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GATK 4.1.7.0 VariantAnnotator Processing Speed Drop

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    Bhanu Gandham

    That's great! And thanks for the update Louis Bergelson

     

    And thank you danilovkiri for bringing this to our attention!

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    Bhanu Gandham

    Hi danilovkiri

     

    Does the process complete at all or does it run infinitely?

    We think that it only seems like the tool is slowing down but it is not actually doing that. The tool skipped variants and started at locus chr21:5011000. It is using the skipped variants in the rate calculations, which is incorrect. This is causing the apparent reduction in speed. It is misleading of course but probably not a serious issue if the tool is running to completion.

    We have created a ticket here to fix this issue and you can follow its progress here: https://github.com/broadinstitute/gatk/issues/6631

     

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    danilovkiri

    Hi Bhanu Gandham

    Unfortunately, I can't answer the first question as I simply have been waiting for quite some time. And there is an issue here: I used to run annotation with VariantAnnotator from GATK3.8. GATK3.8 VariantAnnotator successfully annotated the same input VCF file for chromosomes 21 and 22 within 10 minutes. At the same time, GATK4.1.7.0 VariantAnnotator is somewhere at chr21:5087001 after 10 min or running. It nearly processed nothing compared to GATK3.8. Note that I did not use parallelization.

    I know that GATK3.8 is not supported (but thankfully it still works), though the latest version's behaviour is really strange. 

    I'll run some tests again to compare performance and come back to you.

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    danilovkiri

    Hi Bhanu Gandham

    I run the test again. I used a single-sample indexed VCF for chromosomes chr21 and chr22 to run VariantAnnotator in GATK3.8 and GATK4.1.7.0.

    The command to run GATK3.8 is shown below:

    java -jar -Djava.io.tmpdir=/mnt/disk2/TMP -Xmx30G /home/wgs/Tools/GATK_3.8/GenomeAnalysisTK.jar \
    -T VariantAnnotator -R /home/wgs/Genomes/hg38/bwa/hg38.fa --variant ./barcode.raw21-22.vcf.gz \
    --dbsnp /home/wgs/Tools/Supplementary/dbsnp-153-hgvs-atlasids.sorted.split.hg38.vcf.gz -nt 1 \
    --out ./barcode.annotated.raw21-22.vcf.gz -L chr21 -L chr22

    The command to run GATK4.1.7.0 is as follows:

    /home/wgs/Tools/gatk-4.1.7.0/gatk --java-options "-Xmx30G -Xms1G" \
    VariantAnnotator --reference /home/wgs/Genomes/hg38/bwa/hg38.fa \
    --dbsnp /home/wgs/Tools/Supplementary/dbsnp-153-hgvs-atlasids.sorted.split.hg38.vcf.gz \
    --variant ./barcode.raw21-22.vcf.gz --output ./barcode.annotated_gatk4.raw21-22.vcf.gz \
    -L chr21 -L

    GATK3.8 VariantAnnotator successfully completed annotation within 10 min. The log is shown below:

    INFO 19:27:32,846 HelpFormatter - ------------------------------------------------------------------------------------
    INFO 19:27:32,847 HelpFormatter - The Genome Analysis Toolkit (GATK) v3.8-1-0-gf15c1c3ef, Compiled 2018/02/19 05:43:50
    INFO 19:27:32,847 HelpFormatter - Copyright (c) 2010-2016 The Broad Institute
    INFO 19:27:32,847 HelpFormatter - For support and documentation go to https://software.broadinstitute.org/gatk
    INFO 19:27:32,847 HelpFormatter - [Tue Jun 02 19:27:32 MSK 2020] Executing on Linux 4.15.0-99-generic amd64
    INFO 19:27:32,847 HelpFormatter - Java HotSpot(TM) 64-Bit Server VM 1.8.0_201-b09
    INFO 19:27:32,849 HelpFormatter - Program Args: -T VariantAnnotator -R /home/wgs/Genomes/hg38/bwa/hg38.fa --variant ./barcode.raw21-22.vcf.gz --dbsnp /home/wgs/Tools/Supplementary/dbsnp-153-hgvs-atlasids.sorted.split.hg38.vcf.gz -nt 1 --out ./barcode.annotated.raw21-22.vcf.gz -L chr21 -L chr22
    INFO 19:27:32,850 HelpFormatter - Executing as wgs@wgs on Linux 4.15.0-99-generic amd64; Java HotSpot(TM) 64-Bit Server VM 1.8.0_201-b09.
    INFO 19:27:32,851 HelpFormatter - Date/Time: 2020/06/02 19:27:32
    INFO 19:27:32,851 HelpFormatter - ------------------------------------------------------------------------------------
    INFO 19:27:32,851 HelpFormatter - ------------------------------------------------------------------------------------
    INFO 19:27:32,863 NativeLibraryLoader - Loading libgkl_compression.so from jar:file:/home/wgs/Tools/GATK_3.8/GenomeAnalysisTK.jar!/com/intel/gkl/native/libgkl_compression.so
    INFO 19:27:32,871 GenomeAnalysisEngine - Deflater: IntelDeflater
    INFO 19:27:32,871 GenomeAnalysisEngine - Inflater: IntelInflater
    INFO 19:27:32,871 GenomeAnalysisEngine - Strictness is SILENT
    INFO 19:27:33,679 GenomeAnalysisEngine - Downsampling Settings: Method: BY_SAMPLE, Target Coverage: 250
    INFO 19:27:34,389 IntervalUtils - Processing 97528451 bp from intervals
    WARN 19:27:34,390 IndexDictionaryUtils - Track variant doesn't have a sequence dictionary built in, skipping dictionary validation
    WARN 19:27:34,390 IndexDictionaryUtils - Track dbsnp doesn't have a sequence dictionary built in, skipping dictionary validation
    INFO 19:27:34,419 GenomeAnalysisEngine - Preparing for traversal
    INFO 19:27:34,420 GenomeAnalysisEngine - Done preparing for traversal
    INFO 19:27:34,420 ProgressMeter - [INITIALIZATION COMPLETE; STARTING PROCESSING]
    INFO 19:27:34,420 ProgressMeter - | processed | time | per 1M | | total | remaining
    INFO 19:27:34,420 ProgressMeter - Location | sites | elapsed | sites | completed | runtime | runtime
    INFO 19:28:04,421 ProgressMeter - chr21:9028901 9000000.0 30.0 s 3.0 s 9.3% 5.4 m 4.9 m
    INFO 19:28:34,422 ProgressMeter - chr21:14765701 1.4E7 60.0 s 4.0 s 15.1% 6.6 m 5.6 m
    INFO 19:29:14,423 ProgressMeter - chr21:20002201 2.0E7 100.0 s 5.0 s 20.5% 8.1 m 6.5 m
    INFO 19:29:54,423 ProgressMeter - chr21:25356201 2.5E7 2.3 m 5.0 s 26.0% 9.0 m 6.6 m
    INFO 19:30:24,424 ProgressMeter - chr21:29303601 2.9E7 2.8 m 5.0 s 30.0% 9.4 m 6.6 m
    INFO 19:31:04,424 ProgressMeter - chr21:34687201 3.4E7 3.5 m 6.0 s 35.6% 9.8 m 6.3 m
    INFO 19:31:34,425 ProgressMeter - chr21:38644201 3.8E7 4.0 m 6.0 s 39.6% 10.1 m 6.1 m
    INFO 19:32:14,426 ProgressMeter - chr21:43986501 4.3E7 4.7 m 6.0 s 45.1% 10.3 m 5.7 m
    INFO 19:32:54,426 ProgressMeter - chr22:10108401 5.6709983E7 5.3 m 5.0 s 58.3% 9.2 m 3.8 m
    INFO 19:33:24,427 ProgressMeter - chr22:16711701 6.2709983E7 5.8 m 5.0 s 65.0% 9.0 m 3.1 m
    INFO 19:34:04,427 ProgressMeter - chr22:22250201 6.8709983E7 6.5 m 5.0 s 70.7% 9.2 m 2.7 m
    INFO 19:34:34,428 ProgressMeter - chr22:26324601 7.2709983E7 7.0 m 5.0 s 74.9% 9.3 m 2.3 m
    INFO 19:35:14,428 ProgressMeter - chr22:31496901 7.7709983E7 7.7 m 5.0 s 80.2% 9.6 m 113.0 s
    INFO 19:35:44,430 ProgressMeter - chr22:35375301 8.1709983E7 8.2 m 5.0 s 84.2% 9.7 m 92.0 s
    INFO 19:36:14,431 ProgressMeter - chr22:39382301 8.5709983E7 8.7 m 6.0 s 88.3% 9.8 m 69.0 s
    INFO 19:36:54,431 ProgressMeter - chr22:44578501 9.0709983E7 9.3 m 6.0 s 93.6% 10.0 m 38.0 s
    INFO 19:37:24,432 ProgressMeter - chr22:48423901 9.4709983E7 9.8 m 6.0 s 97.5% 10.1 m 14.0 s
    INFO 19:37:43,505 VariantAnnotator - Processed 97525111 loci.

