10:20:33.138 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:881011
10:20:33.191 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:972414
10:20:33.192 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:972723
10:20:33.221 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1075252
10:20:33.221 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1075253
10:20:33.221 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1075254
10:20:33.227 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1079125
10:20:33.228 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1079171
10:20:33.232 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1080550
10:20:33.257 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1107648
10:20:33.259 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1111312
10:20:33.260 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1111462
10:20:33.260 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1111475
10:20:33.262 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1115182
10:20:33.263 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1115299
10:20:33.264 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1115337
10:20:33.264 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1115346
10:20:33.286 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1128753
10:20:33.286 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1128825
10:20:33.286 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1128870
10:20:33.286 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1128885
10:20:33.288 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1129075
10:20:33.289 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1129215
10:20:33.289 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1129232
10:20:33.289 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1129323
10:20:33.290 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1129461
10:20:33.294 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1129876
10:20:33.296 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1130129
10:20:33.297 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1130229
10:20:33.299 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1130371
10:20:33.299 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1130414
10:20:33.300 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1130415
10:20:33.300 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1130416
10:20:33.300 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1130417
10:20:33.300 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1130418
10:20:33.301 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1130419
10:20:33.301 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1130420
10:20:33.305 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1130654
10:20:33.305 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1130664
10:20:33.307 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1130924
10:20:33.308 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1131058
10:20:33.308 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1131059
10:20:33.308 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1131060
10:20:33.308 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1131061
10:20:33.309 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1131062
10:20:33.309 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1131134
10:20:33.309 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1131141
10:20:33.312 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1131352
10:20:33.313 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1131390
10:20:33.320 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1132144
10:20:33.320 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1132166
10:20:33.321 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1132331
10:20:33.322 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1132384
10:20:33.323 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1132496
10:20:33.326 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1132535
10:20:33.327 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1132565
10:20:33.327 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1132626
10:20:33.327 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1132632
10:20:33.328 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1132644
10:20:33.328 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1132647
10:20:33.329 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1132748
10:20:33.329 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1132769
10:20:33.330 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1132840
10:20:33.330 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1132841
10:20:33.330 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1132842
10:20:33.330 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1132864
10:20:33.332 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1132948
10:20:33.333 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1133004
10:20:33.335 WARN ASEReadCounter - Ignoring site: cannot run ASE on non-biallelic sites: [VC Unknown @ NC_059305.1:1133075 Q192.71 of type=SNP alleles=[G*, A, T] attr={AC=[1, 1], AN=2, DP=17, DP4=[0, 0, 8, 9], FS=0, MQ=60, MQ0F=0, MQSBZ=0, SGB=-0.690438, VDB=0.0134843} GT=GT:PL 1/2:255,174,144,227,0,206 filters=
10:20:33.337 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1133167
10:20:33.343 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1133643
10:20:33.343 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1133654
10:20:33.344 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1133662
10:20:33.344 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1133732
10:20:33.344 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1133733
10:20:33.347 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1133861
10:20:33.348 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1133880
10:20:33.348 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1133917
10:20:33.350 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1134154
10:20:33.351 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1134155
10:20:33.353 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1134723
10:20:33.372 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1142318
10:20:33.372 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1142334
10:20:33.384 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1149057
10:20:33.401 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1159542
10:20:33.402 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1159543
10:20:33.402 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1159544
10:20:33.402 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1159545
10:20:33.402 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1159546
10:20:33.402 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1159547
10:20:33.402 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1159548
10:20:33.402 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1159549
10:20:33.402 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1159550
10:20:33.402 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1159551
10:20:33.402 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1159552
10:20:33.402 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1159553
10:20:33.416 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1166830
10:20:33.416 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1166832
10:20:33.416 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1166876
10:20:33.416 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1166899
10:20:33.416 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1166900
10:20:33.448 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1184700
10:20:33.462 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1193261
10:20:33.463 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1193299
10:20:33.463 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1193320
10:20:33.463 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1193418
10:20:33.464 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1193419
10:20:33.471 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1197507
10:20:33.494 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1209456
10:20:33.496 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1209635
10:20:33.497 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1209869
10:20:33.497 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1209881
10:20:33.497 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1209897
10:20:33.497 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1209970
10:20:33.498 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1210021
10:20:33.498 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1210112
