Funcotator missing AF
I am using Funcotator on GATK4.
I found the the following particular variant in vcf file has missing AF value in the maf file produced by Funcotator.
2113 variants out of 1032388 total variants are having missing AF after Funcotator annotation.
Could anyone please help me?
chr3 14145949 . G T 187 PASS
SAMPLE=Horizon-CMP001;TYPE=SNV;DP=458;VD=53;AF=0.1157;BIAS=2:2;REFBIAS=245:160;VARBIAS=33:20;PMEAN=23.5;PSTD=1;QUAL=32.7;QSTD=1;SBF=0.88159;ODDRATIO=1.07738884040639;MQ=60;SN=9.6;HIAF=0.1116;ADJAF=0.0044;SHIFT3=0;MSI=1;MSILEN=1;NM=1.1;HICNT=48;HICOV=430;LSEQ=TGGGCGCTCAGCTCACAGCT;RSEQ=CTCAAATGGGAACAGGTGGG;DUPRATE=0;SPLITREAD=0;SPANPAIR=0
GT:DP:VD:AD:AF:RD:ALD 0/1:458:53:405,53:0.1157:245,160:33,20
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Hello Haiying Kong,
We need more information to look into this issue, please make sure to include the GATK version and your Funcotator command.
More information about how to write a post is here: https://gatk.broadinstitute.org/hc/en-us/articles/360053424571-How-to-Write-a-Post
Thank you,
Genevieve
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