    INFO 19:37:43,521 ProgressMeter - done 9.7528451E7 10.2 m 6.0 s 100.0% 10.2 m 0.0 s
    INFO 19:37:43,522 ProgressMeter - Total runtime 609.10 secs, 10.15 min, 0.17 hours
    ------------------------------------------------------------------------------------------
    Done. There were 2 WARN messages, the first 2 are repeated below.
    WARN 19:27:34,390 IndexDictionaryUtils - Track variant doesn't have a sequence dictionary built in, skipping dictionary validation
    WARN 19:27:34,390 IndexDictionaryUtils - Track dbsnp doesn't have a sequence dictionary built in, skipping dictionary validation

    GATK4.1.7.0 did not complete annotation within 295 min. I did not decide to wait as it seemed like it was going to take an eternity of CPU time which I was not going to pay for. The available log is shown below:

    Using GATK jar /home/wgs/Tools/gatk-4.1.7.0/gatk-package-4.1.7.0-local.jar
    Running:
    java -Dsamjdk.use_async_io_read_samtools=false -Dsamjdk.use_async_io_write_samtools=true -Dsamjdk.use_async_io_write_tribble=false -Dsamjdk.compression_level=2 -Xmx30G -Xms1G -jar /home/wgs/Tools/gatk-4.1.7.0/gatk-package-4.1.7.0-local.jar VariantAnnotator --reference /home/wgs/Genomes/hg38/bwa/hg38.fa --dbsnp /home/wgs/Tools/Supplementary/dbsnp-153-hgvs-atlasids.sorted.split.hg38.vcf.gz --variant ./barcode.raw21-22.vcf.gz --output ./barcode.annotated_gatk4.raw21-22.vcf.gz -L chr21 -L chr22
    19:57:35.768 INFO NativeLibraryLoader - Loading libgkl_compression.so from jar:file:/home/wgs/Tools/gatk-4.1.7.0/gatk-package-4.1.7.0-local.jar!/com/intel/gkl/native/libgkl_compression.so
    Jun 02, 2020 7:57:35 PM shaded.cloud_nio.com.google.auth.oauth2.ComputeEngineCredentials runningOnComputeEngine
    INFO: Failed to detect whether we are running on Google Compute Engine.
    19:57:35.893 INFO VariantAnnotator - ------------------------------------------------------------
    19:57:35.893 INFO VariantAnnotator - The Genome Analysis Toolkit (GATK) v4.1.7.0
    19:57:35.893 INFO VariantAnnotator - For support and documentation go to https://software.broadinstitute.org/gatk/
    19:57:35.893 INFO VariantAnnotator - Executing as wgs@wgs on Linux v4.15.0-99-generic amd64
    19:57:35.893 INFO VariantAnnotator - Java runtime: Java HotSpot(TM) 64-Bit Server VM v1.8.0_201-b09
    19:57:35.893 INFO VariantAnnotator - Start Date/Time: June 2, 2020 7:57:35 PM MSK
    19:57:35.893 INFO VariantAnnotator - ------------------------------------------------------------
    19:57:35.893 INFO VariantAnnotator - ------------------------------------------------------------
    19:57:35.894 INFO VariantAnnotator - HTSJDK Version: 2.21.2
    19:57:35.894 INFO VariantAnnotator - Picard Version: 2.21.9
    19:57:35.894 INFO VariantAnnotator - HTSJDK Defaults.COMPRESSION_LEVEL : 2
    19:57:35.894 INFO VariantAnnotator - HTSJDK Defaults.USE_ASYNC_IO_READ_FOR_SAMTOOLS : false
    19:57:35.894 INFO VariantAnnotator - HTSJDK Defaults.USE_ASYNC_IO_WRITE_FOR_SAMTOOLS : true
    19:57:35.894 INFO VariantAnnotator - HTSJDK Defaults.USE_ASYNC_IO_WRITE_FOR_TRIBBLE : false
    19:57:35.894 INFO VariantAnnotator - Deflater: IntelDeflater
    19:57:35.894 INFO VariantAnnotator - Inflater: IntelInflater
    19:57:35.894 INFO VariantAnnotator - GCS max retries/reopens: 20
    19:57:35.894 INFO VariantAnnotator - Requester pays: disabled
    19:57:35.894 INFO VariantAnnotator - Initializing engine
    19:57:36.143 INFO FeatureManager - Using codec VCFCodec to read file file:///home/wgs/Tools/Supplementary/dbsnp-153-hgvs-atlasids.sorted.split.hg38.vcf.gz
    19:57:36.213 INFO FeatureManager - Using codec VCFCodec to read file file:///home/wgs/Miscellaneous/gatk_varann_bug_report/./barcode.raw21-22.vcf.gz
    19:57:36.272 INFO IntervalArgumentCollection - Processing 97528451 bp from intervals
    19:57:36.288 INFO VariantAnnotator - Done initializing engine
    19:57:36.337 INFO ProgressMeter - Starting traversal
    19:57:36.337 INFO ProgressMeter - Current Locus Elapsed Minutes Variants Processed Variants/Minute
    19:57:53.125 INFO ProgressMeter - chr21:5011000 0.3 5011000 17910287.7
    19:58:08.400 INFO ProgressMeter - chr21:5013000 0.5 5013000 9380906.3
    19:58:23.621 INFO ProgressMeter - chr21:5015000 0.8 5015000 6363674.8
    19:58:38.749 INFO ProgressMeter - chr21:5017000 1.0 5017000 4823110.9
    19:58:53.813 INFO ProgressMeter - chr21:5019000 1.3 5019000 3886931.3
    19:59:08.872 INFO ProgressMeter - chr21:5021000 1.5 5021000 3255633.0
    19:59:24.138 INFO ProgressMeter - chr21:5023000 1.8 5023000 2795706.9
    19:59:39.010 INFO ProgressMeter - chr21:5025000 2.0 5025000 2457753.5
    19:59:54.037 INFO ProgressMeter - chr21:5027000 2.3 5027000 2190429.9
    20:00:09.082 INFO ProgressMeter - chr21:5029000 2.5 5029000 1975449.3
    20:00:24.173 INFO ProgressMeter - chr21:5031000 2.8 5031000 1798552.1
    20:00:39.274 INFO ProgressMeter - chr21:5033000 3.0 5033000 1650732.2
    20:00:54.478 INFO ProgressMeter - chr21:5035000 3.3 5035000 1524671.8
    20:01:09.551 INFO ProgressMeter - chr21:5037000 3.6 5037000 1417449.1
    20:01:24.606 INFO ProgressMeter - chr21:5039000 3.8 5039000 1324495.8
    20:01:39.722 INFO ProgressMeter - chr21:5041000 4.1 5041000 1242722.4
    20:01:54.788 INFO ProgressMeter - chr21:5043000 4.3 5043000 1170744.2
    20:02:09.836 INFO ProgressMeter - chr21:5045000 4.6 5045000 1106768.2
    20:02:24.871 INFO ProgressMeter - chr21:5047000 4.8 5047000 1049512.4
    20:02:39.892 INFO ProgressMeter - chr21:5049000 5.1 5049000 997974.0
    20:02:54.936 INFO ProgressMeter - chr21:5051000 5.3 5051000 951227.1
    20:03:09.993 INFO ProgressMeter - chr21:5053000 5.6 5053000 908660.4
    20:03:25.023 INFO ProgressMeter - chr21:5055000 5.8 5055000 869837.0