10:20:33.536 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1238784
10:20:33.536 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1238792
10:20:33.544 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1242931
10:20:33.548 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1246421
10:20:33.550 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1247865
10:20:33.565 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1259312
10:20:33.565 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1259408
10:20:33.567 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1260566
10:20:33.568 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1260626
10:20:33.568 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1260731
10:20:33.568 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1260820
10:20:33.588 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1282891
10:20:33.588 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1282892
10:20:33.588 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1282893
10:20:33.588 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1282894
10:20:33.588 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1282895
10:20:33.588 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1282896
10:20:33.588 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1282897
10:20:33.589 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1283441
10:20:33.590 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1283549
10:20:33.590 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1283729
10:20:33.590 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1283730
10:20:33.590 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1283731
10:20:33.598 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1294653
10:20:33.598 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1294654
10:20:33.598 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1294655
10:20:33.602 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1297407
10:20:33.602 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1297543
10:20:33.614 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1308658
10:20:33.620 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1314907
10:20:33.621 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1314947
10:20:33.621 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1315006
10:20:33.622 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1315248
10:20:33.622 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1315319
10:20:33.622 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1315386
10:20:33.622 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1315387
10:20:33.623 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1315388
10:20:33.625 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1317140
10:20:33.626 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1317918
10:20:33.627 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1318027
10:20:33.627 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1318028
10:20:33.627 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1318029
10:20:33.627 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1318030
10:20:33.627 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1318031
10:20:33.634 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1322084
10:20:33.634 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1322085
10:20:33.634 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1322086
10:20:33.634 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1322087
10:20:33.634 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1322088
10:20:33.635 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1322089
10:20:33.635 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1322238
10:20:33.643 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1327415
10:20:33.643 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1327477
10:20:33.644 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1327553
10:20:33.644 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1327567
10:20:33.645 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1327584
10:20:33.645 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1327598
10:20:33.660 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1337641
10:20:33.675 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1349108
10:20:33.675 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1349109
10:20:33.675 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1349110
10:20:33.675 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1349111
10:20:33.675 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1349112
10:20:33.700 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1371000
10:20:33.700 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1371019
10:20:33.700 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1371021
10:20:33.700 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1371142
10:20:33.700 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1371143
10:20:33.701 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1371144
10:20:33.701 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1371145
10:20:33.701 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1371146
10:20:33.701 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1371147
10:20:33.701 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1371148
10:20:33.701 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1371167
10:20:33.702 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1371467
10:20:33.702 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1371489
10:20:33.702 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1371548
10:20:33.703 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1371558
10:20:33.703 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1371602
10:20:33.704 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1371622
10:20:33.705 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1371773
10:20:33.712 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1377277
10:20:33.712 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1377278
10:20:33.712 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1377304
10:20:33.713 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1377322
10:20:33.715 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1379021
10:20:33.715 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1379095
10:20:33.739 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1404569
10:20:33.740 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1404570
10:20:33.740 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1404571
10:20:33.740 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1404572
10:20:33.740 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1404573
10:20:33.740 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1404574
10:20:33.740 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1404575
10:20:33.740 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1404576
10:20:33.749 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1416464
10:20:33.772 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1445035
10:20:33.785 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1463588
10:20:33.785 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1463592
10:20:33.786 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1464450
10:20:33.786 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1464451
10:20:33.786 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1464452
10:20:33.786 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1464474
10:20:33.786 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1464582
10:20:33.789 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1466820
10:20:33.789 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1466856
10:20:33.789 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1466998
10:20:33.789 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1467015
10:20:33.789 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1467080
10:20:33.789 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1467084
10:20:33.790 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1467085
10:20:33.798 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1477802
10:20:33.798 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1477835
10:20:33.799 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1478043
10:20:33.799 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1478061
10:20:33.800 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1478309
10:20:33.800 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1478327
10:20:33.801 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1478345
10:20:33.801 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1478363
