Genome Analysis Toolkit

Variant Discovery in High-Throughput Sequencing Data

GATK process banner

Need Help?

Search our documentation

Community Forum

Hi, How can we help?

Developed in the Data Sciences Platform at the Broad Institute, the toolkit offers a wide variety of tools with a primary focus on variant discovery and genotyping. Its powerful processing engine and high-performance computing features make it capable of taking on projects of any size. Learn more

"main" java.lang.OutOfMemoryError: Requested array size exceeds VM limit

Answered
0

4 comments

  • Avatar
    Genevieve Brandt (she/her)

    Hello,

    Do you have 2800G memory available on your machine? It looks like your error might be coming from GATK trying to use 2800G but it is not available.

    Best,

    Genevieve

    0
    Comment actions Permalink
  • Avatar
    蒲鹏

    Hi,

    I figured out that it was caused by wrong interval_list input. it should be snp-only.interval_list, but I didn't find any online file.

    I'm working on generating it by myself. Do you know where I can find it?

    thanks!

    PENG PU

    0
    Comment actions Permalink
  • Avatar
    蒲鹏

    Hi,@Genevieve Brandt

    Problem solved!

    First, I slected snp-only.vcf from gnomad.genomes.r2.1.sites.liftoverToHg38.INFO_ANNOTATIONS_FIXED.vcf.gz using SelectVariants, and got interval_list by IntervalListTools.

    Just in case some friends do have same issue.

    Best,

    PENG PU

    0
    Comment actions Permalink
  • Avatar
    Genevieve Brandt (she/her)

    Thank you for posting your solution PENG PU! I'm glad you have solved your problem.

    0
    Comment actions Permalink

Please sign in to leave a comment.

Powered by Zendesk