Funcotator Errors : I/O error in the advisory file locking logic (disk I/O error)
Hi GATK Team
I'm having trouble using Funcotator to annotate somatic vcf files.
I ran GATK (v4.1.9.0) Funcotator using the command below.
gatk Funcotator \
--variant Somatic.vcf.gz \
--reference genome.fa \
--ref-version hg19 \
--data-sources-path funcotator_dataSources.v1.6.20190124s \
--output Somatic.merge.funcotated.maf \
--output-file-format MAF
However, this error message popped up.
Error Message:
Using GATK jar /mnt/ruby/Users/uugi0620/phenomata/Anaconda3/envs/gatk4/share/gatk4-4.1.9.0-0/gatk-package-4.1.9.0-local.jar
Running:
java -Dsamjdk.use_async_io_read_samtools=false -Dsamjdk.use_async_io_write_samtools=true -Dsamjdk.use_async_io_write_tribble=false -Dsamjdk.compression_level=2 -jar /mnt/ruby/Users/uugi0620/phenomata/Anaconda3/envs/gatk4/share/gatk4-4.1.9.0-0/gatk-package-4.1.9.0-local.jar Funcotator --variant 20004_T.somatic.merge.vcf.gz --reference /clinix1/Analysis/mongol/phenomata/Reference/PCAWG_reference/bwa-mem/genome.fa --ref-version hg19 --data-sources-path /clinix1/Analysis/mongol/phenomata/04.GC_CC/Whole_new_2020/01.WGS/02.SNV_INDELS/Strelka/SomaticVariants/MERGED/VCF2MAF/Funcotator/funcotator_dataSources.v1.6.20190124s --output 20004_T.somatic.merge.funcotated.maf --output-file-format MAF
21:57:56.549 INFO NativeLibraryLoader - Loading libgkl_compression.so from jar:file:/mnt/ruby/Users/uugi0620/phenomata/Anaconda3/envs/gatk4/share/gatk4-4.1.9.0-0/gatk-package-4.1.9.0-local.jar!/com/intel/gkl/native/libgkl_compression.so
Nov 25, 2020 9:58:03 PM shaded.cloud_nio.com.google.auth.oauth2.ComputeEngineCredentials runningOnComputeEngine
INFO: Failed to detect whether we are running on Google Compute Engine.
21:58:03.732 INFO Funcotator - ------------------------------------------------------------
21:58:03.733 INFO Funcotator - The Genome Analysis Toolkit (GATK) v4.1.9.0
21:58:03.733 INFO Funcotator - For support and documentation go to https://software.broadinstitute.org/gatk/
21:58:03.733 INFO Funcotator - Executing as phenomata@cm156 on Linux v3.10.0-862.14.4.el7.x86_64 amd64
21:58:03.733 INFO Funcotator - Java runtime: OpenJDK 64-Bit Server VM v1.8.0_265-b11
21:58:03.734 INFO Funcotator - Start Date/Time: November 25, 2020 9:57:56 PM KST
21:58:03.734 INFO Funcotator - ------------------------------------------------------------
21:58:03.734 INFO Funcotator - ------------------------------------------------------------
21:58:03.735 INFO Funcotator - HTSJDK Version: 2.23.0
21:58:03.735 INFO Funcotator - Picard Version: 2.23.3
21:58:03.735 INFO Funcotator - HTSJDK Defaults.COMPRESSION_LEVEL : 2
21:58:03.735 INFO Funcotator - HTSJDK Defaults.USE_ASYNC_IO_READ_FOR_SAMTOOLS : false
21:58:03.735 INFO Funcotator - HTSJDK Defaults.USE_ASYNC_IO_WRITE_FOR_SAMTOOLS : true
21:58:03.735 INFO Funcotator - HTSJDK Defaults.USE_ASYNC_IO_WRITE_FOR_TRIBBLE : false
21:58:03.736 INFO Funcotator - Deflater: IntelDeflater
21:58:03.736 INFO Funcotator - Inflater: IntelInflater
21:58:03.736 INFO Funcotator - GCS max retries/reopens: 20
21:58:03.736 INFO Funcotator - Requester pays: disabled
21:58:03.736 INFO Funcotator - Initializing engine
21:58:04.094 INFO FeatureManager - Using codec VCFCodec to read file file:///mnt/clinix1/Analysis/mongol/phenomata/04.GC_CC/Whole_new_2020/01.WGS/02.SNV_INDELS/Strelka/SomaticVariants/MERGED/VCF2MAF/Funcotator/20004_T.somatic.merge.vcf.gz
21:58:04.160 INFO Funcotator - Done initializing engine
21:58:04.160 INFO Funcotator - Validating sequence dictionaries...
21:58:04.161 INFO Funcotator - Processing user transcripts/defaults/overrides...
21:58:04.161 INFO Funcotator - Initializing data sources...