10:20:33.801 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1478397
10:20:33.803 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1478809
10:20:33.805 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1479593
10:20:33.805 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1479606
10:20:33.805 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1479607
10:20:33.805 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1479608
10:20:33.805 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1479609
10:20:33.805 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1479610
10:20:33.805 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1479611
10:20:33.805 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1479612
10:20:33.805 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1479744
10:20:33.806 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1479771
10:20:33.807 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1481505
10:20:33.808 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1481577
10:20:33.808 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1481605
10:20:33.808 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1481606
10:20:33.808 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1481607
10:20:33.808 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1481608
10:20:33.808 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1481609
10:20:33.808 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1481610
10:20:33.808 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1481611
10:20:33.808 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1481612
10:20:33.809 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1481613
10:20:33.809 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1481614
10:20:33.814 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1486738
10:20:33.814 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1486823
10:20:33.815 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1486946
10:20:33.818 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1491300
10:20:33.819 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1491323
10:20:33.819 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1491366
10:20:33.819 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1491367
10:20:33.819 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1491368
10:20:33.819 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1491369
10:20:33.819 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1491370
10:20:33.824 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1496285
10:20:33.825 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1496445
10:20:33.825 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1496462
10:20:33.828 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1497078
10:20:33.835 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1499249
10:20:33.836 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1499277
10:20:33.837 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1499627
10:20:33.839 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1500025
10:20:33.840 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1500148
10:20:33.840 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1500218
10:20:33.841 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1500308
10:20:33.841 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1500318
10:20:33.841 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1500359
10:20:33.841 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1500360
10:20:33.842 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1500361
10:20:33.844 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1500655
10:20:33.845 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1500844
10:20:33.845 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1500847
10:20:33.852 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1504634
10:20:33.859 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1511057
10:20:33.862 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1512728
10:20:33.862 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1512735
10:20:33.862 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1512745
10:20:33.870 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1524651
10:20:33.876 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1529642
10:20:33.877 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1529811
10:20:33.878 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1531000
10:20:33.909 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1555728
10:20:33.910 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1555992
10:20:33.914 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1557410
10:20:33.914 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1557614
10:20:33.914 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1557615
10:20:33.914 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1557616
10:20:33.914 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1557617
10:20:33.914 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1557618
10:20:33.914 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1557619
10:20:33.915 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1557620
10:20:33.915 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1557621
10:20:33.915 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1557622
10:20:33.915 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1557623
10:20:33.915 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1557624
10:20:33.931 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1574784
10:20:33.932 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1574855
10:20:33.932 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1574911
10:20:33.934 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1576841
10:20:33.934 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1576842
10:20:33.935 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1576843
10:20:33.935 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1576844
10:20:33.937 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1579679
10:20:33.938 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1579882
10:20:33.938 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1579883
10:20:33.938 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1579884
10:20:33.938 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1579885
10:20:33.938 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1579886
10:20:33.938 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1579887
10:20:33.938 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1579888
10:20:33.938 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1579942
10:20:33.946 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1589158
10:20:33.946 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1589278
10:20:33.951 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1595590
10:20:33.954 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1617584
10:20:33.965 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1630285
10:20:33.965 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1630289
10:20:34.000 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1656168
10:20:34.010 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1656957
10:20:34.010 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1656958
10:20:34.010 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1656959
10:20:34.036 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1661938
10:20:34.039 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1662523
10:20:34.039 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1662524
10:20:34.043 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1663063
10:20:34.071 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1672551
10:20:34.082 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1678047
10:20:34.083 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1678220
10:20:34.083 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1678251
10:20:34.092 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1682194
10:20:34.092 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1682195
10:20:34.092 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1682232
10:20:34.092 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1682438
10:20:34.106 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1691441
10:20:34.106 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1691484
10:20:34.106 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1691485
10:20:34.107 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1691608
10:20:34.111 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1694219