    20:03:40.066 INFO ProgressMeter - chr21:5057000 6.1 5057000 834192.5
    20:03:55.089 INFO ProgressMeter - chr21:5059000 6.3 5059000 801421.5
    20:04:10.146 INFO ProgressMeter - chr21:5061000 6.6 5061000 771084.5
    20:04:25.165 INFO ProgressMeter - chr21:5063000 6.8 5063000 743050.9
    20:04:40.216 INFO ProgressMeter - chr21:5065000 7.1 5065000 716949.9
    20:04:55.216 INFO ProgressMeter - chr21:5067000 7.3 5067000 692719.4
    20:05:10.268 INFO ProgressMeter - chr21:5069000 7.6 5069000 670013.7
    20:05:25.371 INFO ProgressMeter - chr21:5071001 7.8 5071000 648695.0
    20:05:40.340 INFO ProgressMeter - chr21:5073001 8.1 5073000 628880.4
    20:05:55.393 INFO ProgressMeter - chr21:5075001 8.3 5075000 610152.0
    20:06:10.446 INFO ProgressMeter - chr21:5077001 8.6 5077000 592520.3
    20:06:25.520 INFO ProgressMeter - chr21:5079001 8.8 5079000 575868.8
    20:06:40.574 INFO ProgressMeter - chr21:5081001 9.1 5081000 560160.4
    20:06:55.635 INFO ProgressMeter - chr21:5083001 9.3 5083000 545290.7
    20:07:10.679 INFO ProgressMeter - chr21:5085001 9.6 5085000 531216.6
    20:07:25.755 INFO ProgressMeter - chr21:5087001 9.8 5087000 517832.8
    20:07:40.835 INFO ProgressMeter - chr21:5089001 10.1 5089000 505114.2
    20:07:55.922 INFO ProgressMeter - chr21:5091001 10.3 5091000 493007.4
    20:08:10.987 INFO ProgressMeter - chr21:5093001 10.6 5093000 481493.7
    20:08:26.072 INFO ProgressMeter - chr21:5095001 10.8 5095000 470499.5
    20:08:41.144 INFO ProgressMeter - chr21:5097001 11.1 5097000 460013.2
    20:08:56.257 INFO ProgressMeter - chr21:5099001 11.3 5099000 449964.7
    20:09:11.414 INFO ProgressMeter - chr21:5101001 11.6 5101000 440325.3
    20:09:26.441 INFO ProgressMeter - chr21:5103001 11.8 5103000 431176.3
    20:09:41.454 INFO ProgressMeter - chr21:5105002 12.1 5105000 422414.6
    20:09:56.452 INFO ProgressMeter - chr21:5107002 12.3 5107000 414016.7
    20:10:11.690 INFO ProgressMeter - chr21:5109002 12.6 5109000 405823.5
    20:10:26.902 INFO ProgressMeter - chr21:5111002 12.8 5111000 397967.7
    20:10:42.087 INFO ProgressMeter - chr21:5113002 13.1 5113000 390429.5
    20:10:57.173 INFO ProgressMeter - chr21:5115002 13.3 5115000 383224.5
    20:11:12.352 INFO ProgressMeter - chr21:5117002 13.6 5117000 376243.1
    20:11:27.473 INFO ProgressMeter - chr21:5119002 13.9 5119000 369542.4
    20:11:42.537 INFO ProgressMeter - chr21:5121002 14.1 5121000 363105.6
    20:11:57.599 INFO ProgressMeter - chr21:5123002 14.4 5123000 356894.9
    20:12:12.687 INFO ProgressMeter - chr21:5125002 14.6 5125000 350887.2
    20:12:27.581 INFO ProgressMeter - chr21:5127002 14.9 5127000 345158.0
    20:12:42.378 INFO ProgressMeter - chr21:5129002 15.1 5129000 339653.5
    20:12:57.438 INFO ProgressMeter - chr21:5131002 15.4 5131000 334230.4
    20:13:12.573 INFO ProgressMeter - chr21:5133002 15.6 5133000 328955.5
    20:13:27.846 INFO ProgressMeter - chr21:5135002 15.9 5135000 323801.5
    20:13:42.879 INFO ProgressMeter - chr21:5137002 16.1 5137000 318889.4
    20:13:57.936 INFO ProgressMeter - chr21:5139002 16.4 5139000 314120.1
    20:14:13.005 INFO ProgressMeter - chr21:5141002 16.6 5141000 309491.2
    20:14:28.086 INFO ProgressMeter - chr21:5143002 16.9 5143000 304996.6
    20:14:43.129 INFO ProgressMeter - chr21:5145002 17.1 5145000 300645.1
    20:14:58.196 INFO ProgressMeter - chr21:5147002 17.4 5147000 296412.5
    20:15:13.245 INFO ProgressMeter - chr21:5149002 17.6 5149000 292305.8
    20:15:28.339 INFO ProgressMeter - chr21:5151002 17.9 5151000 288301.7
    20:15:43.434 INFO ProgressMeter - chr21:5153002 18.1 5153000 284408.8
    20:15:58.501 INFO ProgressMeter - chr21:5155002 18.4 5155000 280629.7
    20:16:13.597 INFO ProgressMeter - chr21:5157002 18.6 5157000 276945.4
    20:16:28.704 INFO ProgressMeter - chr21:5159002 18.9 5159000 273356.8
    20:16:43.775 INFO ProgressMeter - chr21:5161002 19.1 5161000 269871.0
    20:16:58.777 INFO ProgressMeter - chr21:5163002 19.4 5163000 266491.2
    20:17:13.839 INFO ProgressMeter - chr21:5165002 19.6 5165000 263184.3
    20:17:29.080 INFO ProgressMeter - chr21:5217002 19.9 5217000 262437.1
    20:17:44.128 INFO ProgressMeter - chr21:5219003 20.1 5219000 259266.7
    20:17:59.150 INFO ProgressMeter - chr21:5221004 20.4 5221000 256179.8
    20:18:14.220 INFO ProgressMeter - chr21:5223004 20.6 5223000 253158.0
    20:18:29.287 INFO ProgressMeter - chr21:5225004 20.9 5225000 250209.5
    20:18:44.357 INFO ProgressMeter - chr21:5227004 21.1 5227000 247330.5
    20:18:59.465 INFO ProgressMeter - chr21:5229005 21.4 5229000 244511.8
    20:19:14.539 INFO ProgressMeter - chr21:5231008 21.6 5231000 241765.1
    20:19:29.570 INFO ProgressMeter - chr21:5233009 21.9 5233000 239089.3
    20:19:44.649 INFO ProgressMeter - chr21:5235010 22.1 5235000 236465.5
    20:19:59.737 INFO ProgressMeter - chr21:5237010 22.4 5237000 233899.1
    20:20:14.822 INFO ProgressMeter - chr21:5239010 22.6 5239000 231390.1
    20:20:29.872 INFO ProgressMeter - chr21:5241010 22.9 5241000 228942.1
    20:20:44.937 INFO ProgressMeter - chr21:5243012 23.1 5243000 226544.7
    20:20:59.933 INFO ProgressMeter - chr21:5245012 23.4 5245000 224209.8
    20:21:14.876 INFO ProgressMeter - chr21:5247012 23.6 5247000 221932.6
    20:21:29.928 INFO ProgressMeter - chr21:5249014 23.9 5249000 219686.1
    20:21:44.963 INFO ProgressMeter - chr21:5251015 24.1 5251000 217488.8
    20:22:00.037 INFO ProgressMeter - chr21:5253016 24.4 5253000 215331.0
    20:22:15.115 INFO ProgressMeter - chr21:5255016 24.6 5255000 213216.6
    20:22:30.200 INFO ProgressMeter - chr21:5257016 24.9 5257000 211144.0
    20:22:45.290 INFO ProgressMeter - chr21:5259016 25.1 5259000 209112.0
    20:23:00.349 INFO ProgressMeter - chr21:5261016 25.4 5261000 207124.4