21:58:04.163 INFO DataSourceUtils - Initializing data sources from directory: /clinix1/Analysis/mongol/phenomata/04.GC_CC/Whole_new_2020/01.WGS/02.SNV_INDELS/Strelka/SomaticVariants/MERGED/VCF2MAF/Funcotator/funcotator_dataSources.v1.6.20190124s
21:58:04.165 INFO DataSourceUtils - Data sources version: 1.6.2019124s
21:58:04.165 INFO DataSourceUtils - Data sources source: ftp://gsapubftp-anonymous@ftp.broadinstitute.org/bundle/funcotator/funcotator_dataSources.v1.6.20190124s.tar.gz
21:58:04.165 INFO DataSourceUtils - Data sources alternate source: gs://broad-public-datasets/funcotator/funcotator_dataSources.v1.6.20190124s.tar.gz
21:58:04.206 INFO DataSourceUtils - Resolved data source file path: file:///mnt/clinix1/Analysis/mongol/phenomata/04.GC_CC/Whole_new_2020/01.WGS/02.SNV_INDELS/Strelka/SomaticVariants/MERGED/VCF2MAF/Funcotator/gencode_xhgnc_v75_37.hg19.tsv -> file:///clinix1/Analysis/mongol/phenomata/04.GC_CC/Whole_new_2020/01.WGS/02.SNV_INDELS/Strelka/SomaticVariants/MERGED/VCF2MAF/Funcotator/funcotator_dataSources.v1.6.20190124s/gencode_xhgnc/hg19/gencode_xhgnc_v75_37.hg19.tsv
21:58:04.209 INFO DataSourceUtils - Resolved data source file path: file:///mnt/clinix1/Analysis/mongol/phenomata/04.GC_CC/Whole_new_2020/01.WGS/02.SNV_INDELS/Strelka/SomaticVariants/MERGED/VCF2MAF/Funcotator/Familial_Cancer_Genes.no_dupes.tsv -> file:///clinix1/Analysis/mongol/phenomata/04.GC_CC/Whole_new_2020/01.WGS/02.SNV_INDELS/Strelka/SomaticVariants/MERGED/VCF2MAF/Funcotator/funcotator_dataSources.v1.6.20190124s/familial/hg19/Familial_Cancer_Genes.no_dupes.tsv
21:58:04.212 INFO DataSourceUtils - Resolved data source file path: file:///mnt/clinix1/Analysis/mongol/phenomata/04.GC_CC/Whole_new_2020/01.WGS/02.SNV_INDELS/Strelka/SomaticVariants/MERGED/VCF2MAF/Funcotator/dnaRepairGenes.20180524T145835.csv -> file:///clinix1/Analysis/mongol/phenomata/04.GC_CC/Whole_new_2020/01.WGS/02.SNV_INDELS/Strelka/SomaticVariants/MERGED/VCF2MAF/Funcotator/funcotator_dataSources.v1.6.20190124s/dna_repair_genes/hg19/dnaRepairGenes.20180524T145835.csv
21:58:04.214 INFO DataSourceUtils - Resolved data source file path: file:///mnt/clinix1/Analysis/mongol/phenomata/04.GC_CC/Whole_new_2020/01.WGS/02.SNV_INDELS/Strelka/SomaticVariants/MERGED/VCF2MAF/Funcotator/hg19_All_20170710.vcf.gz -> file:///clinix1/Analysis/mongol/phenomata/04.GC_CC/Whole_new_2020/01.WGS/02.SNV_INDELS/Strelka/SomaticVariants/MERGED/VCF2MAF/Funcotator/funcotator_dataSources.v1.6.20190124s/dbsnp/hg19/hg19_All_20170710.vcf.gz
21:58:04.216 INFO DataSourceUtils - Resolved data source file path: file:///mnt/clinix1/Analysis/mongol/phenomata/04.GC_CC/Whole_new_2020/01.WGS/02.SNV_INDELS/Strelka/SomaticVariants/MERGED/VCF2MAF/Funcotator/Cosmic.db -> file:///clinix1/Analysis/mongol/phenomata/04.GC_CC/Whole_new_2020/01.WGS/02.SNV_INDELS/Strelka/SomaticVariants/MERGED/VCF2MAF/Funcotator/funcotator_dataSources.v1.6.20190124s/cosmic/hg19/Cosmic.db
21:58:04.218 INFO DataSourceUtils - Resolved data source file path: file:///mnt/clinix1/Analysis/mongol/phenomata/04.GC_CC/Whole_new_2020/01.WGS/02.SNV_INDELS/Strelka/SomaticVariants/MERGED/VCF2MAF/Funcotator/clinvar_hgmd.tsv -> file:///clinix1/Analysis/mongol/phenomata/04.GC_CC/Whole_new_2020/01.WGS/02.SNV_INDELS/Strelka/SomaticVariants/MERGED/VCF2MAF/Funcotator/funcotator_dataSources.v1.6.20190124s/clinvar/hg19/clinvar_hgmd.tsv
21:58:04.220 INFO DataSourceUtils - Resolved data source file path: file:///mnt/clinix1/Analysis/mongol/phenomata/04.GC_CC/Whole_new_2020/01.WGS/02.SNV_INDELS/Strelka/SomaticVariants/MERGED/VCF2MAF/Funcotator/simple_uniprot_Dec012014.tsv -> file:///clinix1/Analysis/mongol/phenomata/04.GC_CC/Whole_new_2020/01.WGS/02.SNV_INDELS/Strelka/SomaticVariants/MERGED/VCF2MAF/Funcotator/funcotator_dataSources.v1.6.20190124s/simple_uniprot/hg19/simple_uniprot_Dec012014.tsv
21:58:04.222 INFO DataSourceUtils - Resolved data source file path: file:///mnt/clinix1/Analysis/mongol/phenomata/04.GC_CC/Whole_new_2020/01.WGS/02.SNV_INDELS/Strelka/SomaticVariants/MERGED/VCF2MAF/Funcotator/cosmic_tissue.tsv -> file:///clinix1/Analysis/mongol/phenomata/04.GC_CC/Whole_new_2020/01.WGS/02.SNV_INDELS/Strelka/SomaticVariants/MERGED/VCF2MAF/Funcotator/funcotator_dataSources.v1.6.20190124s/cosmic_tissue/hg19/cosmic_tissue.tsv
21:58:04.225 INFO DataSourceUtils - Resolved data source file path: file:///mnt/clinix1/Analysis/mongol/phenomata/04.GC_CC/Whole_new_2020/01.WGS/02.SNV_INDELS/Strelka/SomaticVariants/MERGED/VCF2MAF/Funcotator/chr1_a_bed.tsv -> file:///clinix1/Analysis/mongol/phenomata/04.GC_CC/Whole_new_2020/01.WGS/02.SNV_INDELS/Strelka/SomaticVariants/MERGED/VCF2MAF/Funcotator/funcotator_dataSources.v1.6.20190124s/chr1_a_bed/hg19/chr1_a_bed.tsv