10:20:34.112 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1694401
10:20:34.112 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1694537
10:20:34.113 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1694700
10:20:34.113 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1694749
10:20:34.113 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1694775
10:20:34.113 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1694786
10:20:34.113 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1694904
10:20:34.114 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1695138
10:20:34.114 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1695139
10:20:34.115 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1695140
10:20:34.115 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1695141
10:20:34.115 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1695142
10:20:34.115 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1695143
10:20:34.115 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1695193
10:20:34.115 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1695215
10:20:34.115 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1695277
10:20:34.115 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1695335
10:20:34.115 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1695336
10:20:34.115 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1695337
10:20:34.115 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1695338
10:20:34.115 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1695339
10:20:34.116 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1695340
10:20:34.132 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1706939
10:20:34.145 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1719275
10:20:34.145 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1719320
10:20:34.166 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1734784
10:20:34.174 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1758388
10:20:34.175 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1774396
10:20:34.175 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1774404
10:20:34.181 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1776046
10:20:34.182 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1776097
10:20:34.183 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1776162
10:20:34.190 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1776730
10:20:34.196 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1777190
10:20:34.197 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1777209
10:20:34.217 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1778349
10:20:34.222 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1778740
10:20:34.223 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1778804
10:20:34.224 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1778919
10:20:34.226 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1779035
10:20:34.226 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1779079
10:20:34.227 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1779150
10:20:34.300 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1788649
10:20:34.406 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1799377
10:20:34.416 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1802877
10:20:34.417 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1803175
10:20:34.473 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1933651
10:20:34.474 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1933913
10:20:34.474 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1933929
10:20:34.474 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1934026
10:20:34.484 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1945299
10:20:34.484 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1945308
10:20:34.484 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1945320
10:20:34.485 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1945385
10:20:34.492 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1954088
10:20:34.492 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1954120
10:20:34.492 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1954135
10:20:34.500 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1963657
10:20:34.500 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1963717
10:20:34.505 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1967924
10:20:34.509 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1970068
10:20:34.510 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1970289
10:20:34.511 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1970632
10:20:34.511 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1970633
10:20:34.511 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1970837
10:20:34.512 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:1970946
10:20:34.560 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2051148
10:20:34.562 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2096575
10:20:34.563 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2096878
10:20:34.568 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2099108
10:20:34.569 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2099391
10:20:34.570 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2099570
10:20:34.572 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2099929
10:20:34.572 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2100114
10:20:34.573 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2100973
10:20:34.575 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2103692
10:20:34.575 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2103693
10:20:34.575 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2103694
10:20:34.575 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2103695
10:20:34.575 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2103696
10:20:34.576 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2103821
10:20:34.579 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2109519
10:20:34.579 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2109520
10:20:34.579 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2109521
10:20:34.579 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2109522
10:20:34.580 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2110480
10:20:34.580 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2111528
10:20:34.580 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2111562
10:20:34.586 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2121400
10:20:34.586 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2121418
10:20:34.586 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2121444
10:20:34.586 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2121450
10:20:34.587 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2122609
10:20:34.587 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2122618
10:20:34.598 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2135877
10:20:34.654 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2336222
10:20:34.656 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2341758
10:20:34.656 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2341868
10:20:34.657 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2341896
10:20:34.657 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2341978
10:20:34.658 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2342040
10:20:34.659 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2342174
10:20:34.659 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2342175
10:20:34.659 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2342176
10:20:34.663 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2342693
10:20:34.664 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2342737
10:20:34.664 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2342744
10:20:34.664 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2342755
10:20:34.664 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2342763
10:20:34.665 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2342813
10:20:34.667 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2343039
10:20:34.667 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2343066
10:20:34.667 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2343083
10:20:34.667 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2343100
10:20:34.667 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2343122
10:20:34.668 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2343191
10:20:34.669 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2343288
10:20:34.669 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2343326
10:20:34.669 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2343327