    20:23:15.480 INFO ProgressMeter - chr21:5263016 25.7 5263000 205166.1
    20:23:30.497 INFO ProgressMeter - chr21:5265020 25.9 5265000 203260.9
    20:23:45.598 INFO ProgressMeter - chr21:5267020 26.2 5267000 201381.4
    20:24:00.668 INFO ProgressMeter - chr21:5269020 26.4 5269000 199541.6
    20:24:15.841 INFO ProgressMeter - chr21:5271020 26.7 5271000 197723.8
    20:24:30.785 INFO ProgressMeter - chr21:5273021 26.9 5273000 195967.9
    20:24:45.749 INFO ProgressMeter - chr21:5275021 27.2 5275000 194241.8
    20:25:00.685 INFO ProgressMeter - chr21:5277021 27.4 5277000 192550.5
    20:25:15.615 INFO ProgressMeter - chr21:5279022 27.7 5279000 190890.3
    20:25:30.568 INFO ProgressMeter - chr21:5281022 27.9 5281000 189257.2
    20:25:45.547 INFO ProgressMeter - chr21:5283024 28.2 5283000 187649.8
    20:26:00.505 INFO ProgressMeter - chr21:5285024 28.4 5285000 186073.2
    20:26:15.524 INFO ProgressMeter - chr21:5287024 28.7 5287000 184517.4
    20:26:30.499 INFO ProgressMeter - chr21:5289024 28.9 5289000 182993.4
    20:26:45.543 INFO ProgressMeter - chr21:5291026 29.2 5291000 181488.1
    20:27:00.528 INFO ProgressMeter - chr21:5293026 29.4 5293000 180014.5
    20:27:15.509 INFO ProgressMeter - chr21:5295026 29.7 5295000 178566.2
    20:27:30.467 INFO ProgressMeter - chr21:5297026 29.9 5297000 177144.4
    20:27:45.419 INFO ProgressMeter - chr21:5299027 30.2 5299000 175746.6
    20:28:00.352 INFO ProgressMeter - chr21:5301027 30.4 5301000 174373.6
    20:28:15.276 INFO ProgressMeter - chr21:5303031 30.6 5303000 173023.7
    20:28:30.221 INFO ProgressMeter - chr21:5305032 30.9 5305000 171693.6
    20:28:45.169 INFO ProgressMeter - chr21:5307033 31.1 5307000 170384.5
    20:29:00.094 INFO ProgressMeter - chr21:5309033 31.4 5309000 169098.2
    20:29:15.042 INFO ProgressMeter - chr21:5311033 31.6 5311000 167830.2
    20:29:29.989 INFO ProgressMeter - chr21:5313033 31.9 5313000 166582.0
    20:29:44.995 INFO ProgressMeter - chr21:5315035 32.1 5315000 165348.1
    20:30:00.147 INFO ProgressMeter - chr21:5317035 32.4 5317000 164121.0
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    21:34:11.970 INFO ProgressMeter - chr21:6078058 96.6 6078000 62923.2
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    21:34:42.066 INFO ProgressMeter - chr21:6082058 97.1 6082000 62639.4
    21:34:57.147 INFO ProgressMeter - chr21:6084058 97.3 6084000 62498.2
    21:35:12.223 INFO ProgressMeter - chr21:6086058 97.6 6086000 62357.8
    21:35:27.266 INFO ProgressMeter - chr21:6088058 97.8 6088000 62218.4
    21:35:42.343 INFO ProgressMeter - chr21:6090058 98.1 6090000 62079.5
    21:35:57.376 INFO ProgressMeter - chr21:6092058 98.4 6092000 61941.6
    21:36:12.539 INFO ProgressMeter - chr21:6094058 98.6 6094000 61803.2
    21:36:27.592 INFO ProgressMeter - chr21:6096058 98.9 6096000 61666.5
    21:36:42.628 INFO ProgressMeter - chr21:6098059 99.1 6098000 61530.8
    21:36:57.641 INFO ProgressMeter - chr21:6100059 99.4 6100000 61396.0
    21:37:12.683 INFO ProgressMeter - chr21:6102059 99.6 6102000 61261.5
    21:37:27.732 INFO ProgressMeter - chr21:6104059 99.9 6104000 61127.7
    21:37:42.770 INFO ProgressMeter - chr21:6106059 100.1 6106000 60994.6
    21:37:57.809 INFO ProgressMeter - chr21:6108059 100.4 6108000 60862.2
    21:38:12.815 INFO ProgressMeter - chr21:6110059 100.6 6110000 60730.8
    21:38:27.844 INFO ProgressMeter - chr21:6112059 100.9 6112000 60599.8
    21:38:42.899 INFO ProgressMeter - chr21:6114059 101.1 6114000 60469.2
    21:38:57.915 INFO ProgressMeter - chr21:6116059 101.4 6116000 60339.6
    21:39:12.929 INFO ProgressMeter - chr21:6118059 101.6 6118000 60210.7
    21:39:27.986 INFO ProgressMeter - chr21:6120059 101.9 6120000 60082.0
    21:39:43.041 INFO ProgressMeter - chr21:6122059 102.1 6122000 59953.9
    21:39:58.093 INFO ProgressMeter - chr21:6124059 102.4 6124000 59826.5
    21:40:13.182 INFO ProgressMeter - chr21:6126060 102.6 6126000 59699.4
    21:40:28.305 INFO ProgressMeter - chr21:6128060 102.9 6128000 59572.6
    21:40:43.399 INFO ProgressMeter - chr21:6130060 103.1 6130000 59446.6
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    21:44:44.704 INFO ProgressMeter - chr21:6212061 107.1 6212000 57980.5
    21:44:59.628 INFO ProgressMeter - chr21:6214061 107.4 6214000 57864.8
    21:45:14.567 INFO ProgressMeter - chr21:6216061 107.6 6216000 57749.6
    21:45:29.538 INFO ProgressMeter - chr21:6218061 107.9 6218000 57634.5
    21:45:44.572 INFO ProgressMeter - chr21:6220061 108.1 6220000 57519.5
    21:45:59.696 INFO ProgressMeter - chr21:6222061 108.4 6222000 57404.2
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    21:46:59.854 INFO ProgressMeter - chr21:6230061 109.4 6230000 56951.2
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    21:48:00.025 INFO ProgressMeter - chr21:6238061 110.4 6238000 56506.3
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    21:48:30.019 INFO ProgressMeter - chr21:6242061 110.9 6242000 56287.6
    21:48:44.997 INFO ProgressMeter - chr21:6244061 111.1 6244000 56179.2
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    21:49:14.784 INFO ProgressMeter - chr21:6248061 111.6 6248000 55965.2
    21:49:29.873 INFO ProgressMeter - chr21:6250061 111.9 6250000 55857.3
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    21:50:00.065 INFO ProgressMeter - chr21:6254061 112.4 6254000 55642.8
    21:50:15.148 INFO ProgressMeter - chr21:6256061 112.6 6256000 55536.4
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    21:50:45.338 INFO ProgressMeter - chr21:6260061 113.2 6260000 55324.8
    21:51:00.369 INFO ProgressMeter - chr21:6262061 113.4 6262000 55220.2
    21:51:15.413 INFO ProgressMeter - chr21:6264061 113.7 6264000 55116.0
