Acts as a large memory buffer between processes that are connected with unix pipes for the case that one or more processes produces or consumes their input or output in bursts. By inserting a large memory buffer between such processes each process can run at full speed and the bursts can be smoothed out by the memory buffer.
Example
java -jar SamToFastq.jar \ F=my.fastq \ INTERLEAVE=true | java -jar FifoBuffer | bwa mem -t 8 \dev\stdin output.bamCategory Other
Overview
Summary
Acts as a large memory buffer between processes that are connected with unix pipes for the case that one or more processes produces or consumes their input or output in bursts. By inserting a large memory buffer between such processes each process can run at full speed and the bursts can be smoothed out by the memory buffer.Example
java -jar SamToFastq.jar \ F=my.fastq \ INTERLEAVE=true | java -jar FifoBuffer | bwa mem -t 8 -Y reference.fasta \dev\stdin output.bam
FifoBuffer (Picard) specific arguments
This table summarizes the command-line arguments that are specific to this tool. For more details on each argument, see the list further down below the table or click on an argument name to jump directly to that entry in the list.
Argument name(s) | Default value | Summary | |
---|---|---|---|
Optional Tool Arguments | |||
--arguments_file |
read one or more arguments files and add them to the command line | ||
--BUFFER_SIZE |
536870912 | The size of the memory buffer in bytes. | |
--DEBUG_FREQUENCY |
0 | How frequently, in seconds, to report debugging statistics. Set to zero for never. | |
--help -h |
false | display the help message | |
--IO_SIZE |
65536 | The size, in bytes, to read/write atomically to the input and output streams. | |
--NAME |
Name to use for Fifo in debugging statements. | ||
--version |
false | display the version number for this tool | |
Optional Common Arguments | |||
--COMPRESSION_LEVEL |
5 | Compression level for all compressed files created (e.g. BAM and VCF). | |
--CREATE_INDEX |
false | Whether to create a BAM index when writing a coordinate-sorted BAM file. | |
--CREATE_MD5_FILE |
false | Whether to create an MD5 digest for any BAM or FASTQ files created. | |
--GA4GH_CLIENT_SECRETS |
client_secrets.json | Google Genomics API client_secrets.json file path. | |
--MAX_RECORDS_IN_RAM |
500000 | When writing files that need to be sorted, this will specify the number of records stored in RAM before spilling to disk. Increasing this number reduces the number of file handles needed to sort the file, and increases the amount of RAM needed. | |
--QUIET |
true | Whether to suppress job-summary info on System.err. | |
--REFERENCE_SEQUENCE -R |
Reference sequence file. | ||
--TMP_DIR |
One or more directories with space available to be used by this program for temporary storage of working files | ||
--USE_JDK_DEFLATER -use_jdk_deflater |
false | Use the JDK Deflater instead of the Intel Deflater for writing compressed output | |
--USE_JDK_INFLATER -use_jdk_inflater |
false | Use the JDK Inflater instead of the Intel Inflater for reading compressed input | |
--VALIDATION_STRINGENCY |
STRICT | Validation stringency for all SAM files read by this program. Setting stringency to SILENT can improve performance when processing a BAM file in which variable-length data (read, qualities, tags) do not otherwise need to be decoded. | |
--VERBOSITY |
INFO | Control verbosity of logging. | |
Advanced Arguments | |||
--showHidden |
false | display hidden arguments |
Argument details
Arguments in this list are specific to this tool. Keep in mind that other arguments are available that are shared with other tools (e.g. command-line GATK arguments); see Inherited arguments above.
--arguments_file
read one or more arguments files and add them to the command line
List[File] []
--BUFFER_SIZE
The size of the memory buffer in bytes.
int 536870912 [ [ -∞ ∞ ] ]
--COMPRESSION_LEVEL
Compression level for all compressed files created (e.g. BAM and VCF).
int 5 [ [ -∞ ∞ ] ]
--CREATE_INDEX
Whether to create a BAM index when writing a coordinate-sorted BAM file.
Boolean false
--CREATE_MD5_FILE
Whether to create an MD5 digest for any BAM or FASTQ files created.
boolean false
--DEBUG_FREQUENCY
How frequently, in seconds, to report debugging statistics. Set to zero for never.
int 0 [ [ -∞ ∞ ] ]
--GA4GH_CLIENT_SECRETS
Google Genomics API client_secrets.json file path.
String client_secrets.json
--help / -h
display the help message
boolean false
--IO_SIZE
The size, in bytes, to read/write atomically to the input and output streams.
int 65536 [ [ -∞ ∞ ] ]
--MAX_RECORDS_IN_RAM
When writing files that need to be sorted, this will specify the number of records stored in RAM before spilling to disk. Increasing this number reduces the number of file handles needed to sort the file, and increases the amount of RAM needed.
Integer 500000 [ [ -∞ ∞ ] ]
--NAME
Name to use for Fifo in debugging statements.
String null
--QUIET
Whether to suppress job-summary info on System.err.
Boolean true
--REFERENCE_SEQUENCE / -R
Reference sequence file.
File null
--showHidden / -showHidden
display hidden arguments
boolean false
--TMP_DIR
One or more directories with space available to be used by this program for temporary storage of working files
List[File] []
--USE_JDK_DEFLATER / -use_jdk_deflater
Use the JDK Deflater instead of the Intel Deflater for writing compressed output
Boolean false
--USE_JDK_INFLATER / -use_jdk_inflater
Use the JDK Inflater instead of the Intel Inflater for reading compressed input
Boolean false
--VALIDATION_STRINGENCY
Validation stringency for all SAM files read by this program. Setting stringency to SILENT can improve performance when processing a BAM file in which variable-length data (read, qualities, tags) do not otherwise need to be decoded.
The --VALIDATION_STRINGENCY argument is an enumerated type (ValidationStringency), which can have one of the following values:
- STRICT
- LENIENT
- SILENT
ValidationStringency STRICT
--VERBOSITY
Control verbosity of logging.
The --VERBOSITY argument is an enumerated type (LogLevel), which can have one of the following values:
- ERROR
- WARNING
- INFO
- DEBUG
LogLevel INFO
--version
display the version number for this tool
boolean false
GATK version 4.1.8.1-SNAPSHOT built at Sun, 2 Aug 2020 22:56:07 -0400.
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