10:20:34.669 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2343348
10:20:34.669 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2343368
10:20:34.669 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2343523
10:20:34.670 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2343538
10:20:34.670 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2343545
10:20:34.670 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2343627
10:20:34.670 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2343653
10:20:34.670 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2343665
10:20:34.670 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2343674
10:20:34.670 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2343679
10:20:34.670 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2343689
10:20:34.671 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2343777
10:20:34.671 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2343810
10:20:34.671 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2343837
10:20:34.672 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2344523
10:20:34.672 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2344524
10:20:34.672 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2344525
10:20:34.672 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2344548
10:20:34.673 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2344608
10:20:34.673 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2344609
10:20:34.673 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2344668
10:20:34.673 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2344669
10:20:34.673 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2344670
10:20:34.673 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2344671
10:20:34.673 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2344672
10:20:34.673 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2344673
10:20:34.673 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2344674
10:20:34.673 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2344675
10:20:34.673 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2344676
10:20:34.673 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2344701
10:20:34.673 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2344714
10:20:34.673 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2344720
10:20:34.674 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2344799
10:20:34.676 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2345076
10:20:34.677 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2345205
10:20:34.677 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2345206
10:20:34.677 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2345207
10:20:34.677 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2345208
10:20:34.677 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2345209
10:20:34.677 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2345331
10:20:34.678 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2345532
10:20:34.680 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2346130
10:20:34.680 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2346131
10:20:34.680 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2346132
10:20:34.680 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2346133
10:20:34.680 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2346134
10:20:34.681 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2346135
10:20:34.681 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2346150
10:20:34.681 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2346151
10:20:34.681 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2346225
10:20:34.682 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2346700
10:20:34.682 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2346709
10:20:34.683 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2346746
10:20:34.683 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2346775
10:20:34.683 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2346799
10:20:34.683 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2346890
10:20:34.683 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2346891
10:20:34.683 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2346939
10:20:34.686 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2348089
10:20:34.686 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2348090
10:20:34.686 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2348095
10:20:34.686 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2348176
10:20:34.687 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2348408
10:20:34.687 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2348481
10:20:34.687 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2348488
10:20:34.690 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2349825
10:20:34.693 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2351043
10:20:34.702 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2356781
10:20:34.702 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2356782
10:20:34.702 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2356783
10:20:34.702 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2356784
10:20:34.710 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2362049
10:20:34.711 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2362448
10:20:34.713 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2364138
10:20:34.715 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2364629
10:20:34.719 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2367161
10:20:34.719 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2367170
10:20:34.719 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2367173
10:20:34.720 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2367176
10:20:34.720 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2367189
10:20:34.732 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2375915
10:20:34.732 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2375941
10:20:34.732 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2375942
10:20:34.733 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2376261
10:20:34.733 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2376269
10:20:34.733 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2376329
10:20:34.736 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2377459
10:20:34.739 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2378384
10:20:34.739 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2378453
10:20:34.741 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2379403
10:20:34.742 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2379872
10:20:34.756 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2389163
10:20:34.756 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2389519
10:20:34.758 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2389820
10:20:34.758 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2389826
10:20:34.758 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2389853
10:20:34.758 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2389867
10:20:34.761 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2390982
10:20:34.772 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2402155
10:20:34.779 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2403747
10:20:34.780 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2403955
10:20:34.784 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2405540
10:20:34.784 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2405569
10:20:34.784 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2405570
10:20:34.784 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2405641
10:20:34.784 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2405642
10:20:34.784 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2405643
10:20:34.784 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2405644
10:20:34.784 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2405645
10:20:34.784 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2405646
10:20:34.784 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2405647
10:20:34.784 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2405870
10:20:34.784 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2405907
10:20:34.785 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2406138
10:20:34.789 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2407857
10:20:34.792 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2409314
10:20:34.793 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2410007
10:20:34.793 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2410008
10:20:34.793 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2410009