    21:51:30.475 INFO ProgressMeter - chr21:6266061 113.9 6266000 55012.1
    21:51:45.513 INFO ProgressMeter - chr21:6268061 114.2 6268000 54908.8
    21:52:00.568 INFO ProgressMeter - chr21:6270061 114.4 6270000 54805.8
    21:52:15.688 INFO ProgressMeter - chr21:6272061 114.7 6272000 54702.8
    21:52:30.798 INFO ProgressMeter - chr21:6274061 114.9 6274000 54600.4
    21:52:45.901 INFO ProgressMeter - chr21:6276061 115.2 6276000 54498.4
    21:53:00.912 INFO ProgressMeter - chr21:6278061 115.4 6278000 54397.6
    21:53:15.976 INFO ProgressMeter - chr21:6280061 115.7 6280000 54296.8
    21:53:30.998 INFO ProgressMeter - chr21:6282061 115.9 6282000 54196.7
    21:53:45.959 INFO ProgressMeter - chr21:6284061 116.2 6284000 54097.6
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    21:54:16.100 INFO ProgressMeter - chr21:6288061 116.7 6288000 53899.0
    21:54:31.123 INFO ProgressMeter - chr21:6290061 116.9 6290000 53800.6
    21:54:46.158 INFO ProgressMeter - chr21:6292061 117.2 6292000 53702.6
    21:55:01.176 INFO ProgressMeter - chr21:6294061 117.4 6294000 53605.2
    21:55:16.207 INFO ProgressMeter - chr21:6296061 117.7 6296000 53508.1
    21:55:31.266 INFO ProgressMeter - chr21:6298061 117.9 6298000 53411.1
    21:55:46.452 INFO ProgressMeter - chr21:6300061 118.2 6300000 53313.7
    21:56:01.613 INFO ProgressMeter - chr21:6302061 118.4 6302000 53216.8
    21:56:16.659 INFO ProgressMeter - chr21:6304061 118.7 6304000 53121.2
    21:56:31.792 INFO ProgressMeter - chr21:6306061 118.9 6306000 53025.4
    21:56:46.802 INFO ProgressMeter - chr21:6308061 119.2 6308000 52930.8
    21:57:01.820 INFO ProgressMeter - chr21:6310061 119.4 6310000 52836.6
    21:57:16.780 INFO ProgressMeter - chr21:6312061 119.7 6312000 52743.3
    21:57:31.763 INFO ProgressMeter - chr21:6314061 119.9 6314000 52650.1
    21:57:46.803 INFO ProgressMeter - chr21:6316061 120.2 6316000 52556.9
    21:58:01.845 INFO ProgressMeter - chr21:6318061 120.4 6318000 52464.1
    21:58:16.906 INFO ProgressMeter - chr21:6320061 120.7 6320000 52371.6
    21:58:31.971 INFO ProgressMeter - chr21:6322061 120.9 6322000 52279.4
    21:58:47.029 INFO ProgressMeter - chr21:6324061 121.2 6324000 52187.6
    21:59:02.076 INFO ProgressMeter - chr21:6326061 121.4 6326000 52096.3
    21:59:17.184 INFO ProgressMeter - chr21:6328061 121.7 6328000 52004.9
    21:59:32.247 INFO ProgressMeter - chr21:6330061 121.9 6330000 51914.3
    21:59:47.298 INFO ProgressMeter - chr21:6332061 122.2 6332000 51824.0
    22:00:02.368 INFO ProgressMeter - chr21:6334061 122.4 6334000 51734.1
    22:00:17.357 INFO ProgressMeter - chr21:6336061 122.7 6336000 51645.0
    22:00:32.346 INFO ProgressMeter - chr21:6338061 122.9 6338000 51556.3
    22:00:47.405 INFO ProgressMeter - chr21:6340061 123.2 6340000 51467.5
    22:01:02.370 INFO ProgressMeter - chr21:6342061 123.4 6342000 51379.7
    22:01:17.346 INFO ProgressMeter - chr21:6344061 123.7 6344000 51292.2
    22:01:32.324 INFO ProgressMeter - chr21:6346061 123.9 6346000 51205.0
    22:01:47.288 INFO ProgressMeter - chr21:6348061 124.2 6348000 51118.3
    22:02:02.291 INFO ProgressMeter - chr21:6350061 124.4 6350000 51031.7
    22:02:17.263 INFO ProgressMeter - chr21:6352061 124.7 6352000 50945.6
    22:02:32.312 INFO ProgressMeter - chr21:6354061 124.9 6354000 50859.3
    22:02:47.372 INFO ProgressMeter - chr21:6356061 125.2 6356000 50773.3
    22:03:02.477 INFO ProgressMeter - chr21:6358061 125.4 6358000 50687.3
    22:03:17.513 INFO ProgressMeter - chr21:6360061 125.7 6360000 50602.2
    22:03:32.539 INFO ProgressMeter - chr21:6362061 125.9 6362000 50517.4
    22:03:47.555 INFO ProgressMeter - chr21:6364061 126.2 6364000 50433.1
    22:04:02.599 INFO ProgressMeter - chr21:6366062 126.4 6366000 50348.9
    22:04:17.659 INFO ProgressMeter - chr21:6368063 126.7 6368000 50264.9
    22:04:32.736 INFO ProgressMeter - chr21:6370064 126.9 6370000 50181.2
    22:04:47.793 INFO ProgressMeter - chr21:6372064 127.2 6372000 50097.9
    22:05:02.852 INFO ProgressMeter - chr21:6374065 127.4 6374000 50014.9
    22:05:17.934 INFO ProgressMeter - chr21:6376066 127.7 6376000 49932.1
    22:05:33.114 INFO ProgressMeter - chr21:6428066 127.9 6428000 50239.8
    22:05:48.166 INFO ProgressMeter - chr21:6430066 128.2 6430000 50157.1
    22:06:03.216 INFO ProgressMeter - chr21:6432066 128.4 6432000 50074.7
    22:06:18.254 INFO ProgressMeter - chr21:6434066 128.7 6434000 49992.8
    22:06:33.312 INFO ProgressMeter - chr21:6436066 128.9 6436000 49911.0
    22:06:48.388 INFO ProgressMeter - chr21:6438066 129.2 6438000 49829.4
    22:07:03.468 INFO ProgressMeter - chr21:6440066 129.5 6440000 49748.1
    22:07:18.508 INFO ProgressMeter - chr21:6442066 129.7 6442000 49667.4
    22:07:33.555 INFO ProgressMeter - chr21:6444066 130.0 6444000 49586.9
    22:07:48.582 INFO ProgressMeter - chr21:6446066 130.2 6446000 49506.9
    22:08:03.621 INFO ProgressMeter - chr21:6448066 130.5 6448000 49427.1
    22:08:18.675 INFO ProgressMeter - chr21:6450066 130.7 6450000 49347.5
    22:08:33.706 INFO ProgressMeter - chr21:6452066 131.0 6452000 49268.4
    22:08:48.749 INFO ProgressMeter - chr21:6454066 131.2 6454000 49189.5
    22:09:03.806 INFO ProgressMeter - chr21:6456066 131.5 6456000 49110.8
    22:09:18.593 INFO ProgressMeter - chr21:6458066 131.7 6458000 49034.1
    22:09:33.505 INFO ProgressMeter - chr21:6460066 132.0 6460000 48956.9
    22:09:48.542 INFO ProgressMeter - chr21:6462066 132.2 6462000 48879.2
    22:10:03.576 INFO ProgressMeter - chr21:6464066 132.5 6464000 48801.9
    22:10:18.617 INFO ProgressMeter - chr21:6466066 132.7 6466000 48724.7
    22:10:33.654 INFO ProgressMeter - chr21:6468066 133.0 6468000 48647.9
    22:10:48.643 INFO ProgressMeter - chr21:6470066 133.2 6470000 48571.7