21:58:04.227 INFO DataSourceUtils - Resolved data source file path: file:///mnt/clinix1/Analysis/mongol/phenomata/04.GC_CC/Whole_new_2020/01.WGS/02.SNV_INDELS/Strelka/SomaticVariants/MERGED/VCF2MAF/Funcotator/achilles_lineage_results.import.txt -> file:///clinix1/Analysis/mongol/phenomata/04.GC_CC/Whole_new_2020/01.WGS/02.SNV_INDELS/Strelka/SomaticVariants/MERGED/VCF2MAF/Funcotator/funcotator_dataSources.v1.6.20190124s/achilles/hg19/achilles_lineage_results.import.txt
21:58:04.229 INFO DataSourceUtils - Resolved data source file path: file:///mnt/clinix1/Analysis/mongol/phenomata/04.GC_CC/Whole_new_2020/01.WGS/02.SNV_INDELS/Strelka/SomaticVariants/MERGED/VCF2MAF/Funcotator/cosmic_fusion.tsv -> file:///clinix1/Analysis/mongol/phenomata/04.GC_CC/Whole_new_2020/01.WGS/02.SNV_INDELS/Strelka/SomaticVariants/MERGED/VCF2MAF/Funcotator/funcotator_dataSources.v1.6.20190124s/cosmic_fusion/hg19/cosmic_fusion.tsv
21:58:04.231 INFO DataSourceUtils - Resolved data source file path: file:///mnt/clinix1/Analysis/mongol/phenomata/04.GC_CC/Whole_new_2020/01.WGS/02.SNV_INDELS/Strelka/SomaticVariants/MERGED/VCF2MAF/Funcotator/hgnc_download_Nov302017.tsv -> file:///clinix1/Analysis/mongol/phenomata/04.GC_CC/Whole_new_2020/01.WGS/02.SNV_INDELS/Strelka/SomaticVariants/MERGED/VCF2MAF/Funcotator/funcotator_dataSources.v1.6.20190124s/hgnc/hg19/hgnc_download_Nov302017.tsv
21:58:04.233 INFO DataSourceUtils - Resolved data source file path: file:///mnt/clinix1/Analysis/mongol/phenomata/04.GC_CC/Whole_new_2020/01.WGS/02.SNV_INDELS/Strelka/SomaticVariants/MERGED/VCF2MAF/Funcotator/gencode_xrefseq_v75_37.tsv -> file:///clinix1/Analysis/mongol/phenomata/04.GC_CC/Whole_new_2020/01.WGS/02.SNV_INDELS/Strelka/SomaticVariants/MERGED/VCF2MAF/Funcotator/funcotator_dataSources.v1.6.20190124s/gencode_xrefseq/hg19/gencode_xrefseq_v75_37.tsv
21:58:04.235 INFO DataSourceUtils - Resolved data source file path: file:///mnt/clinix1/Analysis/mongol/phenomata/04.GC_CC/Whole_new_2020/01.WGS/02.SNV_INDELS/Strelka/SomaticVariants/MERGED/VCF2MAF/Funcotator/CancerGeneCensus_Table_1_full_2012-03-15.txt -> file:///clinix1/Analysis/mongol/phenomata/04.GC_CC/Whole_new_2020/01.WGS/02.SNV_INDELS/Strelka/SomaticVariants/MERGED/VCF2MAF/Funcotator/funcotator_dataSources.v1.6.20190124s/cancer_gene_census/hg19/CancerGeneCensus_Table_1_full_2012-03-15.txt
21:58:04.237 INFO DataSourceUtils - Resolved data source file path: file:///mnt/clinix1/Analysis/mongol/phenomata/04.GC_CC/Whole_new_2020/01.WGS/02.SNV_INDELS/Strelka/SomaticVariants/MERGED/VCF2MAF/Funcotator/oreganno.tsv -> file:///clinix1/Analysis/mongol/phenomata/04.GC_CC/Whole_new_2020/01.WGS/02.SNV_INDELS/Strelka/SomaticVariants/MERGED/VCF2MAF/Funcotator/funcotator_dataSources.v1.6.20190124s/oreganno/hg19/oreganno.tsv
21:58:04.239 INFO DataSourceUtils - Resolved data source file path: file:///mnt/clinix1/Analysis/mongol/phenomata/04.GC_CC/Whole_new_2020/01.WGS/02.SNV_INDELS/Strelka/SomaticVariants/MERGED/VCF2MAF/Funcotator/gencode.v19.annotation.REORDERED.gtf -> file:///clinix1/Analysis/mongol/phenomata/04.GC_CC/Whole_new_2020/01.WGS/02.SNV_INDELS/Strelka/SomaticVariants/MERGED/VCF2MAF/Funcotator/funcotator_dataSources.v1.6.20190124s/gencode/hg19/gencode.v19.annotation.REORDERED.gtf
21:58:04.240 INFO DataSourceUtils - Resolved data source file path: file:///mnt/clinix1/Analysis/mongol/phenomata/04.GC_CC/Whole_new_2020/01.WGS/02.SNV_INDELS/Strelka/SomaticVariants/MERGED/VCF2MAF/Funcotator/gencode.v19.pc_transcripts.fa -> file:///clinix1/Analysis/mongol/phenomata/04.GC_CC/Whole_new_2020/01.WGS/02.SNV_INDELS/Strelka/SomaticVariants/MERGED/VCF2MAF/Funcotator/funcotator_dataSources.v1.6.20190124s/gencode/hg19/gencode.v19.pc_transcripts.fa
21:58:04.242 INFO DataSourceUtils - Resolved data source file path: file:///mnt/clinix1/Analysis/mongol/phenomata/04.GC_CC/Whole_new_2020/01.WGS/02.SNV_INDELS/Strelka/SomaticVariants/MERGED/VCF2MAF/Funcotator/chr1_b_bed.tsv -> file:///clinix1/Analysis/mongol/phenomata/04.GC_CC/Whole_new_2020/01.WGS/02.SNV_INDELS/Strelka/SomaticVariants/MERGED/VCF2MAF/Funcotator/funcotator_dataSources.v1.6.20190124s/chr1_b_bed/hg19/chr1_b_bed.tsv
21:58:04.242 INFO Funcotator - Finalizing data sources (this step can be long if data sources are cloud-based)...
21:58:04.243 INFO DataSourceUtils - Resolved data source file path: file:///mnt/clinix1/Analysis/mongol/phenomata/04.GC_CC/Whole_new_2020/01.WGS/02.SNV_INDELS/Strelka/SomaticVariants/MERGED/VCF2MAF/Funcotator/gencode_xhgnc_v75_37.hg19.tsv -> file:///clinix1/Analysis/mongol/phenomata/04.GC_CC/Whole_new_2020/01.WGS/02.SNV_INDELS/Strelka/SomaticVariants/MERGED/VCF2MAF/Funcotator/funcotator_dataSources.v1.6.20190124s/gencode_xhgnc/hg19/gencode_xhgnc_v75_37.hg19.tsv
21:58:04.969 INFO DataSourceUtils - Resolved data source file path: file:///mnt/clinix1/Analysis/mongol/phenomata/04.GC_CC/Whole_new_2020/01.WGS/02.SNV_INDELS/Strelka/SomaticVariants/MERGED/VCF2MAF/Funcotator/Familial_Cancer_Genes.no_dupes.tsv -> file:///clinix1/Analysis/mongol/phenomata/04.GC_CC/Whole_new_2020/01.WGS/02.SNV_INDELS/Strelka/SomaticVariants/MERGED/VCF2MAF/Funcotator/funcotator_dataSources.v1.6.20190124s/familial/hg19/Familial_Cancer_Genes.no_dupes.tsv
