MarkDuplicates
I have sorted my bam file by query name. what should be the --ASSUME_SORTED option in MarkDuplicates.
In manual it is written about coordinate sorted bam file but not about query name sorted bam file.
Kindly give suggestion.
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Hi JAY SINGH,
Please see this tool documentation page for MarkDuplicates. --ASSUME_SORTED is deprecated, --ASSUME_SORT_ORDER is a better option to use.
There is also a good explanation of the sort at the top of the page.
Hope this helps!
Genevieve
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