Genome Analysis Toolkit

Variant Discovery in High-Throughput Sequencing Data

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Developed in the Data Sciences Platform at the Broad Institute, the toolkit offers a wide variety of tools with a primary focus on variant discovery and genotyping. Its powerful processing engine and high-performance computing features make it capable of taking on projects of any size. Learn more

Funcotator - WARN GencodeFuncotationFactory - Cannot create complete funcotation for variant at chr1:984456-984456 due to alternate allele: *

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    Genevieve Brandt (she/her)

    Hi Manuela Bonetto, this is a known issue with Funcotator. (See this link for more details). The best workaround would be to remove the problematic variant from your file. You can inspect the output file from Funcotator and find the last variant in the file. Find that same variant in the input file, the following variant is the variant causing this issue.

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