10:20:34.793 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2410010
10:20:34.793 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2410011
10:20:34.793 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2410012
10:20:34.793 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2410013
10:20:34.793 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2410025
10:20:34.793 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2410158
10:20:34.793 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2410254
10:20:34.793 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2410255
10:20:34.793 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2410286
10:20:34.794 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2410614
10:20:34.798 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2416505
10:20:34.826 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2462559
10:20:34.826 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2462587
10:20:34.826 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2462623
10:20:34.837 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2512904
10:20:34.837 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2512932
10:20:34.837 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2515769
10:20:34.839 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2517733
10:20:34.845 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2525506
10:20:34.846 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2525554
10:20:34.846 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2525665
10:20:34.846 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2526038
10:20:34.846 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2526074
10:20:34.846 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2526089
10:20:34.846 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2526213
10:20:34.846 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2526219
10:20:34.846 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2526333
10:20:34.847 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2526886
10:20:34.847 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2526925
10:20:34.847 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2527642
10:20:34.847 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2527657
10:20:34.847 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2527659
10:20:34.847 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2527706
10:20:34.848 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2533287
10:20:34.857 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2534581
10:20:34.874 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2534982
10:20:34.886 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2535320
10:20:34.987 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2539935
10:20:35.024 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2542130
10:20:35.024 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2542132
10:20:35.024 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2542133
10:20:35.030 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2544469
10:20:35.030 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2544490
10:20:35.031 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2571628
10:20:35.031 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2571629
10:20:35.031 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2571630
10:20:35.031 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2571631
10:20:35.031 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2571632
10:20:35.032 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2571633
10:20:35.032 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2576115
10:20:35.032 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2576116
10:20:35.032 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2576117
10:20:35.032 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2576118
10:20:35.032 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2576119
10:20:35.032 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2576120
10:20:35.032 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2576121
10:20:35.032 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2576122
10:20:35.032 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2576123
10:20:38.483 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2662286
10:20:38.508 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2662398
10:20:38.522 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2662466
10:20:39.383 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2666549
10:20:43.257 INFO ProgressMeter - NC_059305.1:2682358 0.2 238000 1335453.1
10:20:43.722 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2700027
10:20:43.723 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2700101
10:20:43.723 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2700102
10:20:43.723 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2700103
10:20:43.723 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2700104
10:20:43.723 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2700105
10:20:43.723 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2700106
10:20:43.723 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2700107
10:20:43.723 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2700108
10:20:43.723 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2700109
10:20:43.723 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2700139
10:20:43.723 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2700236
10:20:43.724 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2700267
10:20:43.724 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2700306
10:20:43.725 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2700389
10:20:43.725 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2700398
10:20:43.725 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2700482
10:20:43.744 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2709917
10:20:43.779 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2723346
10:20:43.784 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2725521
10:20:43.785 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2725608
10:20:43.788 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2725953
10:20:43.789 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2726068
10:20:43.789 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2726255
10:20:43.789 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2726341
10:20:43.789 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2726430
10:20:43.790 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2726447
10:20:43.791 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2727556
10:20:43.791 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2727578
10:20:43.791 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2727611
10:20:43.791 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2727690
10:20:43.791 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2727720
10:20:43.791 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2727776
10:20:43.791 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2727779
10:20:43.795 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2733001
10:20:43.795 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2733008
10:20:43.795 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2733011
10:20:43.796 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2733138
10:20:43.803 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2734578
10:20:43.912 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2759043
10:20:43.933 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2768045
10:20:43.933 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2768046
10:20:43.933 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2768047
10:20:43.942 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2770218
10:20:43.942 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2770299
10:20:43.953 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2772553
10:20:43.960 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2775405
10:20:43.960 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2775412
10:20:43.963 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2780686
10:20:43.964 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2780773
10:20:43.965 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2781402
10:20:43.965 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2781495
10:20:43.965 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2781546
10:20:43.967 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2782911
10:20:43.967 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2782920
10:20:43.967 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2782944
