Metrics
Category Metrics
Overview
Metrics that are calculated during the process of marking duplicates within a stream of SAMRecords using the UmiAwareDuplicateSetIterator.This table summarizes the values that are specific to this metric.
Metric | Summary |
---|---|
LIBRARY |
Library that was used to generate UMI data. |
MEAN_UMI_LENGTH |
Number of bases in each UMI |
OBSERVED_UNIQUE_UMIS |
Number of different UMI sequences observed |
INFERRED_UNIQUE_UMIS |
Number of different inferred UMI sequences derived |
OBSERVED_BASE_ERRORS |
Number of errors inferred by comparing the observed and inferred UMIs |
DUPLICATE_SETS_IGNORING_UMI |
Number of duplicate sets found before taking UMIs into account |
DUPLICATE_SETS_WITH_UMI |
Number of duplicate sets found after taking UMIs into account |
OBSERVED_UMI_ENTROPY |
Entropy (in base 4) of the observed UMI sequences, indicating the effective number of bases in the UMIs. If this is significantly smaller than UMI_LENGTH, it indicates that the UMIs are not distributed uniformly. |
INFERRED_UMI_ENTROPY |
Entropy (in base 4) of the inferred UMI sequences, indicating the effective number of bases in the inferred UMIs. If this is significantly smaller than UMI_LENGTH, it indicates that the UMIs are not distributed uniformly. |
UMI_BASE_QUALITIES |
Estimation of Phred scaled quality scores for UMIs |
PCT_UMI_WITH_N |
The percentage of reads that contain an UMI that contains at least one N |
GATK version 4.6.0.0-33-gdffedfb built at Wed, 23 Oct 2024 21:44:48 -0400.
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