We have been following tutorial(s) 11682 and 11683 for sensitive detection of copy ratio alterations in benign tissue utilizing the PON sensitivity in place of a matched normal when one may not be easily accessible. Currently, we have a PON of 47 germlines generated by "CreateReadCountPanelOfNormals".
Although I have read in many posts that a range between 40-100 specimen is good. How and/or is there a way to determine the optimum number of samples to include in the PON? We have the ability to potentially add several hundred to our panel but are not sure at what point this becomes excessive (both financially and temporally).
Thank you in advance for your help
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