Genome Analysis Toolkit

Variant Discovery in High-Throughput Sequencing Data

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Developed in the Data Sciences Platform at the Broad Institute, the toolkit offers a wide variety of tools with a primary focus on variant discovery and genotyping. Its powerful processing engine and high-performance computing features make it capable of taking on projects of any size. Learn more

HaplotypeCaller--Exception in thread "main" java.lang.OutOfMemoryError: Java Heap Size. Allele -Specific- Annotation

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    Genevieve Brandt (she/her)

    Hi Yangyxt, I noticed you posted two very similar questions. Have you solved one of them? What is your main issue to solve first?

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    Yangyxt

    Hi Brandt,

    Thanks for your response. I did two things to work this thing out:

    1. I increase the Xmx to 75Gb.

    2. I increase the activity-probability-threshold to 0.0015.

     

    I'm not sure which part exactly helps solve the issue. I guess the second operation matters more since I don't see the log records "Path failed to extend was junction tree" anymore. 

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    Genevieve Brandt (she/her)

    Hi Yangyxt, thank you for posting the solution for the benefit of the community! Glad you solved this issue.

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