    22:11:03.584 INFO ProgressMeter - chr21:6472066 133.5 6472000 48496.1
    22:11:18.502 INFO ProgressMeter - chr21:6474066 133.7 6474000 48420.8
    22:11:33.447 INFO ProgressMeter - chr21:6476066 134.0 6476000 48345.7
    22:11:48.358 INFO ProgressMeter - chr21:6478066 134.2 6478000 48271.1
    22:12:03.299 INFO ProgressMeter - chr21:6480066 134.4 6480000 48196.6
    22:12:18.233 INFO ProgressMeter - chr21:6482066 134.7 6482000 48122.4
    22:12:33.194 INFO ProgressMeter - chr21:6484066 134.9 6484000 48048.3
    22:12:48.006 INFO ProgressMeter - chr21:6486066 135.2 6486000 47975.3
    22:13:02.995 INFO ProgressMeter - chr21:6488066 135.4 6488000 47901.6
    22:13:17.976 INFO ProgressMeter - chr21:6490067 135.7 6490000 47828.2
    22:13:33.037 INFO ProgressMeter - chr21:6492068 135.9 6492000 47754.6
    22:13:48.065 INFO ProgressMeter - chr21:6494068 136.2 6494000 47681.5
    22:14:02.871 INFO ProgressMeter - chr21:6496068 136.4 6496000 47609.9
    22:14:17.707 INFO ProgressMeter - chr21:6498068 136.7 6498000 47538.4
    22:14:32.789 INFO ProgressMeter - chr21:6500068 136.9 6500000 47465.7
    22:14:47.899 INFO ProgressMeter - chr21:6502068 137.2 6502000 47393.2
    22:15:02.998 INFO ProgressMeter - chr21:6504068 137.4 6504000 47321.0
    22:15:18.089 INFO ProgressMeter - chr21:6506068 137.7 6506000 47249.1
    22:15:33.129 INFO ProgressMeter - chr21:6508068 137.9 6508000 47177.7
    22:15:48.110 INFO ProgressMeter - chr21:6510068 138.2 6510000 47106.9
    22:16:03.157 INFO ProgressMeter - chr21:6512068 138.4 6512000 47036.0
    22:16:18.196 INFO ProgressMeter - chr21:6514068 138.7 6514000 46965.5
    22:16:33.256 INFO ProgressMeter - chr21:6516068 138.9 6516000 46895.0
    22:16:48.356 INFO ProgressMeter - chr21:6518069 139.2 6518000 46824.6
    22:17:03.412 INFO ProgressMeter - chr21:6520069 139.5 6520000 46754.7
    22:17:18.486 INFO ProgressMeter - chr21:6522069 139.7 6522000 46684.9
    22:17:33.544 INFO ProgressMeter - chr21:6524069 140.0 6524000 46615.5
    22:17:48.603 INFO ProgressMeter - chr21:6526069 140.2 6526000 46546.3
    22:18:03.790 INFO ProgressMeter - chr21:6528069 140.5 6528000 46476.7
    22:18:18.889 INFO ProgressMeter - chr21:6530069 140.7 6530000 46407.8
    22:18:33.969 INFO ProgressMeter - chr21:6532069 141.0 6532000 46339.2
    22:18:49.056 INFO ProgressMeter - chr21:6534069 141.2 6534000 46270.9
    22:19:04.284 INFO ProgressMeter - chr21:6536069 141.5 6536000 46202.0
    22:19:19.500 INFO ProgressMeter - chr21:6538069 141.7 6538000 46133.4
    22:19:34.686 INFO ProgressMeter - chr21:6540069 142.0 6540000 46065.3
    22:19:49.734 INFO ProgressMeter - chr21:6542069 142.2 6542000 45998.1
    22:20:04.776 INFO ProgressMeter - chr21:6544069 142.5 6544000 45931.2
    22:20:19.878 INFO ProgressMeter - chr21:6546069 142.7 6546000 45864.2
    22:20:34.906 INFO ProgressMeter - chr21:6548069 143.0 6548000 45797.8
    22:20:50.109 INFO ProgressMeter - chr21:6550069 143.2 6550000 45730.8
    22:21:05.181 INFO ProgressMeter - chr21:6552069 143.5 6552000 45664.7
    22:21:20.216 INFO ProgressMeter - chr21:6554069 143.7 6554000 45599.0
    22:21:35.260 INFO ProgressMeter - chr21:6556069 144.0 6556000 45533.5
    22:21:50.309 INFO ProgressMeter - chr21:6558069 144.2 6558000 45468.1
    22:22:05.405 INFO ProgressMeter - chr21:6560069 144.5 6560000 45402.8
    22:22:20.487 INFO ProgressMeter - chr21:6562069 144.7 6562000 45337.8
    22:22:35.747 INFO ProgressMeter - chr21:6564069 145.0 6564000 45272.0
    22:22:50.781 INFO ProgressMeter - chr21:6566069 145.2 6566000 45207.7
    22:23:05.839 INFO ProgressMeter - chr21:6568070 145.5 6568000 45143.5
    22:23:21.084 INFO ProgressMeter - chr21:6570070 145.7 6570000 45078.5
    22:23:36.235 INFO ProgressMeter - chr21:6572070 146.0 6572000 45014.2
    22:23:51.505 INFO ProgressMeter - chr21:6574070 146.3 6574000 44949.6
    22:24:07.079 INFO ProgressMeter - chr21:6576070 146.5 6576000 44883.6
    22:24:22.350 INFO ProgressMeter - chr21:6578070 146.8 6578000 44819.4
    22:24:37.703 INFO ProgressMeter - chr21:6580070 147.0 6580000 44755.0
    22:24:52.854 INFO ProgressMeter - chr21:6632070 147.3 6632000 45031.3
    22:25:07.953 INFO ProgressMeter - chr21:6634070 147.5 6634000 44968.1
    22:25:23.082 INFO ProgressMeter - chr21:6636070 147.8 6636000 44904.9
    22:25:38.168 INFO ProgressMeter - chr21:6638070 148.0 6638000 44842.1
    22:25:53.372 INFO ProgressMeter - chr21:6640070 148.3 6640000 44779.0
    22:26:08.499 INFO ProgressMeter - chr21:6642070 148.5 6642000 44716.4
    22:26:23.552 INFO ProgressMeter - chr21:6644070 148.8 6644000 44654.5
    22:26:38.653 INFO ProgressMeter - chr21:6646070 149.0 6646000 44592.5
    22:26:53.721 INFO ProgressMeter - chr21:6648070 149.3 6648000 44530.9
    22:27:08.780 INFO ProgressMeter - chr21:6650070 149.5 6650000 44469.5
    22:27:23.818 INFO ProgressMeter - chr21:6652070 149.8 6652000 44408.4
    22:27:38.876 INFO ProgressMeter - chr21:6654070 150.0 6654000 44347.5
    22:27:53.921 INFO ProgressMeter - chr21:6656070 150.3 6656000 44286.8
    22:28:08.955 INFO ProgressMeter - chr21:6658070 150.5 6658000 44226.4
    22:28:24.020 INFO ProgressMeter - chr21:6660070 150.8 6660000 44166.0
    22:28:38.974 INFO ProgressMeter - chr21:6662070 151.0 6662000 44106.4
    22:28:54.014 INFO ProgressMeter - chr21:6664070 151.3 6664000 44046.5
    22:29:09.042 INFO ProgressMeter - chr21:6666070 151.5 6666000 43986.9
    22:29:24.055 INFO ProgressMeter - chr21:6668070 151.8 6668000 43927.6
    22:29:39.082 INFO ProgressMeter - chr21:6670070 152.0 6670000 43868.4
    22:29:54.107 INFO ProgressMeter - chr21:6672070 152.3 6672000 43809.4
    22:30:09.126 INFO ProgressMeter - chr21:6674070 152.5 6674000 43750.6
    22:30:24.160 INFO ProgressMeter - chr21:6676070 152.8 6676000 43691.9
