Genome Analysis Toolkit

Variant Discovery in High-Throughput Sequencing Data

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Developed in the Data Sciences Platform at the Broad Institute, the toolkit offers a wide variety of tools with a primary focus on variant discovery and genotyping. Its powerful processing engine and high-performance computing features make it capable of taking on projects of any size. Learn more

23:52:16.676 WARN HDF5SVDReadCountPanelOfNormals - Exception encountered during creation of panel of normals (java.lang.IllegalArgumentException: Sample at index 0 does not have a positive sample median.

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    Gökalp Çelik

    Hi Zunqiu Wang

    Have you tried running by adjusting the minimum interval median percentile parameter to a higher value? Looks like filtering of intervals ended up zero valid results therefore it cannot perform a proper PON creation. You may want to QC your samples for PON creation before getting to this stage and see if there are bad samples that result in such error. If samples are hybrid capture in nature you can check CollectHsMetrics results to see possible outliers. 

    Regards. 

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