I have what i believe to be unstranded RNA data, and when I ran CollectRnaseqMetrics with
I got absolutely no strandedness metrics, which I would need to confirm that my sample is indeed unstranded. is there any way to force reporting of certain metrics, such as `NUM_R1_TRANSCRIPT_STRAND_READS` and `NUM_R2_TRANSCRIPT_STRAND_READS` even when unstranded? I could obviously use the `FIRST_READ_TRANSCRIPTION_STRAND`, but i want to avoid incorrectly reporting `CORRECT_STRAND_READS`, etc.
Please sign in to leave a comment.