10:20:43.967 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2782983
10:20:43.967 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2783007
10:20:43.968 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2783604
10:20:43.968 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2783699
10:20:43.968 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2783726
10:20:43.971 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2784990
10:20:43.975 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2788342
10:20:43.975 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2788366
10:20:43.975 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2788450
10:20:43.975 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2788451
10:20:43.975 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2788452
10:20:43.981 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2793671
10:20:43.981 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2793711
10:20:43.982 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2793840
10:20:43.982 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2793842
10:20:43.982 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2793864
10:20:43.982 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2793895
10:20:43.982 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2793908
10:20:43.982 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2794038
10:20:43.982 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2794075
10:20:43.982 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2794079
10:20:43.982 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2794110
10:20:43.982 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2794165
10:20:43.982 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2794192
10:20:43.982 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2794197
10:20:43.983 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2794220
10:20:43.983 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2794262
10:20:43.984 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2794674
10:20:43.984 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2794675
10:20:43.984 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2794676
10:20:43.984 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2794677
10:20:43.984 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2794678
10:20:43.984 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2794679
10:20:43.984 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2794680
10:20:43.984 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2794691
10:20:43.986 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2795029
10:20:43.990 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2795381
10:20:43.991 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2795521
10:20:43.991 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2795522
10:20:43.991 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2795563
10:20:43.991 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2795773
10:20:43.991 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2795789
10:20:43.992 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2795905
10:20:43.992 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2795906
10:20:43.992 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2795907
10:20:43.992 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2795908
10:20:43.992 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2795909
10:20:43.992 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2795910
10:20:43.992 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2795911
10:20:43.992 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2795912
10:20:43.992 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2795967
10:20:43.994 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2796831
10:20:43.994 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2796832
10:20:43.994 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2796833
10:20:43.994 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2796834
10:20:43.995 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2796979
10:20:43.996 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2818275
10:20:44.001 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2821619
10:20:44.001 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2821643
10:20:44.001 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2821644
10:20:44.001 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2821652
10:20:44.017 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2840611
10:20:44.017 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2840654
10:20:44.017 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2840679
10:20:44.017 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2840698
10:20:44.017 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2840739
10:20:44.017 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2840746
10:20:44.017 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2840841
10:20:44.017 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2840844
10:20:44.044 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2870240
10:20:44.044 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2870300
10:20:44.051 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2878153
10:20:44.056 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2881566
10:20:44.056 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2881571
10:20:44.056 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2881600
10:20:44.056 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2881727
10:20:44.073 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2901122
10:20:44.074 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2902004
10:20:44.075 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2902739
10:20:44.076 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2903194
10:20:44.076 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2903200
10:20:44.076 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2903201
10:20:44.076 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2903202
10:20:44.076 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2903375
10:20:44.076 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2903436
10:20:44.076 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2903495
10:20:44.077 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2903665
10:20:44.077 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2903716
10:20:44.078 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2903910
10:20:44.079 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2904244
10:20:44.079 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2904375
10:20:44.079 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2904401
10:20:44.079 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2904506
10:20:44.079 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2904507
10:20:44.079 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2904508
10:20:44.079 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2904509
10:20:44.079 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2904525
10:20:44.079 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2904636
10:20:44.079 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2904642
10:20:44.080 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2904687
10:20:44.080 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2904891
10:20:44.081 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2904948
10:20:44.081 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2904955
10:20:44.081 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2905674
10:20:44.082 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2905826
10:20:44.082 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2905827
10:20:44.082 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2905835
10:20:44.082 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2905843
10:20:44.082 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2905846
10:20:44.082 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2905993
10:20:44.082 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2906114
10:20:44.083 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2906233
10:20:44.083 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2906283
10:20:44.083 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2906316
10:20:44.083 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2906368
10:20:44.083 WARN ASEReadCounter - Ignoring site: variant is not het at postion: NC_059305.1:2906369
10:20:44.086 INFO ASEReadCounter - Shutting down engine
[August 25, 2022 10:20:44 AM EDT] org.broadinstitute.hellbender.tools.walkers.rnaseq.ASEReadCounter done. Elapsed time: 0.22 minutes.
