Report file CreateExtendedIlluminaManifest Report file
Hi GATK/Picard team,
I am trying to convert illumina infinium gtc files to vcf using
java -jar picard.jar GtcToVcf \
INPUT=input.gtc \
REFERENCE_SEQUENCE=reference.fasta \
OUTPUT=output.vcf \
EXTENDED_ILLUMINA_MANIFEST=chip_name.extended.csv \
CLUSTER_FILE=chip_name.egt \
ILLUMINA_NORMALIZATION_MANIFEST=chip_name.bpm.csv \
SAMPLE_ALIAS=my_sample_alias \
In order to generetate the `EXTENDED_ILLUMINA_MANIFEST` file after much searching I found this command
java -jar $PICARD CreateExtendedIlluminaManifest \
INPUT=chip_name.bpm.csv \
OUTPUT=chip_name.extended.csv \
REPORT_FILE=??? \
CLUSTER_FILE=chip_name.egt \
REFERENCE_SEQUENCE=reference.fasta
By searching further, I came across two other community posts, only one of which is relevant and still seems unresolved: https://gatk.broadinstitute.org/hc/en-us/community/posts/4404560028571-How-do-I-run-CreateExtendedIlluminaManifest-myself-for-GtcToVcf-for-a-customized-Illumina-chip-
Its still not clear what file should be used with the `REPORT_FILE` flag. Can this be downloaded somewhere? Do we need to generate this file ourselves? Checking the source code has also been futile: https://github.com/broadinstitute/picard/blob/master/src/main/java/picard/arrays/illumina/CreateExtendedIlluminaManifest.java
Would it be possbile to add documentation on how to properly generate vcf files from illumina binary .gtc files and which steps are needed.
Best,
James Gallant
-
Thank you for your post, James Gallant ! I want to let you know we have received your question. We'll get back to you if we have any updates or follow up questions.
Please see our Support Policy for more details about how we prioritize responding to questions.
Please sign in to leave a comment.
1 comment