21:58:04.971 INFO DataSourceUtils - Resolved data source file path: file:///mnt/clinix1/Analysis/mongol/phenomata/04.GC_CC/Whole_new_2020/01.WGS/02.SNV_INDELS/Strelka/SomaticVariants/MERGED/VCF2MAF/Funcotator/dnaRepairGenes.20180524T145835.csv -> file:///clinix1/Analysis/mongol/phenomata/04.GC_CC/Whole_new_2020/01.WGS/02.SNV_INDELS/Strelka/SomaticVariants/MERGED/VCF2MAF/Funcotator/funcotator_dataSources.v1.6.20190124s/dna_repair_genes/hg19/dnaRepairGenes.20180524T145835.csv
21:58:04.972 INFO DataSourceUtils - Resolved data source file path: file:///mnt/clinix1/Analysis/mongol/phenomata/04.GC_CC/Whole_new_2020/01.WGS/02.SNV_INDELS/Strelka/SomaticVariants/MERGED/VCF2MAF/Funcotator/hg19_All_20170710.vcf.gz -> file:///clinix1/Analysis/mongol/phenomata/04.GC_CC/Whole_new_2020/01.WGS/02.SNV_INDELS/Strelka/SomaticVariants/MERGED/VCF2MAF/Funcotator/funcotator_dataSources.v1.6.20190124s/dbsnp/hg19/hg19_All_20170710.vcf.gz
21:58:04.972 INFO DataSourceUtils - Setting lookahead cache for data source: dbSNP : 100000
21:58:04.993 INFO FeatureManager - Using codec VCFCodec to read file file:///clinix1/Analysis/mongol/phenomata/04.GC_CC/Whole_new_2020/01.WGS/02.SNV_INDELS/Strelka/SomaticVariants/MERGED/VCF2MAF/Funcotator/funcotator_dataSources.v1.6.20190124s/dbsnp/hg19/hg19_All_20170710.vcf.gz
21:58:05.071 INFO DataSourceUtils - Resolved data source file path: file:///mnt/clinix1/Analysis/mongol/phenomata/04.GC_CC/Whole_new_2020/01.WGS/02.SNV_INDELS/Strelka/SomaticVariants/MERGED/VCF2MAF/Funcotator/hg19_All_20170710.vcf.gz -> file:///clinix1/Analysis/mongol/phenomata/04.GC_CC/Whole_new_2020/01.WGS/02.SNV_INDELS/Strelka/SomaticVariants/MERGED/VCF2MAF/Funcotator/funcotator_dataSources.v1.6.20190124s/dbsnp/hg19/hg19_All_20170710.vcf.gz
21:58:05.119 INFO FeatureManager - Using codec VCFCodec to read file file:///clinix1/Analysis/mongol/phenomata/04.GC_CC/Whole_new_2020/01.WGS/02.SNV_INDELS/Strelka/SomaticVariants/MERGED/VCF2MAF/Funcotator/funcotator_dataSources.v1.6.20190124s/dbsnp/hg19/hg19_All_20170710.vcf.gz
21:58:05.179 INFO DataSourceUtils - Resolved data source file path: file:///mnt/clinix1/Analysis/mongol/phenomata/04.GC_CC/Whole_new_2020/01.WGS/02.SNV_INDELS/Strelka/SomaticVariants/MERGED/VCF2MAF/Funcotator/Cosmic.db -> file:///clinix1/Analysis/mongol/phenomata/04.GC_CC/Whole_new_2020/01.WGS/02.SNV_INDELS/Strelka/SomaticVariants/MERGED/VCF2MAF/Funcotator/funcotator_dataSources.v1.6.20190124s/cosmic/hg19/Cosmic.db
21:58:05.325 INFO DataSourceUtils - Setting lookahead cache for data source: ClinVar : 100000
21:58:05.331 INFO DataSourceUtils - Resolved data source file path: file:///mnt/clinix1/Analysis/mongol/phenomata/04.GC_CC/Whole_new_2020/01.WGS/02.SNV_INDELS/Strelka/SomaticVariants/MERGED/VCF2MAF/Funcotator/clinvar_hgmd.tsv -> file:///clinix1/Analysis/mongol/phenomata/04.GC_CC/Whole_new_2020/01.WGS/02.SNV_INDELS/Strelka/SomaticVariants/MERGED/VCF2MAF/Funcotator/funcotator_dataSources.v1.6.20190124s/clinvar/hg19/clinvar_hgmd.tsv
21:58:05.332 INFO FeatureManager - Using codec XsvLocatableTableCodec to read file file:///clinix1/Analysis/mongol/phenomata/04.GC_CC/Whole_new_2020/01.WGS/02.SNV_INDELS/Strelka/SomaticVariants/MERGED/VCF2MAF/Funcotator/funcotator_dataSources.v1.6.20190124s/clinvar/hg19/clinvar_hgmd.config
21:58:05.423 INFO DataSourceUtils - Resolved data source file path: file:///mnt/clinix1/Analysis/mongol/phenomata/04.GC_CC/Whole_new_2020/01.WGS/02.SNV_INDELS/Strelka/SomaticVariants/MERGED/VCF2MAF/Funcotator/clinvar_hgmd.tsv -> file:///clinix1/Analysis/mongol/phenomata/04.GC_CC/Whole_new_2020/01.WGS/02.SNV_INDELS/Strelka/SomaticVariants/MERGED/VCF2MAF/Funcotator/funcotator_dataSources.v1.6.20190124s/clinvar/hg19/clinvar_hgmd.tsv
21:58:05.423 INFO DataSourceUtils - Resolved data source file path: file:///mnt/clinix1/Analysis/mongol/phenomata/04.GC_CC/Whole_new_2020/01.WGS/02.SNV_INDELS/Strelka/SomaticVariants/MERGED/VCF2MAF/Funcotator/clinvar_hgmd.tsv -> file:///clinix1/Analysis/mongol/phenomata/04.GC_CC/Whole_new_2020/01.WGS/02.SNV_INDELS/Strelka/SomaticVariants/MERGED/VCF2MAF/Funcotator/funcotator_dataSources.v1.6.20190124s/clinvar/hg19/clinvar_hgmd.tsv
WARNING 2020-11-25 21:58:05 AsciiLineReader Creating an indexable source for an AsciiFeatureCodec using a stream that is neither a PositionalBufferedStream nor a BlockCompressedInputStream
21:58:05.428 INFO DataSourceUtils - Resolved data source file path: file:///mnt/clinix1/Analysis/mongol/phenomata/04.GC_CC/Whole_new_2020/01.WGS/02.SNV_INDELS/Strelka/SomaticVariants/MERGED/VCF2MAF/Funcotator/simple_uniprot_Dec012014.tsv -> file:///clinix1/Analysis/mongol/phenomata/04.GC_CC/Whole_new_2020/01.WGS/02.SNV_INDELS/Strelka/SomaticVariants/MERGED/VCF2MAF/Funcotator/funcotator_dataSources.v1.6.20190124s/simple_uniprot/hg19/simple_uniprot_Dec012014.tsv
