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Variant Discovery in High-Throughput Sequencing Data

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Developed in the Data Sciences Platform at the Broad Institute, the toolkit offers a wide variety of tools with a primary focus on variant discovery and genotyping. Its powerful processing engine and high-performance computing features make it capable of taking on projects of any size. Learn more

Error: Unable to load from native/ (No space left on device)



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    Genevieve Brandt

    Hello Alex Sanchez, is there room on your machine where you are running this command?

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    Alex Sanchez

    Hello, Thanks for your answer. I usually submit this job to a Load Sharing Facility platform using the command bsub. I have 2TB storage space in this platform and I am only using 25% of it, so far other jobs such as alignment using bwa algorithm and marking duplicates with picard worked well. I am also reaching to my IT department so they can let me know if there is any space issues but as far as I know, in the past, other jobs run well.

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    Genevieve Brandt

    Alex Sanchez try using the GATK wrapper script to submit your command, there can be weird errors when using the jar file. [More info here]

    Also, you may want to specify a temp directory with the option --tmp-dir

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    Alex Sanchez

    Hello, thanks for your response. The problem was solved by uninstalling and older version of gatk and also running GATK wrapper script. But now I am wondering if gatk also uses samtools? cause now I have another error when trying to run the pipeline:

    htsjdk.samtools.SAMException: Sequence name '' doesn't match regex: '[0-9A-Za-z!#$%&+./:;?@^_|~-][0-9A-Za-z!#$%&*+./:;=?@^_|~-]*'

    This may be related to samtools tho, but any feedback is appreciated. Thanks!



    gatk --java-options "-Xmx10g" BaseRecalibrator -I T_HG2.mkdup.bam -R ~/reference_genome/hg38/GRCh38_full_analysis_set_plus_decoy_hla.fa --known-sites ~/recalibration/dbSNP154v2/sorted.dbSNP154.vcf.gz -O T_HG2.recal_data.grp

    Using GATK jar /nethome/axs2785/miniconda3/envs/align/share/gatk4-


        java -Dsamjdk.use_async_io_read_samtools=false -Dsamjdk.use_async_io_write_samtools=true -Dsamjdk.use_async_io_write_tribble=false -Dsamjdk.compression_level=2 -Xmx10g -jar /nethome/axs2785/miniconda3/envs/align/share/gatk4- BaseRecalibrator -I T_HG2.mkdup.bam -R /nethome/axs2785/reference_genome/hg38/GRCh38_full_analysis_set_plus_decoy_hla.fa --known-sites /nethome/axs2785/recalibration/dbSNP154v2/sorted.dbSNP154.vcf.gz -O T_HG2.recal_data.grp

    Picked up _JAVA_OPTIONS: -Xmx512 -Xmx10g

    13:08:53.832 INFO  NativeLibraryLoader - Loading from jar:file:/nethome/axs2785/miniconda3/envs/align/share/gatk4-!/com/intel/gkl/native/

    Oct 08, 2020 1:08:54 PM runningOnComputeEngine

    INFO: Failed to detect whether we are running on Google Compute Engine.

    13:08:54.017 INFO  BaseRecalibrator - ------------------------------------------------------------

    13:08:54.017 INFO  BaseRecalibrator - The Genome Analysis Toolkit (GATK) v4.1.8.1

    13:08:54.017 INFO  BaseRecalibrator - For support and documentation go to

    13:08:54.018 INFO  BaseRecalibrator - Executing as axs2785@login3 on Linux v2.6.32-431.el6.x86_64 amd64

    13:08:54.018 INFO  BaseRecalibrator - Java runtime: Java HotSpot(TM) 64-Bit Server VM v1.8.0_60-b27

    13:08:54.018 INFO  BaseRecalibrator - Start Date/Time: October 8, 2020 1:08:53 PM EDT

    13:08:54.018 INFO  BaseRecalibrator - ------------------------------------------------------------

    13:08:54.018 INFO  BaseRecalibrator - ------------------------------------------------------------

    13:08:54.018 INFO  BaseRecalibrator - HTSJDK Version: 2.23.0

    13:08:54.018 INFO  BaseRecalibrator - Picard Version: 2.22.8

    13:08:54.018 INFO  BaseRecalibrator - HTSJDK Defaults.COMPRESSION_LEVEL : 2

    13:08:54.019 INFO  BaseRecalibrator - HTSJDK Defaults.USE_ASYNC_IO_READ_FOR_SAMTOOLS : false

    13:08:54.019 INFO  BaseRecalibrator - HTSJDK Defaults.USE_ASYNC_IO_WRITE_FOR_SAMTOOLS : true

    13:08:54.019 INFO  BaseRecalibrator - HTSJDK Defaults.USE_ASYNC_IO_WRITE_FOR_TRIBBLE : false

    13:08:54.019 INFO  BaseRecalibrator - Deflater: IntelDeflater

    13:08:54.019 INFO  BaseRecalibrator - Inflater: IntelInflater

    13:08:54.019 INFO  BaseRecalibrator - GCS max retries/reopens: 20

    13:08:54.019 INFO  BaseRecalibrator - Requester pays: disabled

    13:08:54.019 INFO  BaseRecalibrator - Initializing engine

    13:08:54.608 INFO  FeatureManager - Using codec VCFCodec to read file file:///nethome/axs2785/recalibration/dbSNP154v2/sorted.dbSNP154.vcf.gz

    13:08:54.660 INFO  BaseRecalibrator - Shutting down engine

    [October 8, 2020 1:08:54 PM EDT] done. Elapsed time: 0.01 minutes.


    htsjdk.samtools.SAMException: Sequence name '' doesn't match regex: '[0-9A-Za-z!#$%&+./:;?@^_|~-][0-9A-Za-z!#$%&*+./:;=?@^_|~-]*'

    at htsjdk.samtools.SAMSequenceRecord.validateSequenceName(

    at htsjdk.samtools.SAMSequenceRecord.<init>(

    at htsjdk.variant.vcf.VCFContigHeaderLine.getSAMSequenceRecord(

    at htsjdk.variant.vcf.VCFHeader.getSequenceDictionary(

    at org.broadinstitute.hellbender.engine.FeatureDataSource.getSequenceDictionary(

    at org.broadinstitute.hellbender.engine.FeatureManager.lambda$getAllSequenceDictionaries$6(


    at java.util.Iterator.forEachRemaining(

    at java.util.Spliterators$IteratorSpliterator.forEachRemaining(






    at org.broadinstitute.hellbender.engine.FeatureManager.getAllSequenceDictionaries(

    at org.broadinstitute.hellbender.engine.GATKTool.validateSequenceDictionaries(

    at org.broadinstitute.hellbender.engine.GATKTool.onStartup(

    at org.broadinstitute.hellbender.engine.ReadWalker.onStartup(

    at org.broadinstitute.hellbender.cmdline.CommandLineProgram.runTool(

    at org.broadinstitute.hellbender.cmdline.CommandLineProgram.instanceMainPostParseArgs(

    at org.broadinstitute.hellbender.cmdline.CommandLineProgram.instanceMain(

    at org.broadinstitute.hellbender.Main.runCommandLineProgram(

    at org.broadinstitute.hellbender.Main.mainEntry(

    at org.broadinstitute.hellbender.Main.main(

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  • Avatar
    Genevieve Brandt

    Hi Alex Sanchez, glad it is now working! Please post this issue in a new post since it is unrelated to your first issue, we should be able to look into it.

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