Since I had problems with some BAM files, I ran ValidateSamFile to checked them and I got the Follow error:
ERROR::INVALID_UNALIGNED_MATE_START:Record 7208324, Read name ST-E00318:811:H2YNGCCX2:2:2204:25296:14195, The unaligned mate start position is 154003504, should be 0
However, I understand what the problem is, but I can't find how to fix it.
P.D. I have tried CleanSam and FixMateInformation but it hasn't worked for me
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