21:58:05.474 INFO DataSourceUtils - Resolved data source file path: file:///mnt/clinix1/Analysis/mongol/phenomata/04.GC_CC/Whole_new_2020/01.WGS/02.SNV_INDELS/Strelka/SomaticVariants/MERGED/VCF2MAF/Funcotator/cosmic_tissue.tsv -> file:///clinix1/Analysis/mongol/phenomata/04.GC_CC/Whole_new_2020/01.WGS/02.SNV_INDELS/Strelka/SomaticVariants/MERGED/VCF2MAF/Funcotator/funcotator_dataSources.v1.6.20190124s/cosmic_tissue/hg19/cosmic_tissue.tsv
21:58:05.490 INFO DataSourceUtils - Setting lookahead cache for data source: chr1_a_bed : 100000
21:58:05.494 INFO DataSourceUtils - Resolved data source file path: file:///mnt/clinix1/Analysis/mongol/phenomata/04.GC_CC/Whole_new_2020/01.WGS/02.SNV_INDELS/Strelka/SomaticVariants/MERGED/VCF2MAF/Funcotator/chr1_a_bed.tsv -> file:///clinix1/Analysis/mongol/phenomata/04.GC_CC/Whole_new_2020/01.WGS/02.SNV_INDELS/Strelka/SomaticVariants/MERGED/VCF2MAF/Funcotator/funcotator_dataSources.v1.6.20190124s/chr1_a_bed/hg19/chr1_a_bed.tsv
21:58:05.495 INFO FeatureManager - Using codec XsvLocatableTableCodec to read file file:///clinix1/Analysis/mongol/phenomata/04.GC_CC/Whole_new_2020/01.WGS/02.SNV_INDELS/Strelka/SomaticVariants/MERGED/VCF2MAF/Funcotator/funcotator_dataSources.v1.6.20190124s/chr1_a_bed/hg19/chr1_a_bed.config
21:58:05.498 INFO DataSourceUtils - Resolved data source file path: file:///mnt/clinix1/Analysis/mongol/phenomata/04.GC_CC/Whole_new_2020/01.WGS/02.SNV_INDELS/Strelka/SomaticVariants/MERGED/VCF2MAF/Funcotator/chr1_a_bed.tsv -> file:///clinix1/Analysis/mongol/phenomata/04.GC_CC/Whole_new_2020/01.WGS/02.SNV_INDELS/Strelka/SomaticVariants/MERGED/VCF2MAF/Funcotator/funcotator_dataSources.v1.6.20190124s/chr1_a_bed/hg19/chr1_a_bed.tsv
21:58:05.498 INFO DataSourceUtils - Resolved data source file path: file:///mnt/clinix1/Analysis/mongol/phenomata/04.GC_CC/Whole_new_2020/01.WGS/02.SNV_INDELS/Strelka/SomaticVariants/MERGED/VCF2MAF/Funcotator/chr1_a_bed.tsv -> file:///clinix1/Analysis/mongol/phenomata/04.GC_CC/Whole_new_2020/01.WGS/02.SNV_INDELS/Strelka/SomaticVariants/MERGED/VCF2MAF/Funcotator/funcotator_dataSources.v1.6.20190124s/chr1_a_bed/hg19/chr1_a_bed.tsv
WARNING 2020-11-25 21:58:05 AsciiLineReader Creating an indexable source for an AsciiFeatureCodec using a stream that is neither a PositionalBufferedStream nor a BlockCompressedInputStream
21:58:05.500 INFO DataSourceUtils - Resolved data source file path: file:///mnt/clinix1/Analysis/mongol/phenomata/04.GC_CC/Whole_new_2020/01.WGS/02.SNV_INDELS/Strelka/SomaticVariants/MERGED/VCF2MAF/Funcotator/achilles_lineage_results.import.txt -> file:///clinix1/Analysis/mongol/phenomata/04.GC_CC/Whole_new_2020/01.WGS/02.SNV_INDELS/Strelka/SomaticVariants/MERGED/VCF2MAF/Funcotator/funcotator_dataSources.v1.6.20190124s/achilles/hg19/achilles_lineage_results.import.txt
21:58:05.503 INFO DataSourceUtils - Resolved data source file path: file:///mnt/clinix1/Analysis/mongol/phenomata/04.GC_CC/Whole_new_2020/01.WGS/02.SNV_INDELS/Strelka/SomaticVariants/MERGED/VCF2MAF/Funcotator/cosmic_fusion.tsv -> file:///clinix1/Analysis/mongol/phenomata/04.GC_CC/Whole_new_2020/01.WGS/02.SNV_INDELS/Strelka/SomaticVariants/MERGED/VCF2MAF/Funcotator/funcotator_dataSources.v1.6.20190124s/cosmic_fusion/hg19/cosmic_fusion.tsv
21:58:05.508 INFO DataSourceUtils - Resolved data source file path: file:///mnt/clinix1/Analysis/mongol/phenomata/04.GC_CC/Whole_new_2020/01.WGS/02.SNV_INDELS/Strelka/SomaticVariants/MERGED/VCF2MAF/Funcotator/hgnc_download_Nov302017.tsv -> file:///clinix1/Analysis/mongol/phenomata/04.GC_CC/Whole_new_2020/01.WGS/02.SNV_INDELS/Strelka/SomaticVariants/MERGED/VCF2MAF/Funcotator/funcotator_dataSources.v1.6.20190124s/hgnc/hg19/hgnc_download_Nov302017.tsv
21:58:05.720 INFO DataSourceUtils - Resolved data source file path: file:///mnt/clinix1/Analysis/mongol/phenomata/04.GC_CC/Whole_new_2020/01.WGS/02.SNV_INDELS/Strelka/SomaticVariants/MERGED/VCF2MAF/Funcotator/gencode_xrefseq_v75_37.tsv -> file:///clinix1/Analysis/mongol/phenomata/04.GC_CC/Whole_new_2020/01.WGS/02.SNV_INDELS/Strelka/SomaticVariants/MERGED/VCF2MAF/Funcotator/funcotator_dataSources.v1.6.20190124s/gencode_xrefseq/hg19/gencode_xrefseq_v75_37.tsv
21:58:05.771 INFO DataSourceUtils - Resolved data source file path: file:///mnt/clinix1/Analysis/mongol/phenomata/04.GC_CC/Whole_new_2020/01.WGS/02.SNV_INDELS/Strelka/SomaticVariants/MERGED/VCF2MAF/Funcotator/CancerGeneCensus_Table_1_full_2012-03-15.txt -> file:///clinix1/Analysis/mongol/phenomata/04.GC_CC/Whole_new_2020/01.WGS/02.SNV_INDELS/Strelka/SomaticVariants/MERGED/VCF2MAF/Funcotator/funcotator_dataSources.v1.6.20190124s/cancer_gene_census/hg19/CancerGeneCensus_Table_1_full_2012-03-15.txt
21:58:05.772 INFO DataSourceUtils - Setting lookahead cache for data source: Oreganno : 100000
