Dear GATK Team,
The (How to) Map and clean up short read sequence data efficiently tutorial describes:
For multiplexed samples, first perform the workflow steps on a file representing one sample and one lane. Then mark duplicates. Later, after some steps in the GATK's variant discovery workflow, and after aggregating files from the same sample from across lanes into a single file, mark duplicates again. These two marking steps ensure you find both optical and PCR duplicates.
1. After what steps should duplicates be marked a second time?
I understand it may depend on the variant discovery workflow being followed but it would be helpful to have more specific guidance as a second step marking duplicates is not mentioned in any of the following:
In addition, in the Data pre-processing for variant discovery tutorial, only marking duplicates per sample is described.
2. What are the limitations of marking duplicates only once, such as only once files are aggregated from the same sample from across lanes?
Thank you for your time and help.
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