Genome Analysis Toolkit

Variant Discovery in High-Throughput Sequencing Data

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Developed in the Data Sciences Platform at the Broad Institute, the toolkit offers a wide variety of tools with a primary focus on variant discovery and genotyping. Its powerful processing engine and high-performance computing features make it capable of taking on projects of any size. Learn more

IlluminaBasecallsToSam - barcode was not expected


1 comment

  • Official comment
    Anton Kovalsky

    Hi Vincent Hahaut thanks for your question.

    It looks like you may be missing the barcode in LIBRARYPARAM_SAMPLESHEET.

    The LIBRARYPARAM_SAMPLESHEET needs to have the barcodes associated with the reads in the input. This is required so that the tool can assign the appropriate sample names to the reads based on the barcodes in the LIBRARYPARAM_SAMPLESHEET.

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