21:58:05.776 INFO DataSourceUtils - Resolved data source file path: file:///mnt/clinix1/Analysis/mongol/phenomata/04.GC_CC/Whole_new_2020/01.WGS/02.SNV_INDELS/Strelka/SomaticVariants/MERGED/VCF2MAF/Funcotator/oreganno.tsv -> file:///clinix1/Analysis/mongol/phenomata/04.GC_CC/Whole_new_2020/01.WGS/02.SNV_INDELS/Strelka/SomaticVariants/MERGED/VCF2MAF/Funcotator/funcotator_dataSources.v1.6.20190124s/oreganno/hg19/oreganno.tsv
21:58:05.777 INFO FeatureManager - Using codec XsvLocatableTableCodec to read file file:///clinix1/Analysis/mongol/phenomata/04.GC_CC/Whole_new_2020/01.WGS/02.SNV_INDELS/Strelka/SomaticVariants/MERGED/VCF2MAF/Funcotator/funcotator_dataSources.v1.6.20190124s/oreganno/hg19/oreganno.config
21:58:05.795 INFO DataSourceUtils - Resolved data source file path: file:///mnt/clinix1/Analysis/mongol/phenomata/04.GC_CC/Whole_new_2020/01.WGS/02.SNV_INDELS/Strelka/SomaticVariants/MERGED/VCF2MAF/Funcotator/oreganno.tsv -> file:///clinix1/Analysis/mongol/phenomata/04.GC_CC/Whole_new_2020/01.WGS/02.SNV_INDELS/Strelka/SomaticVariants/MERGED/VCF2MAF/Funcotator/funcotator_dataSources.v1.6.20190124s/oreganno/hg19/oreganno.tsv
21:58:05.796 INFO DataSourceUtils - Resolved data source file path: file:///mnt/clinix1/Analysis/mongol/phenomata/04.GC_CC/Whole_new_2020/01.WGS/02.SNV_INDELS/Strelka/SomaticVariants/MERGED/VCF2MAF/Funcotator/oreganno.tsv -> file:///clinix1/Analysis/mongol/phenomata/04.GC_CC/Whole_new_2020/01.WGS/02.SNV_INDELS/Strelka/SomaticVariants/MERGED/VCF2MAF/Funcotator/funcotator_dataSources.v1.6.20190124s/oreganno/hg19/oreganno.tsv
WARNING 2020-11-25 21:58:05 AsciiLineReader Creating an indexable source for an AsciiFeatureCodec using a stream that is neither a PositionalBufferedStream nor a BlockCompressedInputStream
21:58:05.798 INFO DataSourceUtils - Resolved data source file path: file:///mnt/clinix1/Analysis/mongol/phenomata/04.GC_CC/Whole_new_2020/01.WGS/02.SNV_INDELS/Strelka/SomaticVariants/MERGED/VCF2MAF/Funcotator/gencode.v19.annotation.REORDERED.gtf -> file:///clinix1/Analysis/mongol/phenomata/04.GC_CC/Whole_new_2020/01.WGS/02.SNV_INDELS/Strelka/SomaticVariants/MERGED/VCF2MAF/Funcotator/funcotator_dataSources.v1.6.20190124s/gencode/hg19/gencode.v19.annotation.REORDERED.gtf
21:58:05.798 INFO DataSourceUtils - Setting lookahead cache for data source: Gencode : 100000
21:58:05.801 INFO FeatureManager - Using codec GencodeGtfCodec to read file file:///clinix1/Analysis/mongol/phenomata/04.GC_CC/Whole_new_2020/01.WGS/02.SNV_INDELS/Strelka/SomaticVariants/MERGED/VCF2MAF/Funcotator/funcotator_dataSources.v1.6.20190124s/gencode/hg19/gencode.v19.annotation.REORDERED.gtf
21:58:05.808 INFO DataSourceUtils - Resolved data source file path: file:///mnt/clinix1/Analysis/mongol/phenomata/04.GC_CC/Whole_new_2020/01.WGS/02.SNV_INDELS/Strelka/SomaticVariants/MERGED/VCF2MAF/Funcotator/gencode.v19.pc_transcripts.fa -> file:///clinix1/Analysis/mongol/phenomata/04.GC_CC/Whole_new_2020/01.WGS/02.SNV_INDELS/Strelka/SomaticVariants/MERGED/VCF2MAF/Funcotator/funcotator_dataSources.v1.6.20190124s/gencode/hg19/gencode.v19.pc_transcripts.fa
21:58:10.064 INFO DataSourceUtils - Setting lookahead cache for data source: chr1_b_bed : 100000
21:58:10.068 INFO DataSourceUtils - Resolved data source file path: file:///mnt/clinix1/Analysis/mongol/phenomata/04.GC_CC/Whole_new_2020/01.WGS/02.SNV_INDELS/Strelka/SomaticVariants/MERGED/VCF2MAF/Funcotator/chr1_b_bed.tsv -> file:///clinix1/Analysis/mongol/phenomata/04.GC_CC/Whole_new_2020/01.WGS/02.SNV_INDELS/Strelka/SomaticVariants/MERGED/VCF2MAF/Funcotator/funcotator_dataSources.v1.6.20190124s/chr1_b_bed/hg19/chr1_b_bed.tsv
21:58:10.069 INFO FeatureManager - Using codec XsvLocatableTableCodec to read file file:///clinix1/Analysis/mongol/phenomata/04.GC_CC/Whole_new_2020/01.WGS/02.SNV_INDELS/Strelka/SomaticVariants/MERGED/VCF2MAF/Funcotator/funcotator_dataSources.v1.6.20190124s/chr1_b_bed/hg19/chr1_b_bed.config
21:58:10.071 INFO DataSourceUtils - Resolved data source file path: file:///mnt/clinix1/Analysis/mongol/phenomata/04.GC_CC/Whole_new_2020/01.WGS/02.SNV_INDELS/Strelka/SomaticVariants/MERGED/VCF2MAF/Funcotator/chr1_b_bed.tsv -> file:///clinix1/Analysis/mongol/phenomata/04.GC_CC/Whole_new_2020/01.WGS/02.SNV_INDELS/Strelka/SomaticVariants/MERGED/VCF2MAF/Funcotator/funcotator_dataSources.v1.6.20190124s/chr1_b_bed/hg19/chr1_b_bed.tsv
21:58:10.072 INFO DataSourceUtils - Resolved data source file path: file:///mnt/clinix1/Analysis/mongol/phenomata/04.GC_CC/Whole_new_2020/01.WGS/02.SNV_INDELS/Strelka/SomaticVariants/MERGED/VCF2MAF/Funcotator/chr1_b_bed.tsv -> file:///clinix1/Analysis/mongol/phenomata/04.GC_CC/Whole_new_2020/01.WGS/02.SNV_INDELS/Strelka/SomaticVariants/MERGED/VCF2MAF/Funcotator/funcotator_dataSources.v1.6.20190124s/chr1_b_bed/hg19/chr1_b_bed.tsv
WARNING 2020-11-25 21:58:10 AsciiLineReader Creating an indexable source for an AsciiFeatureCodec using a stream that is neither a PositionalBufferedStream nor a BlockCompressedInputStream
21:58:10.073 INFO Funcotator - Initializing Funcotator Engine...
