I am using a sequencing technology different to Illumina and I will struggle to get >10 normals.
Please can you confirm that
i) using your PONs for mutect2 is still better (even it's a different technology) than not using any PON
ii) using your PONs (which contains several hundred normals from a different technology) would be better than using a PON from ~ 8 normals from the same technology??
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