Genome Analysis Toolkit

Variant Discovery in High-Throughput Sequencing Data

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Developed in the Data Sciences Platform at the Broad Institute, the toolkit offers a wide variety of tools with a primary focus on variant discovery and genotyping. Its powerful processing engine and high-performance computing features make it capable of taking on projects of any size. Learn more

CreateSequenceDictionary (Picard) Follow

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    Ryan Kyger


    Running picard CreateSequenceDictionary results in this error message:

    $ java -jar picard.jar CreateSequenceDictionary R=E.coli_K12_MG1655.fa O=E.coli_K12_MG1655.dict

    -bash: /home/ryan/miniconda3/envs/gatk4/bin/java: cannot execute binary file: Exec format error

    Is this due an issue with picard or the fasta file?

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