21:58:10.094 INFO FuncotatorEngine - VCF sequence dictionary detected as B37 in HG19 annotation mode. Performing conversion.
21:58:10.094 WARN FuncotatorEngine - WARNING: You are using B37 as a reference. Funcotator will convert your variants to GRCh37, and this will be fine in the vast majority of cases. There MAY be some errors (e.g. in the Y chromosome, but possibly in other places as well) due to changes between the two references.
21:58:10.094 INFO Funcotator - Creating a MAF file for output: file:/mnt/clinix1/Analysis/mongol/phenomata/04.GC_CC/Whole_new_2020/01.WGS/02.SNV_INDELS/Strelka/SomaticVariants/MERGED/VCF2MAF/Funcotator/20004_T.somatic.merge.funcotated.maf
21:58:10.105 WARN MafOutputRenderer - No tumor/normal pairs were seen, cannot populate the some of the MAF fields (e.g. t_alt_count). Please add '##tumor_sample=<tumor_sample_name>' and (if applicable) '##normal_sample=<normal_sample_name>' to the input VCF header
21:58:10.149 INFO ProgressMeter - Starting traversal
21:58:10.149 INFO ProgressMeter - Current Locus Elapsed Minutes Variants Processed Variants/Minute
21:58:10.337 INFO VcfFuncotationFactory - dbSNP 9606_b150 cache hits/total: 0/0
21:58:10.346 INFO Funcotator - Shutting down engine
[November 25, 2020 9:58:10 PM KST] org.broadinstitute.hellbender.tools.funcotator.Funcotator done. Elapsed time: 0.23 minutes.
Runtime.totalMemory()=3271557120
org.broadinstitute.hellbender.exceptions.GATKException: Unable to query the database for geneName: RP11-206L10.8
at org.broadinstitute.hellbender.tools.funcotator.dataSources.cosmic.CosmicFuncotationFactory.createFuncotationsOnVariant(CosmicFuncotationFactory.java:320)
at org.broadinstitute.hellbender.tools.funcotator.DataSourceFuncotationFactory.determineFuncotations(DataSourceFuncotationFactory.java:245)
at org.broadinstitute.hellbender.tools.funcotator.DataSourceFuncotationFactory.createFuncotations(DataSourceFuncotationFactory.java:211)
at org.broadinstitute.hellbender.tools.funcotator.FuncotatorEngine.createFuncotationMapForVariant(FuncotatorEngine.java:173)
at org.broadinstitute.hellbender.tools.funcotator.Funcotator.enqueueAndHandleVariant(Funcotator.java:907)
at org.broadinstitute.hellbender.tools.funcotator.Funcotator.apply(Funcotator.java:861)
at org.broadinstitute.hellbender.engine.VariantWalker.lambda$traverse$0(VariantWalker.java:104)
at java.util.stream.ForEachOps$ForEachOp$OfRef.accept(ForEachOps.java:183)
at java.util.stream.ReferencePipeline$3$1.accept(ReferencePipeline.java:193)
at java.util.stream.ReferencePipeline$2$1.accept(ReferencePipeline.java:175)
at java.util.stream.ReferencePipeline$3$1.accept(ReferencePipeline.java:193)
at java.util.Iterator.forEachRemaining(Iterator.java:116)
at java.util.Spliterators$IteratorSpliterator.forEachRemaining(Spliterators.java:1801)
at java.util.stream.AbstractPipeline.copyInto(AbstractPipeline.java:482)
at java.util.stream.AbstractPipeline.wrapAndCopyInto(AbstractPipeline.java:472)
at java.util.stream.ForEachOps$ForEachOp.evaluateSequential(ForEachOps.java:150)
at java.util.stream.ForEachOps$ForEachOp$OfRef.evaluateSequential(ForEachOps.java:173)
at java.util.stream.AbstractPipeline.evaluate(AbstractPipeline.java:234)
at java.util.stream.ReferencePipeline.forEach(ReferencePipeline.java:485)
at org.broadinstitute.hellbender.engine.VariantWalker.traverse(VariantWalker.java:102)
at org.broadinstitute.hellbender.engine.GATKTool.doWork(GATKTool.java:1049)
at org.broadinstitute.hellbender.cmdline.CommandLineProgram.runTool(CommandLineProgram.java:140)
at org.broadinstitute.hellbender.cmdline.CommandLineProgram.instanceMainPostParseArgs(CommandLineProgram.java:192)
at org.broadinstitute.hellbender.cmdline.CommandLineProgram.instanceMain(CommandLineProgram.java:211)
at org.broadinstitute.hellbender.Main.runCommandLineProgram(Main.java:160)
at org.broadinstitute.hellbender.Main.mainEntry(Main.java:203)
at org.broadinstitute.hellbender.Main.main(Main.java:289)
Caused by: org.sqlite.SQLiteException: [SQLITE_IOERR_LOCK] I/O error in the advisory file locking logic (disk I/O error)
at org.sqlite.core.DB.newSQLException(DB.java:909)
at org.sqlite.core.DB.newSQLException(DB.java:921)
at org.sqlite.core.DB.throwex(DB.java:886)
at org.sqlite.core.NativeDB.prepare_utf8(Native Method)
at org.sqlite.core.NativeDB.prepare(NativeDB.java:127)
at org.sqlite.core.DB.prepare(DB.java:227)
at org.sqlite.jdbc3.JDBC3Statement.executeQuery(JDBC3Statement.java:81)
at org.broadinstitute.hellbender.tools.funcotator.dataSources.cosmic.CosmicFuncotationFactory.createFuncotationsOnVariant(CosmicFuncotationFactory.java:288)
... 26 more
Note:
- Reference fasta i'm using is b37 reference with hs37d5 decoy sequence.
