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Variant Discovery in High-Throughput Sequencing Data

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Developed in the Data Sciences Platform at the Broad Institute, the toolkit offers a wide variety of tools with a primary focus on variant discovery and genotyping. Its powerful processing engine and high-performance computing features make it capable of taking on projects of any size. Learn more

Running GATK mutect2 in slurm cluster has a memory error. My objective is to generate a VCF file from a given bam file

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    Gökalp Çelik

    Hi Jaume

    I see that you set your heapsize as 64G which is quite a bit more that what regular runs of Mutect2 really needs. Can you reduce that amount to 8 or 12G to see if that works?

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    Jaume

    Hi, thanks for answering. I have previously tried lower amounts as you suggest and it didn't work. Any other possible solutions?

    Thanks!

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    Gökalp Çelik

    Hi again. Actually the error message is coming directly from the cluster management

    slurmstepd: error: Detected 1 oom-kill event(s) in step 24271678.batch cgroup. Some of your processes may have been killed by the cgroup out-of-memory handler.

    It is possible that there is a limit to the amount of memory allowed for users on the cluster. Can you check with your IT?

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