Genome Analysis Toolkit

Variant Discovery in High-Throughput Sequencing Data

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Developed in the Data Sciences Platform at the Broad Institute, the toolkit offers a wide variety of tools with a primary focus on variant discovery and genotyping. Its powerful processing engine and high-performance computing features make it capable of taking on projects of any size. Learn more

Read groups: How do I assign RGID when all samples in a pool are run on all available lanes on a flow cell?

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    Genevieve Brandt

    ISmolicz each ID must be unique for each read group, it does not necessarily need to be named with the flowcell. 

    For example, this read group:

    @RG ID:H0164.2  PL:illumina PU:H0164ALXX140820.2    LB:Solexa-272222    PI:0    DT:2014-08-20T00:00:00-0400 SM:NA12878  CN:BI

     

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    ISmolicz

    Thank you for confirming Genevieve Brandt. I will ensure the RGID is unique per read group.

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