- Somatic variant calling was done by Strelka2
- Using funcotator_dataSources.v1.7.20200521s as a data source, similar error has occurred except for the gene funcotator having trouble with:
org.broadinstitute.hellbender.exceptions.GATKException: Unable to query the database for geneName: LINC01409
Oddly, I've checked whether RP11-206L10.8 is in the gencode hg19 database and it turned out this gene is in the gencode.v19.annotation.REORDERED.gtf file but not in gencode.v19.pc_transcripts.fa
grep 'RP11-206L10.8' gencode.v19.pc_transcripts.fa # No output
grep 'RP11-206L10.8' gencode.v19.annotation.REORDERED.gtf
chr1 HAVANA gene 736259 745541 . - . gene_id "ENSG00000230092.3"; transcript_id "ENSG00000230092.3"; gene_type "pseudogene"; gene_status "KNOWN"; gene_name "RP11-206L10.8"; transcript_type "pseudogene"; transcript_status "KNOWN"; transcript_name "RP11-206L10.8"; level 2; havana_gene "OTTHUMG00000002403.2";
chr1 HAVANA transcript 736259 745541 . - . gene_id "ENSG00000230092.3"; transcript_id "ENST00000590817.1"; gene_type "pseudogene"; gene_status "KNOWN"; gene_name "RP11-206L10.8"; transcript_type "transcribed_unprocessed_pseudogene"; transcript_status "KNOWN"; transcript_name "RP11-206L10.8-001"; level 2; ont "PGO:0000005"; ont "PGO:0000019"; havana_gene "OTTHUMG00000002403.2"; havana_transcript "OTTHUMT00000006886.1";
chr1 HAVANA exon 745447 745541 . - . gene_id "ENSG00000230092.3"; transcript_id "ENST00000590817.1"; gene_type "pseudogene"; gene_status "KNOWN"; gene_name "RP11-206L10.8"; transcript_type "transcribed_unprocessed_pseudogene"; transcript_status "KNOWN"; transcript_name "RP11-206L10.8-001"; exon_number 1; exon_id "ENSE00001651951.1"; level 2; ont "PGO:0000005"; ont "PGO:0000019"; havana_gene "OTTHUMG00000002403.2"; havana_transcript "OTTHUMT00000006886.1";
chr1 HAVANA exon 743954 744003 . - . gene_id "ENSG00000230092.3"; transcript_id "ENST00000590817.1"; gene_type "pseudogene"; gene_status "KNOWN"; gene_name "RP11-206L10.8"; transcript_type "transcribed_unprocessed_pseudogene"; transcript_status "KNOWN"; transcript_name "RP11-206L10.8-001"; exon_number 2; exon_id "ENSE00001782999.1"; level 2; ont "PGO:0000005"; ont "PGO:0000019"; havana_gene "OTTHUMG00000002403.2"; havana_transcript "OTTHUMT00000006886.1";
chr1 HAVANA exon 741179 741271 . - . gene_id "ENSG00000230092.3"; transcript_id "ENST00000590817.1"; gene_type "pseudogene"; gene_status "KNOWN"; gene_name "RP11-206L10.8"; transcript_type "transcribed_unprocessed_pseudogene"; transcript_status "KNOWN"; transcript_name "RP11-206L10.8-001"; exon_number 3; exon_id "ENSE00001640641.1"; level 2; ont "PGO:0000005"; ont "PGO:0000019"; havana_gene "OTTHUMG00000002403.2"; havana_transcript "OTTHUMT00000006886.1";
chr1 HAVANA exon 736259 736543 . - . gene_id "ENSG00000230092.3"; transcript_id "ENST00000590817.1"; gene_type "pseudogene"; gene_status "KNOWN"; gene_name "RP11-206L10.8"; transcript_type "transcribed_unprocessed_pseudogene"; transcript_status "KNOWN"; transcript_name "RP11-206L10.8-001"; exon_number 4; exon_id "ENSE00001716345.1"; level 2; ont "PGO:0000005"; ont "PGO:0000019"; havana_gene "OTTHUMG00000002403.2"; havana_transcript "OTTHUMT00000006886.1";
chr1 HAVANA transcript 736259 745541 . - . gene_id "ENSG00000230092.3"; transcript_id "ENST00000447500.1"; gene_type "pseudogene"; gene_status "KNOWN"; gene_name "RP11-206L10.8"; transcript_type "processed_transcript"; transcript_status "KNOWN"; transcript_name "RP11-206L10.8-002"; level 2; tag "basic"; havana_gene "OTTHUMG00000002403.2"; havana_transcript "OTTHUMT00000448550.1";
chr1 HAVANA exon 745447 745541 . - . gene_id "ENSG00000230092.3"; transcript_id "ENST00000447500.1"; gene_type "pseudogene"; gene_status "KNOWN"; gene_name "RP11-206L10.8"; transcript_type "processed_transcript"; transcript_status "KNOWN"; transcript_name "RP11-206L10.8-002"; exon_number 1; exon_id "ENSE00001651951.1"; level 2; tag "basic"; havana_gene "OTTHUMG00000002403.2"; havana_transcript "OTTHUMT00000448550.1";
chr1 HAVANA exon 743954 744003 . - . gene_id "ENSG00000230092.3"; transcript_id "ENST00000447500.1"; gene_type "pseudogene"; gene_status "KNOWN"; gene_name "RP11-206L10.8"; transcript_type "processed_transcript"; transcript_status "KNOWN"; transcript_name "RP11-206L10.8-002"; exon_number 2; exon_id "ENSE00001782999.1"; level 2; tag "basic"; havana_gene "OTTHUMG00000002403.2"; havana_transcript "OTTHUMT00000448550.1";
chr1 HAVANA exon 741179 741271 . - . gene_id "ENSG00000230092.3"; transcript_id "ENST00000447500.1"; gene_type "pseudogene"; gene_status "KNOWN"; gene_name "RP11-206L10.8"; transcript_type "processed_transcript"; transcript_status "KNOWN"; transcript_name "RP11-206L10.8-002"; exon_number 3; exon_id "ENSE00001640641.1"; level 2; tag "basic"; havana_gene "OTTHUMG00000002403.2"; havana_transcript "OTTHUMT00000448550.1";
chr1 HAVANA exon 736259 736543 . - . gene_id "ENSG00000230092.3"; transcript_id "ENST00000447500.1"; gene_type "pseudogene"; gene_status "KNOWN"; gene_name "RP11-206L10.8"; transcript_type "processed_transcript"; transcript_status "KNOWN"; transcript_name "RP11-206L10.8-002"; exon_number 4; exon_id "ENSE00001716345.1"; level 2; tag "basic"; havana_gene "OTTHUMG00000002403.2"; havana_transcript "OTTHUMT00000448550.1";
Is there anything I can do to fix this issue?
Best regards,
Ryan
-
Hi Min-Hwan Sohn,
This does not look like an issue with your files or reference file versions. We have seen this issue before and there is more information at that link about what might be going on. It is most likely an issue with your file system and SQL Lite not working well with that system.
What is your file system where you are running and storing Funcotator?
You may be able to disable the cosmic data source which will remove the SQL Lite requirements. You can also try to run it on your local or non-distributed file system.
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