    22:30:39.193 INFO ProgressMeter - chr21:6678070 153.0 6678000 43633.5
    22:30:54.225 INFO ProgressMeter - chr21:6680070 153.3 6680000 43575.2
    22:31:09.237 INFO ProgressMeter - chr21:6682070 153.5 6682000 43517.2
    22:31:24.288 INFO ProgressMeter - chr21:6684070 153.8 6684000 43459.3
    22:31:39.248 INFO ProgressMeter - chr21:6686070 154.0 6686000 43401.9
    22:31:54.142 INFO ProgressMeter - chr21:6688070 154.3 6688000 43345.0
    22:32:08.944 INFO ProgressMeter - chr21:6690070 154.5 6690000 43288.8
    22:32:23.929 INFO ProgressMeter - chr21:6692070 154.8 6692000 43231.9
    22:32:39.074 INFO ProgressMeter - chr21:6694070 155.0 6694000 43174.4
    22:32:54.146 INFO ProgressMeter - chr21:6696070 155.3 6696000 43117.4
    22:33:09.276 INFO ProgressMeter - chr21:6698070 155.5 6698000 43060.4
    22:33:24.471 INFO ProgressMeter - chr21:6700070 155.8 6700000 43003.2
    22:33:39.607 INFO ProgressMeter - chr21:6702070 156.1 6702000 42946.5
    22:33:54.646 INFO ProgressMeter - chr21:6704070 156.3 6704000 42890.5
    22:34:09.804 INFO ProgressMeter - chr21:6706070 156.6 6706000 42834.0
    22:34:24.832 INFO ProgressMeter - chr21:6708070 156.8 6708000 42778.4
    22:34:39.893 INFO ProgressMeter - chr21:6710070 157.1 6710000 42722.7
    22:34:54.968 INFO ProgressMeter - chr21:6712070 157.3 6712000 42667.2
    22:35:10.003 INFO ProgressMeter - chr21:6714070 157.6 6714000 42612.0
    22:35:25.052 INFO ProgressMeter - chr21:6716070 157.8 6716000 42557.0
    22:35:40.089 INFO ProgressMeter - chr21:6718070 158.1 6718000 42502.2
    22:35:55.210 INFO ProgressMeter - chr21:6720070 158.3 6720000 42447.1
    22:36:10.271 INFO ProgressMeter - chr21:6722070 158.6 6722000 42392.6
    22:36:25.280 INFO ProgressMeter - chr21:6724070 158.8 6724000 42338.4
    22:36:40.309 INFO ProgressMeter - chr21:6726070 159.1 6726000 42284.3
    22:36:55.345 INFO ProgressMeter - chr21:6728070 159.3 6728000 42230.3
    22:37:10.419 INFO ProgressMeter - chr21:6730070 159.6 6730000 42176.4
    22:37:25.532 INFO ProgressMeter - chr21:6732070 159.8 6732000 42122.4
    22:37:40.610 INFO ProgressMeter - chr21:6734070 160.1 6734000 42068.8
    22:37:55.668 INFO ProgressMeter - chr21:6736070 160.3 6736000 42015.4
    22:38:10.704 INFO ProgressMeter - chr21:6738070 160.6 6738000 41962.3
    22:38:25.798 INFO ProgressMeter - chr21:6790070 160.8 6790000 42220.0
    22:38:40.836 INFO ProgressMeter - chr21:6792070 161.1 6792000 42166.7
    22:38:55.849 INFO ProgressMeter - chr21:6794070 161.3 6794000 42113.7
    22:39:10.883 INFO ProgressMeter - chr21:6796070 161.6 6796000 42060.8
    22:39:25.933 INFO ProgressMeter - chr21:6798070 161.8 6798000 42007.9
    22:39:40.970 INFO ProgressMeter - chr21:6800070 162.1 6800000 41955.3
    22:39:56.015 INFO ProgressMeter - chr21:6802070 162.3 6802000 41902.8
    22:40:11.036 INFO ProgressMeter - chr21:6804070 162.6 6804000 41850.6
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    23:15:01.628 INFO ProgressMeter - chr21:7132071 197.4 7132000 36125.7
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    23:27:11.226 INFO ProgressMeter - chr21:7281088 209.6 7281000 34740.7
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    23:31:02.839 INFO ProgressMeter - chr21:7313089 213.4 7313000 34262.3
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    23:39:01.574 INFO ProgressMeter - chr21:7429090 221.4 7429000 33551.5
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    23:40:14.506 INFO ProgressMeter - chr21:7439090 222.6 7439000 33413.3
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    00:46:41.216 INFO ProgressMeter - chr21:8189115 289.1 8189000 28327.7
    00:46:55.641 INFO ProgressMeter - chr21:8191115 289.3 8191000 28311.0
    00:47:10.071 INFO ProgressMeter - chr21:8193115 289.6 8193000 28294.4
    00:47:24.535 INFO ProgressMeter - chr21:8195115 289.8 8195000 28277.8
    00:47:39.054 INFO ProgressMeter - chr21:8197115 290.0 8197000 28261.1
    00:47:53.524 INFO ProgressMeter - chr21:8199115 290.3 8199000 28244.5
    00:48:07.981 INFO ProgressMeter - chr21:8201115 290.5 8201000 28228.0
    00:48:22.449 INFO ProgressMeter - chr21:8203115 290.8 8203000 28211.4
    00:48:36.928 INFO ProgressMeter - chr21:8205115 291.0 8205000 28194.9
    00:48:51.362 INFO ProgressMeter - chr21:8207115 291.3 8207000 28178.5
    00:49:05.781 INFO ProgressMeter - chr21:8209115 291.5 8209000 28162.1
    00:49:20.200 INFO ProgressMeter - chr21:8211115 291.7 8211000 28145.8
    00:49:34.625 INFO ProgressMeter - chr21:8213115 292.0 8213000 28129.5
    00:49:49.065 INFO ProgressMeter - chr21:8215115 292.2 8215000 28113.1
    00:50:03.538 INFO ProgressMeter - chr21:8217115 292.5 8217000 28096.8
    00:50:18.000 INFO ProgressMeter - chr21:8219115 292.7 8219000 28080.5
    00:50:32.463 INFO ProgressMeter - chr21:8221116 292.9 8221000 28064.2
    00:50:46.906 INFO ProgressMeter - chr21:8223116 293.2 8223000 28048.0
    00:51:01.313 INFO ProgressMeter - chr21:8225116 293.4 8225000 28031.8
    00:51:15.747 INFO ProgressMeter - chr21:8227116 293.7 8227000 28015.7
    00:51:30.172 INFO ProgressMeter - chr21:8229116 293.9 8229000 27999.6
    00:51:44.606 INFO ProgressMeter - chr21:8231116 294.1 8231000 27983.5
    00:51:59.063 INFO ProgressMeter - chr21:8233117 294.4 8233000 27967.4
    00:52:13.567 INFO ProgressMeter - chr21:8235117 294.6 8235000 27951.2
    00:52:28.003 INFO ProgressMeter - chr21:8237117 294.9 8237000 27935.2
    00:52:42.453 INFO ProgressMeter - chr21:8239117 295.1 8239000 27919.2
    00:52:56.896 INFO ProgressMeter - chr21:8241117 295.3 8241000 27903.2
    00:53:11.361 INFO ProgressMeter - chr21:8243117 295.6 8243000 27887.2
    00:53:25.779 INFO ProgressMeter - chr21:8245117 295.8 8245000 27871.3