Runtime.totalMemory()=2632450048
***********************************************************************
A USER ERROR has occurred: More then one variant context at position: NC_059305.1:2906370
***********************************************************************
org.broadinstitute.hellbender.exceptions.UserException: More then one variant context at position: NC_059305.1:2906370
at org.broadinstitute.hellbender.tools.walkers.rnaseq.ASEReadCounter.apply(ASEReadCounter.java:193)
at org.broadinstitute.hellbender.engine.LocusWalker.lambda$traverse$0(LocusWalker.java:154)
at java.util.Iterator.forEachRemaining(Iterator.java:116)
at org.broadinstitute.hellbender.engine.LocusWalker.traverse(LocusWalker.java:152)
at org.broadinstitute.hellbender.engine.GATKTool.doWork(GATKTool.java:1085)
at org.broadinstitute.hellbender.cmdline.CommandLineProgram.runTool(CommandLineProgram.java:140)
at org.broadinstitute.hellbender.cmdline.CommandLineProgram.instanceMainPostParseArgs(CommandLineProgram.java:192)
at org.broadinstitute.hellbender.cmdline.CommandLineProgram.instanceMain(CommandLineProgram.java:211)
at org.broadinstitute.hellbender.Main.runCommandLineProgram(Main.java:160)
at org.broadinstitute.hellbender.Main.mainEntry(Main.java:203)
at org.broadinstitute.hellbender.Main.main(Main.java:289)
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I couldnt add this to my post because I had reached the max number of characters.
d) File output (head and tail of one file)
contig position variantID refAllele altAllele refCounaltCount totalCount lowMAPQDepth lowBaseQDepth rawDepth otherBases improperPairs
NC_059305.1 88554 . C T 1 2 3 0 0
NC_059305.1 306126 . G A 6 3 9 0 0
NC_059305.1 306130 . C T 5 3 8 0 0
NC_059305.1 318568 . C T 3 5 8 0 17 0 9
NC_059305.1 379937 . C T 3 2 5 0 0
NC_059305.1 1098226 . G T 1 2 3 0 0
NC_059305.1 1098246 . A G 1 2 3 0 0
NC_059305.1 1100651 . A G 1 3 4 0 0
NC_059305.1 1129066 . C T 3 3 6 0 1
.
.
.
NC_059305.1 2903861 . A G 1 1 2 0 1
NC_059305.1 2903985 . A G 2 3 5 0 1
NC_059305.1 2904143 . T C 8 4 12 0 13 0 1
NC_059305.1 2904663 . A G 1 4 5 0 3
NC_059305.1 2904681 . A G 1 4 5 0 3
NC_059305.1 2904730 . T C 5 4 9 0 10 0 1
NC_059305.1 2904743 . A G 5 4 9 0 10 0 1
NC_059305.1 2904792 . C G 4 4 8 0 12 0 4
NC_059305.1 2904796 . C T 4 4 8 0 12 0 4
NC_059305.1 2904797 . A T 4 4 8 0 12 0 4
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Hi Leslie Youtsey,
Thank you again for writing to the GATK forum! Let’s see how we can solve this.
I identified a seemingly identical issue that occurred to another user last year. They also were getting the “more than one variant context at position” error message when running ASWReadCounter. If you scroll to the bottom of this GitHub thread, bw2 commented with a potential solution.
The error seems to be caused by the input VCF “having two SNPs on separate rows but with the same position… or by an INDEL that overlaps a SNP”.
They suggest first filtering out indels and multiallelic sites before running ASEReadCounter. Since it seems to have led them to success, I suggest first trying this out.
The command they included to filter out the indels and multiallelic sites is as follows:
gatk SelectVariants -R hg38.fa. --variant dna_variants.vcf.bgz --restrict-alleles-to BIALLELIC -select 'vc.getHetCount()==1' --select-type-to-include SNP -O dna_variants.selected.vcf.bgz
bcftools norm --rm-dup all dna_variants.selected.vcf.bgz | bgzip > out.vcf.gzI hope this helps! Please let me know if this leads you to success. If you have any questions in the meantime, please do not hesitate to reach back out.
Best,
Anthony -
Hi Leslie Youtsey,
We haven’t heard from you in a while so we will be closing out your ticket in our system. If you still require assistance, you need only respond to this thread, and we’ll create a follow-up ticket to pick up where we left off.
Thank you again for contributing to our GATK forum!
Best,
Anthony
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