    This is really extremely slow and is not how it is supposed to be. I can provide you with VCF file which I used upon request.

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    Bhanu Gandham

    Hi danilovkiri

     

    I am looking into this and will get back to you shortly.

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    Bhanu Gandham

    Hi danilovkiri

    Can you please provide your vcf so we can debug? Here is info on how to share data with us: https://gatk.zendesk.com/hc/en-us/articles/360035889671

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    danilovkiri

    Hi Bhanu Gandham

    I have uploaded the archive. The name is danilovk_11Jun2020_main.tar

    There is another archive there named danilovk_11Jun2020.tar, but it is truncated due to connection loss.

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    Bhanu Gandham

    Hi danilovkiri

     

    We are looking into this and will get back to you shortly.

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    Bhanu Gandham

    Hi danilovkiri

    I have created a issue ticket for this. Since we have some other tickets in the queue that we are working on, we unfortunately cannot give you a timeline by when we will be able to fix this issue. However, rest assured this is on our radar and we will get to it. You can track its progress here: https://github.com/broadinstitute/gatk/issues/6663

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    Louis Bergelson

    danilovkiri Bhanu Gandham 

    Thank you for providing the test file.  We used it to identify and fix a critical performance bug. In the next GATK release your command will run 3 orders of magnitude faster.  (~5,000 sites per minute -> ~5,000,000).  It completed the test file in about 18 minutes.  This is still somewhat slower than you said it was in gatk3, but should hopefully be similar enough that it's not a problem.  

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    danilovkiri

    Bhanu Gandham Thank you!

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